The Effects of Pneumatic Tourniquet on Serum TAS, TOS, MDA and GSH Levels in Patients Undergoing Carpal Tunnel Syndrome Surgery

Yıl 2022, , 1024 - 1028, 27.09.2022

Halil Sezgin Semis , Sinan Çelik , Ahmet Kekeç

https://doi.org/10.37989/gumussagbil.1118587

Öz

This study was designed to reveal the changes in serum total oxidative stress (TOS), total antioxidant status (TAS), malondialdehyde (MDA) and glutathione (GSH) levels of pneumatic tourniquet application at different times of tourniquet in 25 patients who underwent carpal tunnel syndrome surgery.
Twenty-five patients who were diagnosed with carpal tunnel syndrome and underwent surgical intervention were included in the study, and pneumatic tourniquets were applied to all patients. The blood taken from the patients 10 minutes before the tourniquet application, 20 minutes after the tourniquet application and 10 minutes after the tourniquet was opened, was centrifuged at 3500 rpm and the serum was removed. TOS, TAS, MDA and GSH levels of serums obtained were measured in a spectrophotometer using the colorimetric method. The results obtained from the patients showed that MDA and TOS levels increased and GSH and TAS levels decreased after tourniquet applications.
As a result, how the ischemia due to tourniquet application, which is applied to increase surgical comfort, and reperfusion that will develop after the tourniquet is opened, on serum TOS, TAS, MDA and GSH levels in carpal tunnel surgery syndrome surgeries, which are frequently seen and commonly performed on patients with diagnosed today, change was determined by this study.

Anahtar Kelimeler

Glutathione, Carpal tunnel syndrome, Malondialdehyde, Total antioxidant status, Total oxidative stress

Karpal Tünel Sendromu Cerrahisi Uygulanan Hastalarda Pnömotik Turnikenin Serum TAS, TOS, MDA ve GSH Seviyeleri Üzerine Etkileri

Öz

Bu çalışma karpal tünel sendromu cerrahisi uygulanan 25 hastada pnömotik turnike uygulamasının serum total oksidatif stres (TOS), total antioksidan kapasite (TAS), malondialdehit (MDA) ve glutatyon (GSH) düzeylerinde turnikenin farklı zamanlarında meydana gelen değişimlerin ortaya konulmasını amaçlamak için tasarlanmıştır.
Çalışmaya karpal tünel sendromu tanısı alan ve cerrahi müdahale yapılan 25 hasta dahil edilmiş olup, hastalarının tamamında pnömotik turnike uygulandı. Hastalardan turnike uygulamasından 10 dakika önce, turnike uygulandıktan sonra 20. dakikada ve turnike açıldıktan 10 dakika sonra alınan kanlar 3500 rpm’de santrifüj edilerek serumları çıkarıldı. Elde edilen serumlardan TOS, TAS, MDA ve GSH düzeyleri kolorimetrik metodla spektrofotometrede bakıldı. Hastalardan elde edilen sonuçlar, turnike uygulamalarından sonra MDA ve TOS seviyelerinin yükseldiği, GSH ve TAS seviyelerinin azaldığını göstermiştir.
Sonuç olarak, günümüzde sıklıkla görülen ve teşhisi konulmuş hastalara yaygın olarak yapılan karpal tünel sendromu ameliyatlarında cerrahi konforu artırmak amacıyla başvurulan turnike uygulamasına bağlı gelişen iskeminin ve turnike açıldıktan sonra gelişecek olan reperfüzyonun serum TOS, TAS, MDA ve GSH düzeyleri üzerinde nasıl bir değişim ortaya çıkardığı bu çalışma ile belirlenmiş olup, yapılacak olan çalışmalara katkı sunacaktır.

Anahtar Kelimeler

Glutatyon, Karpal tünel sendromu, Malondialdehit, Total antioksidan kapasite, Total oksidatif stres

