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Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State

Yıl 2023, Cilt: 12 Sayı: 2, 719 - 726, 18.06.2023
https://doi.org/10.37989/gumussagbil.1183391

Öz

We investigated the changes in the gut microbiota depending on the type and frequency of use of carbohydrate, which is the main element in our diet, and the relationship of these changes with mood. Thus, we aimed to intervene in mood disorders, which are seen in approximately 6.2% of the population in our country, by improving the type and frequency of use of carbohydrates, which make up 60% of the daily diet. In the study, 197 young adults between the ages of 18-45; Questions were asked to determine the number and times of meals during the day, the basic nutrients that make up their meals, the foods they chose in case of mood changes they noticed, the types of carbohydrates they consumed and the frequency of consumption, and the data were collected in the digital environment. To increase the possibility of accurate and reliable data collection; The ideal number of questions was determined as 30 and the Cronbach Alpha coefficient was calculated as (0.78). SPSS 26 statistical program was used to determine the data and p<0.05 was considered statistically significant. Prolonged meal breaks, foods perceived as rewards, and moreover, consumption of foods that can be defined as "vain foods" whose content is far from benefit have been detected. Keeping the breaks between meals short, using nutrition not for "prolonged hunger", but for "hunger" and staying away from foods that can be defined as " vain foods" that contain no benefit, will bring us closer to mental and physical well-being.

