Isolation and characterization of secondary metabolites from apolar fraction of Scabiosa sicula and evaluation of their antioxidant activities

Yıl 2021, Cilt: 11 Sayı: 3, 934 - 942, 15.07.2021

Hilal Kılınç

https://doi.org/10.17714/gumusfenbil.888739

Öz

In this study, the identification and characterization of Scabiosa sicula dichloromethane extract constituents were discussed for the first time. Moreover, this study is also providing an overview of the phytochemistry of Mediterranean medicinal plants. The phytochemical investigation of S. sicula dichloromethane extract was performed by HPLC and resulted in isolation of six compounds and five of them were identified as phenolic compounds and one as triterpene. The structures of the isolated compounds determined as luteolin-6-C-glucoside, luteolin-7-O-glucoside, caffeic acid, ursolic acid, 4-O-caffeoylquinic acid and 3,5-O-dicaffeoylquinic acid using spectroscopic analysis, including NMR and HR-MS techniques. Moreover, TEAC and DPPH assays were performed to evaluate the antioxidant activity of S. sicula’s dichloromethane extract. TEAC value was defined as the concentration of Trolox solution with the same amount of antioxidant potential found in the 1 mg/mL solution of the tested extract resulted to be 1.21 ± 0.01 mg/mL. DPPH activity was calculated as (4.42 μg/mL) while the corresponding methanol extract antiradical activity was 3.34 ± 0.01 μg/mL. The contribution to literature with this study is to increase the depth of knowledge about S. sicula on secondary metabolites isolated from apolar fraction and the results of this study will help reveal the medical potential of these species.

Anahtar Kelimeler

Caprifoliaceae, Scabiosa sicula, Secondary metabolites

Destekleyen Kurum

TUBİTAK

Proje Numarası

1059B191700874

Teşekkür

The author would like to thank TUBITAK 2219 Grant number: 1059B191700874

Scabiosa sicula’nın apolar fraksiyonundan ikincil metabolitlerin izolasyonu ve karakterizasyonu ve antioksidant aktivitelerinin değerlendirilmesi

Öz

Bu çalışmada, Scabiosa sicula türünün diklorometan ekstresi bileşenlerinin tanımlanması ve karakterizasyonu ilk kez tartışılmıştır. Ayrıca, bu çalışma Akdeniz şifalı bitkilerinin fitokimyası hakkında genel bir bakış sunmaktadır. S. sicula diklorometan ekstresinin fitokimyasal incelenmesi HPLC ile yapılmış ve izole edilen altı bileşikten beş tanesinin fenolik bir tanesininde triterpen yapısında olduğu NMR ve HR-MS teknikleri kullanılarak luteolin-6-C-glukozit, luteolin-7-O-glukozit, kafeik asit, ursolik asit, 4-O-kafeoilkinik asit ve 3,5-O- dikaffeoilkinik asit olarak belirlenmiştir. S. sicula’nın diklorometan ekstresinin antioksidant aktivitesi TEAC ve DPPH yöntemleri ile incelenmiştir. TEAC değeri, test edilen ekstraktın 1 mg/mL solüsyonunda bulunan aynı miktarda antioksidan potansiyeline sahip Trolox solüsyonunun konsantrasyonuna bağlı olarak 1.21 ± 0.01 mg/mL tanımlandı. DPPH aktivitesi (4.42 μg / mL) olarak hesaplanırken ona karşılık gelen metanol ekstresinin antiradikal aktivitesi 3.34 ± 0.01 μg/mL olarak ölçülmüştür. Scabiosa sicula diklorometan ekstresinin ikincil metabolitlerinin tanımlandığı bu çalışmanın literatüre sağladığı katkı, bu türün tıbbi potansiyelinin ortaya çıkarılmasına yardım edecektir.

Anahtar Kelimeler

Caprifoliaceae, Scabiosa sicula, İkincil metabolitler

Proje Numarası

1059B191700874

Kaynakça

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