Kaynakça

• 1. Werner, R.A. and Andary, M. (2011). "Electrodiagnostic Evaluation of Carpal Tunnel Syndrome". Muscle and Nerve, 44 (4), 597-607.
• 2. Wipperman, J. and Goerl, K. (2016). "Carpal Tunnel Syndrome: Diagnosis and Management". American Family Physician, 94 (12), 993-999.
• 3. Padua, L, Coraci, D, Erra, C, Pazzaglia, C, Paolasso, I, Loreti, C, Caliandro, P. and Hobson-Webb, L.D. (2016). "Carpal Tunnel Syndrome: Clinical Features, Dagnosis, and Management". The Lancet Neurology, 15 (12), 1273-1284.
• 4. Genova, A, Dix, O, Saefan, A, Thakur, M. and Hassan, A. (2020). "Carpal Tunnel Syndrome: A Review of Literature". Cureus, 12 (3), e7333.
• 5. Marshall, S.C, Tardif, G. and Ashworth, N.L. (2007). "Local Corticosteroid Injection for Carpal Tunnel Syndrome". Cochrane Database of Systematic Reviews, (2). https://doi.org/10.1002/14651858.CD001554.pub2.
• 6. Seol, G.H, Kang, P, Lee, H.S. and Seol, G.H. (2016). "Antioxidant Activity of Linalool in Patients with Carpal Tunnel Syndrome ". BMC neurology, 16 (1), 1-6.
• 7. Kim, J.K, Koh, Y.D, Kim, J.S, Hann, H.J. and Kim, M.J. (2010). "Oxidative Stress in Subsynovial Connective Tissue of Idiopathic Carpal Tunnel Syndrome". Journal of Orthopaedic Research, 28 (11), 1463-1468.
• 8. Karakoc, M, Altindag, O, Keles, H. Soran, N. and Selek, S. (2007). "Serum Oxidative–Antioxidative Status in Patients with Ankylosing Spondilitis". Rheumatology International, 27 (12), 1131-1134.
• 9. Demirkol, A, Uludag, M, Soran, N, Aksoy, N, Gun, K, Incebıyık, S, Gurgen, I, Vural, M, Altun, Y. and Kesiktas, F.N. (2012). "Total Oxidative Stress and Antioxidant Status in Patients with Carpal Tunnel syndrome". Redox Report, 17 (6), 234-238.
• 10. Freeland, A.E, Tucci, M.A, Barbieri, R.A, Angel, M.F. and Nick, T.G. (2002). "Biochemical Evaluation of Serum and Flexor Tenosynovium in Carpal Tunnel Syndrome". Microsurgery, 22 (8), 378-385.
• 11. Sud, V. and Freeland, A.E. (2005). "Biochemistry of Carpal Tunnel Syndrome". Microsurgery 25 (1), 44-46.
• 12. Pisoschi, A.M. and Pop, A. (2015). "The Role of Antioxidants in the Chemistry of Oxidative Stress: A Review". European Journal of Medicinal Chemistry, 97, 55–74.
• 13. Karakoc, M, Altindag, O, Keles, H, Soran, N. and Selek, S. (2007). "Serum Oxidative–Antioxidative Status in Patients with Ankylosing Spondilitis". Rheumatology International, 27 (12), 1131-1134.
• 14. Day, B.J. (2008). "Antioxidants As Potential Therapeutics for Lung Fibrosis". Antioxidants and Redox Signaling, 10 (2), 355-370.
• 15. Najim, R.A, Sharquie, K.E. and Abu‐Raghıf, A.R. (2007). "Oxidative Stress in Patients with Behcet's Disease: I Correlation with Severity and Clinical Parameters". The Journal of Dermatology, 34 (5), 308-314.
• 16. Solmaz, D, Kozacı, D, İsmail, S, Taylan, A, Önen, F, Akkoç, N. and Akar, S. (2016). "Oxidative Stress and Related Factors in Patients with Ankylosing Spondylitis". European Journal of Rheumatology, 3 (1), 20.
• 17. Kim, J.K, Koh, Y.D, Kim, J.S, Hann, H.J. and Kim, M.J. (2010). "Oxidative Stress in Subsynovial Connective Tissue of Idiopathic Carpal Tunnel Syndrome". Journal of Orthopaedic Research, 28 (11), 1463-1468.
• 18. Ferrari, R.S. and Andrade, C.F. (2015). "Oxidative Stress and Lung Ischemia-Reperfusion Injury". Oxidative Medicine and Cellular Longevity, 590987, https://doi.org/10.1155/2015/590987.
• 19. Küçükler, S, Kandemir, F.M, Özdemir, S, Çomaklı, S. and Caglayan, C. (2021). "Protective Effects of Rutin Against Deltamethrin-induced Hepatotoxicity and Nephrotoxicity in Rats via Regulation of Oxidative Stress, Inflammation, and Apoptosis". Environmental Science and Pollution Research, 28 (44), 62975-62990.
• 20. Sahna, E, Parlakpinar, H, Ozturk, F, Cigremis, Y. and Acet, A. (2003). "The Protective Effects of Physiological and Pharmacological Concentrations of Melatonin on Renal Ischemia-eperfusion Injury in Rats". Urological Research, 31 (3), 188-193.
• 21. Kim, T.H. and Lee, S.M. (2010). "The Effects of Ginseng Total Saponin, Panaxadiol and Panaxatriol on Ischemia/Reperfusion Injury in Isolated Rat Heart". Food and Chemical Toxicology, 48 (6), 1516-1520.
• 22. Kinaci, M.K, Erkasap, N, Kucuk, A, Koken, T. and Tosun, M. (2012). "Effects of Quercetin on Apoptosis, NF-κB and NOS Gene Expression in Renal Ischemia/Reperfusion Injury". Experimental and Therapeutic Medicine, 3 (2), 249-254.
• 23. Senturk, G.E, Erkanli, K, Aydin, U, Yucel, D, Isiksacan, N, Ercan, F. and Arbak, S. (2013). "The Protective Effect of Oxytocin on Ischemia/Reperfusion Injury in Rat Urinary Bladder". Peptides, 40, 82-88.
• 24. Korkmaz, A. and Kolankaya, D. (2009). "The Protective effects of Ascorbic Acid Against Renal Ischemia-Reperfusion Injury in Male Rats". Renal Failure, 31 (1), 36-43.