Kaynakça

  • 1. Foster, J.A. and Neufeld, K.A.M. (2013). “Gut-Brain Axis: How the Microbiome İnfluences Anxiety and Depression”. Trends in Neuroscience, 36 (5), 305-312.
  • 2. Bennet, R, Eriksson, M. and Nord, C.E. (2002). “The Fecal Microflora of 1-3-Month-Old İnfants During Treatment with Eight Oral Antibiotics”. Infection, 30 (3), 158-160.
  • 3. Yatsunenko, T, Rey, F.E, Manary, M.J, Trehan, I, Dominguez-Bello, M.G, Contreras, M. and et al. (2012). “Human Gut Microbiome Viewed Across Age and Geography”. Nature, 486 (7402), 222-229.
  • 4. Desbonnet, L, Garrett, L, Clarke, G, Kiely, B, Cryan, C.F, and Dinan, T.G. (2010). “ Effects of the Probiotic Bifidobacterium İnfantis in the Maternal Separation Model of Depression”. Neuroscience, 170 (4), 1179-1188.
  • 5. Bravo, J.A, Forsythe, P, Chew, M.V, Escaravage, E, Savignac, H.M, Dinan, T.G. and et al. (2011). “Ingestion of Lactobacillus Strain Regulates Emotional Behavior and Central GABA Receptor Expression in a Mouse via the Vagus Nerve”. Proceedings of the National Academy of Sciences of the United States of America, 108 (38), 16050-16055.
  • 6. Neufeld, K.A.M, Kang, N, Bienenstock, J, and Foster, J.A. (2011).” Effects of İntestinal Microbiota on Anxiety-Like Behavior”. Communicative and İntegrative Biology, 4 (4), 492-496.
  • 7. Clarke, G, Grenham, S, Scully, P, Fitzgerald, P, Moloney, R.D, Shanahan, F. and et al. (2012). “The Microbiome-Gut-Brain Axis During Early Life Regulates the Hippocampal Serotonergic System in a Sex-Dependent Manner”. Molecular Psychiatry, 18 (6), 666-673.
  • 8. Crumeyrolle-Arias, M, Jaglin, M, Bruneau, A, Vancassel, S, Cardona, A, Daugé, V. and et al. (2014). “ Absence of the Gut Microbiota Enhances Anxiety-Like Behavior and Neuroendocrine Response to Acute Stress in Rats”. Psychoneuroendocrinology, 42, 207-217.
  • 9. Cryan, J.F. and Dinan, T.G. (2012). “Mind-Altering Microorganisms: The İmpact of the Gut Microbiota on Brain and Behaviour”. Nature Reviews Neuroscience, 13 (10), 701–712.
  • 10. Kaczmarczyk, M.M, Miller, M.J. and Freund, G.G. (2012). “The Health Benefits of Dietary Fiber: Beyond the Usual Suspects of Type 2 Diabetes Mellitus, Cardiovascular Disease and Colon Cancer”. Metabolism: Clinical and Experimental, 61 (8), 1058-1066.
  • 11. Dash, S, Clarke, G, Berk, M. and Jacka, F.N. (2015). “The Gut Microbiome and Diet in Psychiatry: Focus on Depression”. Current Opinion in Psychiatry, 28 (1), 1-6.
  • 12. Scheppach, W, Luehrs, H, and Menzel T. (2001). “Beneficial Health Effects of Low-Digestible Carbohydrate Consumption”. The British Journal of Nutrition, 1, 23-30.
  • 13. Ciarambino, T, Castellino, P, Paolisso, G, Coppola, L, Ferrara N, Signoriello, G. and et al. (2012). “Long Term Effects of Low Protein Diet on Depressive Symptoms and Quality of Life in Elderly Type 2 Diabetic Patients”. Clinical Nephrology, 78 (2), 122-130.
  • 14. Ruusunen, A, Lehto, S.M, Mursu, J, Tolmunen, T, Tuomainen, T.P, Kauhanen, J. and et al. (2014). “Dietary Patterns are Associated with The Prevalence of Elevated Depressive Symptoms and The Risk of Getting a Hospital Discharge Diagnosis of Depression in Middle-Aged or Older Finnish Men”. Journal of Affective Disorders, 159, 1-6.
  • 15. Gangwisch, J.E, Hale, L, Garcia, L, Malaspina, L, Opler, M.G, Payne, M.E, and et al. (2015). “High Glycemic İndex Diet as a Risk Factor for Depression: Analyses from The Women's Health Initiative”. The American Journal of Clinical Nutrition, 102 (2), 454-463.
  • 16. Lang, U.E, Beglinger, C, Schweinfurth, N, Walter, M, and Borgwardt, S. (2015). “ Nutritional Aspects of Depression”. Cellular Physiology and Biochemistry, 37 (3), 1029-1043.
  • 17. Wang, Z.H, Wu, W, Kang, S.S, Liu, X, Wu, Z, Peng, J, and et al. (2018). “ BDNF İnhibits Neurodegenerative Disease-Associated Asparaginyl Endopeptidase Activity via Phosphorylation by AKT”. Journal of Clinical İnvestigation İnsight, 3 (16), 1-21.
  • 18. Montuschi, P, Barnes, P.J, and Roberts, L.J (2004). “Isoprostanes: Markers and Mediators of Oxidative Stress”. Federation of American Societies for Experimental Biology Journal, 18 (15), 1791-1800.
  • 19. Huang, C, Momma, H, Cui, Y, Chujo, M, Otomo, A, Sugiyama, S. and et al. (2017). “Independent and Combined Relationship of Habitual Unhealthy Eating Behaviors with Depressive Symptoms: A Prospective Study”. Journal of Epidemiology, 27 (1), 42-47.
  • 20. Meule, A, Roeser, K, Randler, C. and Kübler, A. (2012). “Skipping Breakfast: Morningness-Eveningness Preference is Differentially Related to State and Trait Food Cravings”. Eating and Weight Disorders, 17 (4), 304-312.
  • 21. Antypa, N, Vogelzangs. N, Meesters, Y, Schoevers, R. and Penninx, BW. (2016). “Chrono-type Associations with Depression and Anxiety Disorders in a Large Cohort Study”. Depression and Anxiety, 33 (1), 75-83.
  • 22. Au, J. and Reece, J. (2017). “The Relationship Between Chrono-type and Depressive Symptoms: A Meta-Analysis”. Journal of Affective Disorders, 218, 93-104.
  • 23. Antonini, M, Conte, M.L, Sorini, C. and Falcone, M. (2019). “How the Interplay Between the Commensal Microbiota, Gut Barrier Integrity, and Mucosal Immunity Regulates Brain Autoimmunity”. Frontiers in Immunology, 10 (1937), 1-10.
  • 24. Dahl, W.J. and Stewart, M.L. (2015). “Position of the Academy of Nutrition and Dietetics: Health Implications of Dietary Fiber”. Journal of the Academy of Nutrition and Dietetics, 115 (11), 1861-1870.
  • 25. Taylor, A.M, Thompson, S.V, Edwards, C.G, Musaad, S.M.A, Khan, N.A. and Holscher, H.D. (2019). “Associations Among Diet, the Gastrointestinal Microbiota, and Negative Emotional States in Adults”. Nutritional Neuroscience, 23 (12), 983-992.
  • 26. Strandwitz, P, Kim, K.H, Terekhova, D, Liu, J.K, Sharma, A, Levering, J. and et al. (2019). “GABA Modulating Bacteria of the Human Gut Microbiota”. Nature Microbiology, 4 (3), 396-403.
  • 27. Faits, T, Walker, M.E, Rodriguez-Morato, J, Meng, H, Gervis, J.E, Galluccio, J.M. and et al. (2020). “Exploring Changes in The Human Gut Microbiota and Microbial-Derived Metabolites in Response to Diets Enriched in Simple, Refined, or Unrefined Carbohydrate-Containing Foods: A Post Hoc Analysis of a Randomized Clinical Trial”. The American Journal of Clinical Nutrition. 112 (6), 1631-1641.
  • 28. Compan, V, Walsh, B.T, Kaye, W. and Geliebter, A. (2015). “How Does the Brain Implement Adaptive Decision Making to Eat?”. The Journal of Neuroscience, 35 (41), 13868-13878.
  • 29. De Poy, L.M, McClung, C.A. and Logan, R.W. (2017). “Neural Mechanisms of Circadian Regulation of Natural and Drug Reward”. Neural Plasticity, 2017, 1-14.
  • 30. Feillet, C.A, Bainier, C, Mateo, M, Blancas-Velázquez, A, Salaberry, N.L, Ripperger, J.A. and et al. (2017). “Rev-Erbalpha Modulates the Hypothalamic Orexinergic System to İnfluence Pleasurable Feeding Behaviour in Mice”. Addiction Biology, 22 (2), 411-422.
  • 31. Hawkins, M.A.W, Keirns, N.G. and Helms, Z. (2018). “Carbohydrates and Cognitive Function”. Current Opinion in Clinical Nutrition and Metabolic Care, 21 (4), 302-307.
  • 32. Corwin, R.L. (2011). “The Face of Uncertainty Eats”. Current Drug Abuse Reviews, 4 (3), 174-181.
  • 33. Corwin, R.L, Avena, N.M. and Boggiano, M.M. (2011). “Feeding and Reward: Perspectives from Three Rat Models of Binge Eating”. Physiology & Behavior, 104 (1), 87-97.
  • 34. Avena, NM. (2010). “The Study of Food Addiction Using Animal Models of Binge Eating”. Appetite, 55 (3), 734–737.

