Demir (III) oksit (Fe2O3) nanopartiküllerinin genotoksisitesinin Drosophila hemositlerinde KOMET yöntemi ile araştırılması

Yıl 2021, Cilt: 11 Sayı: 3, 643 - 652, 15.07.2021

Burcin Yalcin , Merve Güneş , İbrahim Hakkı Ciğerci , Bülent Kaya

https://doi.org/10.17714/gumusfenbil.821244

Öz

Nanopartikül (NP) kaynaklı ürünlerin giderek çeşitlenmesi ve bu ürünlerin ekonomik, çevresel ve insan sağlığı yararına fayda sağlaması nedeni ile NP’lerin kullanımı yaygınlaşmıştır. Ancak bu yoğun kullanım beraberinde bazı endişelerin oluşmasına da yol açmıştır. NP’ler kimyasal bileşimlerine, yapılarına, partikül büyüklüklerine, yüzey alanlarına ve şekillerine göre farklı toksik etkiler gösterebilmektedir. Şekilleri ve boyutları NP’lerin hücresel alımları ve potansiyel toksisitesinde önemli belirleyicilerdir. Demir (III) oksit (Fe2O3) NP’leri, manyetik rezonans görüntülemede, ilaç dağıtımında, biyolojik sıvıların detoksifikasyonu gibi birçok biyomedikal ve biyomühendislik alanlarında kullanıma sahiptir. Bu çalışmada Fe2O3 NP’lerinin genotoksisitesinin partikül boyutu ve şekli ile ilişkisini belirlemek amacıyla Drosophila hemositleri ile KOMET (alkali tek hücre jel elektroforez) analizi gerçekleştirilmiştir. KOMET sonucuna göre, <50 nm boyutlu ve küre forma sahip Fe2O3 NP’leri ile <100 nm boyutlu ve çubuk forma sahip Fe2O3 NP’lerinin genotoksik etkileri karşılaştırıldığında istatistiksel olarak anlamlı bir farklılık bulunmamıştır. Ancak her iki Fe2O3 NP uygulamasında da çalışılan 3 farklı dozdan (1, 2 ve 5 mM) sadece 1 mM’lık uygulamada kontrol grubu distile suya göre istatistiksel olarak anlamlı düzeyde genoksisitenin indüklendiği belirlenmiştir.

Anahtar Kelimeler

Demir oksit, Drosophila, Genotoksisite, KOMET, Nanopartikül

Destekleyen Kurum

Akdeniz Üniversitesi / Afyon Kocatepe Üniversitesi

Proje Numarası

FBG-2019-4977 / 13.FENBİL.22

Teşekkür

Bu çalışma FBG-2019-4977 proje numarası ile Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir. Ayrıca çalışmada kullanılan Fe2O3 NP’leri Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenen 13.FENBİL.22 numaralı projeden temin edilmiştir.

Investigation of genotoxicity of iron (III) oxide (Fe2O3) nanoparticles in Drosophila hemocytes by COMET method

Öz

The use of nanoparticles (NPs) has become widespread due to the increasing diversification of products from NP and the benefit of these products for economic, environmental and human health. However, this overutilize has also led to some concerns. NPs can show different toxic effects depending on their chemical composition, structure, particle size, surface area and shape. Their shape and size are important determinants in the cellular uptake and potential toxicity of NPs. Iron (III) oxide (Fe2O3) NPs are used in many biomedical and bioengineering fields such as magnetic resonance imaging, drug delivery, detoxification of biological fluids. In this study, COMET (alkaline single cell gel electrophoresis) analysis was performed with Drosophila hemocytes to determine the relationship between the genotoxicity of Fe2O3 NPs and particle size and shape. According to the result of COMET, no statistically significant difference was found in terms of genotoxicity when Fe2O3 NPs with of <50 nm and sphere form and Fe2O3 NPs with of <100 nm and rod form were compared. However, in both Fe2O3 NP treatments, it was determined that only 1 mM dose (between 1, 2 and 5 mM) was induced statistically significant level of genoxicity compared to the control group distilled water.

Anahtar Kelimeler

Iron oxide, Drosophila, Genotoxicity, COMET, Nanoparticle

Proje Numarası

FBG-2019-4977 / 13.FENBİL.22

Kaynakça

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