Aslanpençesi (Alchemilla ellenbergiana) Ekstrelerinin Antioksidan ve Antimikrobiyal Özelliklerinin Belirlenmesi

Yıl 2018, Cilt: 8 Sayı: 1, 113 - 121, 31.01.2018

Tuba Acet Kadriye Özcan

https://doi.org/10.17714/gumusfenbil.308430

Cited By: 6

Öz

Bu çalışmada, Gümüşhane bölgesi halk tıbbında
kullanılan Alchemilla ellenbergiana (aslanpençesi) bitkisinin farklı
polariteye sahip çözücülerle (hekzan, etil asetat, etanol ve metanol)
hazırlanmış ekstrelerinin toplam fenolik miktarları, antioksidan ve antimikrobiyal
aktiviteleri araştırıldı. Toplam fenolik miktarı gallik asit eşdeğeri olarak
spektrofotometrik yöntemle ölçüldü ve en yüksek değerler metanol ve etanol
ekstrelerinde tespit edildi. Ekstrelerin antioksidan aktiviteleri ABTS
[2,2'-azino-bis (3-etilbenzotiazolin-6-sülfonik asit)] ve DPPH
(2,2-difenil-1-pikrilhidrazil) yöntemleriyle spektrofotometrik olarak
belirlendi ve troloks eşdeğeri olarak hesaplandı. Antimikrobiyal aktivite disk
difüzyon yöntemiyle patojen mikroorganizmalar üzerine test edildi. Sonuç olarak
bitkinin etanol ve metanol ekstrelerinin antioksidan aktivitesi diğer
ekstrelerden anlamlı olarak yüksek bulundu (p<0.05). Benzer olarak, etanol
ve metanol ekstrelerinin antimikrobiyal aktivitesi de diğer eksterelerden
yüksek bulundu. 

Anahtar Kelimeler

Alchemilla ellenbergiana, antimikrobiyal aktivite, ABTS, antioksidan aktivite, disk difüzyon, DPPH

Determination of Antioxidant and Antimicrobial Properties of Lady's Mantle (Alchemilla ellenbergiana) Extracts

Öz

In this study, different polarity solvent (hexane, ethyl acetate,
ethanol and methanol) extracts of Alchemilla ellenbergiana (Lady's Mantle),
which used in folk medicine plant in Gümüşhane region, were investigated in
terms of total phenolic contents, antioxidant and antimicrobial activity.  Total phenolic contents were measured by
spectrophotometric metod as gallic acid equivalent and detect in the highest in
ethanol and methanol extracts. Antioxidant activity of extracts were determined
by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium
salt] and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods by spectrophotometric
metod and calculated as trolox equivalent. Antimicrobial activity was tested
against to pathogen microorganisms by disc diffusion method. As a result, it
has been found that ethanol and methanol extracts of plant have high
antioxidant and antimicrobial activity (p<0.05). Similarly, it has been
found that antimicrobial activity of ethanol and methanol extracts higher than
others.

Anahtar Kelimeler

ABTS, Alchemilla ellenbergiana, antimicrobial activity, antioxidant activity, disc diffusion, DPPH