Genel Duygu Durumuna Beslenme Sıklığının ve Besin Seçiminin Mikroflora Aracılı Etkisinin İncelenmesi

Yıl 2023, Cilt: 12 Sayı: 2, 719 - 726, 18.06.2023
https://doi.org/10.37989/gumussagbil.1183391

Öz

Beslenmemizdeki ana öğe olan karbohidrat tür ve kullanım sıklığına bağlı, bağırsak mikrobiyotası değişimleri ve bu değişimlerin duygu durumu ile ilişkisini araştırdık. Böylece ülkemizde her yıl nüfusun yaklaşık %6,2’ sinde görülen duygu durum bozukluklarına, günlük beslenmenin %60’ını oluşturan karbohidratların tür ve kullanım sıklığını iyileştirerek müdahale etmeyi hedefledik. Çalışmada 18-45 yaş arası 197 genç erişkine; gün içi öğün sayısı ve vakitleri, öğünlerini oluşturan temel besinleri, fark ettikleri duygu durumu değişikliklerinde seçtikleri besinleri, tükettikleri karbohidrat türleri ve tüketim sıklıklarını tespit edebilecek sorular yöneltildi ve veriler dijital ortamda toplandı. Doğru ve güvenilir veri toplama olasılığını artırmak için; ideal soru sayısı 30 olarak belirlendi ve Cronbach Alpha katsayısı (0,78) olarak hesaplandı. Bu çalışmada verilerin tespitinde SPSS 26 istatistik programı kullanıldı ve p<0,05 istatistiksel olarak anlamlı kabul edildi. Uzamış öğün araları, ödül olarak algılanan besinler ve dahası içeriği faydadan uzak “boşuna besinler” olarak tanımlanabilecek besinlerin tüketimi bulgulanmıştır. Öğün aralarını kısa tutmak ve beslenmeyi “uzamış açlıklar” için değil “açlık” için kullanmak ve içeriği faydadan uzak “boşuna besinler” olarak tanımlanabilecek besinlerden uzak durmak bizi zihinsel ve bedensel iyilik haline daha çok yaklaştıracaktır.

