Prostat Karsinomunda Enerji Metabolizmasının Yeniden Programlanması: İn Siliko Analiz

Yıl 2020, Cilt: 9 Sayı: 4, 350 - 356, 02.01.2021

Mustafa Emre Ercın Ender Şimşek

https://doi.org/10.37989/gumussagbil.797335

Cited By: 1

Öz

Prostat karsinomunun, dikkate değer bir onkojenik modüle edilmiş metabolik programlama sergileyen, hipoksik ve lipojenik bir solid tümör olduğu bilinmektedir. Glukoz alımının ve Warburg etkisi olarak bilinen aerobik glikolizin artması hipoksik tümörlerin ana metabolik değişiklikleridir. Protein, nükleik asit ve lipid biyosentezi, kanserin metabolik yeniden programlanması ile ilişkili diğer metabolik süreçlerdir. Prostat karsinomunda “Warburg” etkisine ek olarak, alternatif metabolik yolaklarda yağ asitleri, glutamin ve mitokondriyal oksidatif fosforilasyon tümörün progresyonunda önemli katılımcılar olarak kabul edilir. Bu çalışmanın amacı, az diferansiye (seminal vezikül invazyonlu) ve iyi diferansiye prostat karsinomlarında enerji metabolizmasının programlanmasını araştırmaktır. GSE32448 mikrodizi verileri “Gene Expression OmniBus" tan indirildi. Gen ekspresyon seviyesindeki farklılıklar, 40 prostat kanseri örneğinden elde edilen dokuların mRNA transkriptlerinin yeniden analiz edilmesiyle üretildi. R programı kullanılarak biyoenformatik analiz ile "Biobase", "Limma" ve "Geoquery" kütüphaneleri elde edildi. Yağ asidi metabolizması ile ilgili genlerde istatistiksel olarak anlamlı farklılıklar bulunmuştur. Prostat kanserinde lipid metabolizmasının rolüne ilişkin artan farkındalık, bu maligniteye karşı daha iyi tedavi stratejilerinin geliştirilmesine yol açabilir.

Anahtar Kelimeler

Biyoinformatik, Metabolik yeniden programlama, Moleküler Patoloji, Prostat Kanseri

Programming Of Energy Metabolism In Prostate Carcinoma: In Silico Analysis

Öz

Prostate carcinoma is known to be a hypoxic and lipogenic solid tumor, exhibiting a remarkable oncogenic modulated metabolic programming. Increasing intake of glucose and aerobic glycolysis, called the Warburg effect, are main metabolic changes in hypoxic tumors. Protein,nucleid acid , and lipid biosynthesis are the other metabolic processes associated with cancer metabolic rewiring. In addition to “Warburg effect” in prostate carcinoma, fatty acids, glutamine, and mitochondrial oxidative phosphorylation in alternative metabolic pathways are considered main contributors to tumorigenesis. The aim of this study is to investigate reprogramming of energy metabolism in well and poorly differentiated prostate carcinomas with seminal vesical invasion. The GSE32448 gene’s microarray data were downloaded from the "Gene Expression OmniBus". Differences in gene expression levels were generated by re-analyzing the mRNA transcripts of tissues obtained from 40 patients specimens. "Biobase", "Limma" and "Geoquery" libraries were obtained with bioinformatics analysis using R program. Statistically significant differences were found in genes related to fatty acid metabolism. Increased awareness of the role of lipid metabolism in prostate cancer can lead to developing better treatment strategies against this malignancy.

Anahtar Kelimeler

Bioinformatic, Metabolic reprogramming, Molecular pathology, Prostate cancer

Kaynakça

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