Kaynakça

• Altundag, E. ve Ozturk, M., 2011, The 2nd International Geography Symposium GEOMED2010, Ethnomedicinal studies on the plant resources of east Anatolia, Procedia Social and Behavioral Sciences, 19, 756–777.
• Brand-Williams, W., Cuvelier, M., Berset, C., 1995, Use of free radical method to evaluate antioxidant activity, Food Science and Technology, 28, 25-30.
• CLSI (Clinical and Laboratory Standards), 2007, Performance standards for antimicrobial susceptibility testing. 17th Informational Supplement, M100-S17, 27:1.
• Davis, P.H., 1970. Lathyrus L. In: Davis PH (ed.) Flora of Turkey and the East Aegean Islands. Vol. 3, 328-369. Edinburgh: Edinburgh University Press.
• Denev, P., Kratchanova, M., Ciz, M., Lojek, A., Vasicek, O., Blazheva, D., Nedelcheva, P., Vojtek, L., ve Hyrsl, P., 2014, Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts. Acta Biochimica Plonica, 61, 359–367
• Gülçin, I., Topal, F., Sarıkaya, S. B. Ö., Bursal, E., Bilsel, G., ve Gören, A.C., 2011, Polyphenol Contents and Antioxidant Properties of Medlar (Mespilus germanica L..Academy of Chemistry of Globe Publications, EISSN:1307-6167.
• Güler, B., Manav, E. ve Ugurlu, E., 2015, Medicinal plants used by traditional healers in Bozuyuk (Bilecik–Turkey). Journal of Ethnopharmacology, 173, 39–47.
• Hayırlıoğlu-Ayaz, S., ve Beyazoğlu, O., 1997, The chromosome numbers of five new Alchemilla L. (Rosaceae) records for Turkey. Turkish Journal of Botany, 21, 385-389.
• http://www.tubives.com/index.php?sayfa=1&tax_id=3736
• Izmailow, R., 1981, Karyological studies in species of Alchemilla L. from the Calycinae Bus. (section Brevicaulon Rothm.), Acta Biologica Cracoviensia Series Botanica, 23, 117-130.
• Kaya, B. ve Artuvan, Y., 2016, Investigation of antioxidant and antimicrobial effect, phenolic compounds of Alchemilla cimilensis, El-Cezerî Journal of Science and Engineering, 3(1), 27-54.
• Nikolova, M., Dincheva, I., Vitkova A. ve Badjakov, I., 2012, Phenolic Acids and Free Radical Scavenging Activity Of Alchemilla Jumrukczalıca Pawl., International Journal of Pharmaceutical Sciences and Research, 3(3), 802-804.
• Ozbek, H., Acikara, O. B., Keskin, I., Kirmizi, N. I., Ozbilgin, S., Oz, B. E., Kurtul, E., Ozrenk, B. C., Tekin, M. ve Saltan, G., 2017, Evaluation of hepatoprotective and antidiabetic activity of Alchemilla mollis. Biomedicine & Pharmacotherapy, 86, 172-176.
• Ozdemir, E. ve Alpınar, K., 2015, An ethnobotanical survey of medicinal plants in western part of central Taurus Mountains: Aladaglar (Nigde-Turkey), Journal of Ethnopharmacology, 166, 53–65.
• Paksoy, M.Y., Selvi, S. ve Savran, A., 2015, Ethnopharmacological survey of medicinal plants in Ulukısla (Nigde-Turkey), Journal of Herbal Medicine, 6(1), 42-48.
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang,, M., ve Rice-Evans, C., 1999. Article in Free Radical Biology and Medicine, Doi: 10.1016/S0891-5849(98)00315-3. Source: Pubmed.
• Rothmaler, W., 1937, Systematische Vorarbeiten zu einer Monographie der Gattung Alchemilla (L.) Scop. VII. Aufteilung der Gattung und Nomenklatur, Feddes Repert, 42, 146–173.
• Saeed, A., Rehman, S.U., Akram, M., Bhatti, M.Z., Naz, R., Latif, A., Ali, A., Ahmad, A ve Saeed, A., 2016, Evaluation of Antioxidant Effects and Inhibitory Activity of Medicinal Plants against Lipid Peroxidation Induced by Iron and Sodium Nitroprusside in the Mouse Brain. Journal of the Chemical Society of Pakistan, 38, 333.
• Sargin, S. A., 2015, Ethnobotanical survey of medicinal plants in Bozyazı district of Mersin, Turkey. Journal of Ethnopharmacology, 173, 105–126.
• Slinkard, K. ve Singleton, V.L., 1977, Total Phenol Analysis: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture, 28, 49-55.
• Şeker Karatoprak, G., İlgün, S. ve Koşar, M., 2017, Phenolic composition, antioxidant and antimicrobial activities of Alchemilla mollis (Buser) Rothm, Chemical Biodiversty, 14, n/a, e1700150. Doi:10.1002/cbdv.201700150.
• Usta, C., Yildirim, A. B. ve Turker, A. U., 2014, Antibacterial and antitumour activities of some plants grown in Turkey. Pharmaceutical Biotechnology, Biotechnology & Biotechnological Equipment, 28(2), 306-315.
• Viegi, L., Pieroni, A., Guarrera, P.M. ve Vangelisti, R., 2003, A review of plants used in folk veterinary medicine in Italy as basis for a databank. Journal of Ethnopharmacology, 89(2-3), 221-244.
• Zengin, G., Guler, G. O., Aktumsek, A., Ceylan, R., Picot, C. M. N.ve Mahomoodally, M.F., 2015, Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey. Hindawi Publishing Corporation Advances in Pharmacological Sciences, Article ID 410675.