Kaynakça

  • 1. Foster, J.A. and Neufeld, K.A.M. (2013). “Gut-Brain Axis: How the Microbiome İnfluences Anxiety and Depression”. Trends in Neuroscience, 36 (5), 305-312.
  • 2. Bennet, R, Eriksson, M. and Nord, C.E. (2002). “The Fecal Microflora of 1-3-Month-Old İnfants During Treatment with Eight Oral Antibiotics”. Infection, 30 (3), 158-160.
  • 3. Yatsunenko, T, Rey, F.E, Manary, M.J, Trehan, I, Dominguez-Bello, M.G, Contreras, M. and et al. (2012). “Human Gut Microbiome Viewed Across Age and Geography”. Nature, 486 (7402), 222-229.
  • 4. Desbonnet, L, Garrett, L, Clarke, G, Kiely, B, Cryan, C.F, and Dinan, T.G. (2010). “ Effects of the Probiotic Bifidobacterium İnfantis in the Maternal Separation Model of Depression”. Neuroscience, 170 (4), 1179-1188.
  • 5. Bravo, J.A, Forsythe, P, Chew, M.V, Escaravage, E, Savignac, H.M, Dinan, T.G. and et al. (2011). “Ingestion of Lactobacillus Strain Regulates Emotional Behavior and Central GABA Receptor Expression in a Mouse via the Vagus Nerve”. Proceedings of the National Academy of Sciences of the United States of America, 108 (38), 16050-16055.
  • 6. Neufeld, K.A.M, Kang, N, Bienenstock, J, and Foster, J.A. (2011).” Effects of İntestinal Microbiota on Anxiety-Like Behavior”. Communicative and İntegrative Biology, 4 (4), 492-496.
  • 7. Clarke, G, Grenham, S, Scully, P, Fitzgerald, P, Moloney, R.D, Shanahan, F. and et al. (2012). “The Microbiome-Gut-Brain Axis During Early Life Regulates the Hippocampal Serotonergic System in a Sex-Dependent Manner”. Molecular Psychiatry, 18 (6), 666-673.
  • 8. Crumeyrolle-Arias, M, Jaglin, M, Bruneau, A, Vancassel, S, Cardona, A, Daugé, V. and et al. (2014). “ Absence of the Gut Microbiota Enhances Anxiety-Like Behavior and Neuroendocrine Response to Acute Stress in Rats”. Psychoneuroendocrinology, 42, 207-217.
  • 9. Cryan, J.F. and Dinan, T.G. (2012). “Mind-Altering Microorganisms: The İmpact of the Gut Microbiota on Brain and Behaviour”. Nature Reviews Neuroscience, 13 (10), 701–712.
  • 10. Kaczmarczyk, M.M, Miller, M.J. and Freund, G.G. (2012). “The Health Benefits of Dietary Fiber: Beyond the Usual Suspects of Type 2 Diabetes Mellitus, Cardiovascular Disease and Colon Cancer”. Metabolism: Clinical and Experimental, 61 (8), 1058-1066.
  • 11. Dash, S, Clarke, G, Berk, M. and Jacka, F.N. (2015). “The Gut Microbiome and Diet in Psychiatry: Focus on Depression”. Current Opinion in Psychiatry, 28 (1), 1-6.
  • 12. Scheppach, W, Luehrs, H, and Menzel T. (2001). “Beneficial Health Effects of Low-Digestible Carbohydrate Consumption”. The British Journal of Nutrition, 1, 23-30.
  • 13. Ciarambino, T, Castellino, P, Paolisso, G, Coppola, L, Ferrara N, Signoriello, G. and et al. (2012). “Long Term Effects of Low Protein Diet on Depressive Symptoms and Quality of Life in Elderly Type 2 Diabetic Patients”. Clinical Nephrology, 78 (2), 122-130.
  • 14. Ruusunen, A, Lehto, S.M, Mursu, J, Tolmunen, T, Tuomainen, T.P, Kauhanen, J. and et al. (2014). “Dietary Patterns are Associated with The Prevalence of Elevated Depressive Symptoms and The Risk of Getting a Hospital Discharge Diagnosis of Depression in Middle-Aged or Older Finnish Men”. Journal of Affective Disorders, 159, 1-6.
  • 15. Gangwisch, J.E, Hale, L, Garcia, L, Malaspina, L, Opler, M.G, Payne, M.E, and et al. (2015). “High Glycemic İndex Diet as a Risk Factor for Depression: Analyses from The Women's Health Initiative”. The American Journal of Clinical Nutrition, 102 (2), 454-463.
  • 16. Lang, U.E, Beglinger, C, Schweinfurth, N, Walter, M, and Borgwardt, S. (2015). “ Nutritional Aspects of Depression”. Cellular Physiology and Biochemistry, 37 (3), 1029-1043.
