Antioksidant, Antimicrobial And Cytotoxic Activities Of Erica bocquetıı p.f. stevens and Erica arborea L.

Yıl 2013, Cilt: 2 Sayı: 1, 52 - 65, 01.01.2013

Bijen Kıvçak Tuğce Fafal Erdoğan , Tuba Gönenç Aysun Pabuçcuoğlu Esma Oran Ferdane Kahraman Tansel Öztürk

Öz

The aim of this study was to investigate in vitro antimicrobial, antioxidant, and cytotoxic activities of n-hexanoic, ethanolic, methanolic, ethyl acetate, and aqueous extracts of the aerial parts of Erica arborea L. and Erica bocquetii P.F. Stevens known to be endemic species for Turkey. The antimicrobial activity was investigated by disc diffusion method, and the minimal inhibitory concentration (MIC) by Broth Microdilution method. The antioxidant activity was determined by an improved assay based on the decolorization of the radical monocation of [2,2/-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS+). The cytotoxic activities were evaluated using Brine shrimp bioassay. The all extracts both species of Erica were also found to possess antimicrobial activity against some of the bacteria, but no activity was observed against the yeast. The antioxidant potentials of the extracts of E. bocquetii were obtained two times higher than those of E. arborea. Erica extracts did not exhibit the cytotoxic activity against Brine shrimp.

Anahtar Kelimeler

Erica bocquetii, Erica arborea, Antioxidant Activity, Antimicrobial Activity, Cytotoxicity, Brine Shrimp

Erıca bocquetıı p. F. Stevens And Erıca arborea L.’ nın Antioksidan, Antimikrobiyal Ve Sitotoksik Aktiviteler

Öz

Araştırmanın amacı Erica arborea L. ve Türkiye için endemik olarak bilinen Erica bocquetii P.F. Stevens' in toprak üstü kısımlarının n-hekzan, etanol, metanol, etil asetat ve su ekstrelerinin in vitro antimikrobiyal, antioksidan ve sitotoksik aktivitelerini araştırmaktır. Antimikrobiyal aktivite disk difüzyon metodu ve Broth Tüp Dilüsyon metodunun minimum inhibitör konsantrasyonu (MIC) ile araştırılmıştır. Antioksidan aktivite [2,2/-azinobis-(3-etilbenzotiazolin-6-sülfonik asit)] (ABTS+) radikal mono katyonunun dekolorizasyonu esas alınarak yapılan bir analiz ile belirlenmiştir. Sitotoksik aktiviteler Brine shrimp biyolojik denemeleri ile gerçekleştirilmiştir. Erica türlerinin tüm ekstrelerinin bazı bakterilere karşı antimikrobiyal aktiviteye sahip olduğu bulundu, fakat mayaya karşı aktivite gözlenmedi. E. bocquetii ekstrelerinin antioksidan potansiyelleri E. arborea' nınkilerden iki kat yüksek olduğu saptandı. Erica ekstreleri Brine shrimp' e karşı sitotoksik aktivite göstermemiştir.

Anahtar Kelimeler

Erica bocquetii, Erica arborea, Antioksidan Aktivite, Antimikrobiyal Aktivite, Sitotoksisite, Brine Shrimp.

Kaynakça

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