  • 17. Wang, Z.H, Wu, W, Kang, S.S, Liu, X, Wu, Z, Peng, J, and et al. (2018). “ BDNF İnhibits Neurodegenerative Disease-Associated Asparaginyl Endopeptidase Activity via Phosphorylation by AKT”. Journal of Clinical İnvestigation İnsight, 3 (16), 1-21.
  • 18. Montuschi, P, Barnes, P.J, and Roberts, L.J (2004). “Isoprostanes: Markers and Mediators of Oxidative Stress”. Federation of American Societies for Experimental Biology Journal, 18 (15), 1791-1800.
  • 19. Huang, C, Momma, H, Cui, Y, Chujo, M, Otomo, A, Sugiyama, S. and et al. (2017). “Independent and Combined Relationship of Habitual Unhealthy Eating Behaviors with Depressive Symptoms: A Prospective Study”. Journal of Epidemiology, 27 (1), 42-47.
  • 20. Meule, A, Roeser, K, Randler, C. and Kübler, A. (2012). “Skipping Breakfast: Morningness-Eveningness Preference is Differentially Related to State and Trait Food Cravings”. Eating and Weight Disorders, 17 (4), 304-312.
  • 21. Antypa, N, Vogelzangs. N, Meesters, Y, Schoevers, R. and Penninx, BW. (2016). “Chrono-type Associations with Depression and Anxiety Disorders in a Large Cohort Study”. Depression and Anxiety, 33 (1), 75-83.
  • 22. Au, J. and Reece, J. (2017). “The Relationship Between Chrono-type and Depressive Symptoms: A Meta-Analysis”. Journal of Affective Disorders, 218, 93-104.
  • 23. Antonini, M, Conte, M.L, Sorini, C. and Falcone, M. (2019). “How the Interplay Between the Commensal Microbiota, Gut Barrier Integrity, and Mucosal Immunity Regulates Brain Autoimmunity”. Frontiers in Immunology, 10 (1937), 1-10.
  • 24. Dahl, W.J. and Stewart, M.L. (2015). “Position of the Academy of Nutrition and Dietetics: Health Implications of Dietary Fiber”. Journal of the Academy of Nutrition and Dietetics, 115 (11), 1861-1870.
  • 25. Taylor, A.M, Thompson, S.V, Edwards, C.G, Musaad, S.M.A, Khan, N.A. and Holscher, H.D. (2019). “Associations Among Diet, the Gastrointestinal Microbiota, and Negative Emotional States in Adults”. Nutritional Neuroscience, 23 (12), 983-992.
  • 26. Strandwitz, P, Kim, K.H, Terekhova, D, Liu, J.K, Sharma, A, Levering, J. and et al. (2019). “GABA Modulating Bacteria of the Human Gut Microbiota”. Nature Microbiology, 4 (3), 396-403.
  • 27. Faits, T, Walker, M.E, Rodriguez-Morato, J, Meng, H, Gervis, J.E, Galluccio, J.M. and et al. (2020). “Exploring Changes in The Human Gut Microbiota and Microbial-Derived Metabolites in Response to Diets Enriched in Simple, Refined, or Unrefined Carbohydrate-Containing Foods: A Post Hoc Analysis of a Randomized Clinical Trial”. The American Journal of Clinical Nutrition. 112 (6), 1631-1641.
  • 28. Compan, V, Walsh, B.T, Kaye, W. and Geliebter, A. (2015). “How Does the Brain Implement Adaptive Decision Making to Eat?”. The Journal of Neuroscience, 35 (41), 13868-13878.
  • 29. De Poy, L.M, McClung, C.A. and Logan, R.W. (2017). “Neural Mechanisms of Circadian Regulation of Natural and Drug Reward”. Neural Plasticity, 2017, 1-14.
  • 30. Feillet, C.A, Bainier, C, Mateo, M, Blancas-Velázquez, A, Salaberry, N.L, Ripperger, J.A. and et al. (2017). “Rev-Erbalpha Modulates the Hypothalamic Orexinergic System to İnfluence Pleasurable Feeding Behaviour in Mice”. Addiction Biology, 22 (2), 411-422.
  • 31. Hawkins, M.A.W, Keirns, N.G. and Helms, Z. (2018). “Carbohydrates and Cognitive Function”. Current Opinion in Clinical Nutrition and Metabolic Care, 21 (4), 302-307.
  • 32. Corwin, R.L. (2011). “The Face of Uncertainty Eats”. Current Drug Abuse Reviews, 4 (3), 174-181.
  • 33. Corwin, R.L, Avena, N.M. and Boggiano, M.M. (2011). “Feeding and Reward: Perspectives from Three Rat Models of Binge Eating”. Physiology & Behavior, 104 (1), 87-97.
  • 34. Avena, NM. (2010). “The Study of Food Addiction Using Animal Models of Binge Eating”. Appetite, 55 (3), 734–737.
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Duygu Vardağlı 0000-0002-9022-1338

Özlem Ortakcı 0000-0002-1304-7364

Yayımlanma Tarihi 18 Haziran 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 12 Sayı: 2

Kaynak Göster

APA Vardağlı, D., & Ortakcı, Ö. (2023). Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, 12(2), 719-726. https://doi.org/10.37989/gumussagbil.1183391
AMA Vardağlı D, Ortakcı Ö. Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi. Haziran 2023;12(2):719-726. doi:10.37989/gumussagbil.1183391
Chicago Vardağlı, Duygu, ve Özlem Ortakcı. “Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State”. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi 12, sy. 2 (Haziran 2023): 719-26. https://doi.org/10.37989/gumussagbil.1183391.
EndNote Vardağlı D, Ortakcı Ö (01 Haziran 2023) Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi 12 2 719–726.
IEEE D. Vardağlı ve Ö. Ortakcı, “Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State”, Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, c. 12, sy. 2, ss. 719–726, 2023, doi: 10.37989/gumussagbil.1183391.
ISNAD Vardağlı, Duygu - Ortakcı, Özlem. “Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State”. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi 12/2 (Haziran 2023), 719-726. https://doi.org/10.37989/gumussagbil.1183391.
JAMA Vardağlı D, Ortakcı Ö. Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi. 2023;12:719–726.
MLA Vardağlı, Duygu ve Özlem Ortakcı. “Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State”. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, c. 12, sy. 2, 2023, ss. 719-26, doi:10.37989/gumussagbil.1183391.
Vancouver Vardağlı D, Ortakcı Ö. Investigation of Microflora-Mediated Effect of Nutrition Frequency and Food Choice on General Affective State. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi. 2023;12(2):719-26.