Investigation of the Effects of Smoking on Blood Metabolic Enzyme Activities in Pregnant Individuals

Yıl 2024, Cilt: 13 Sayı: 1, 274 - 280, 27.03.2024

Esra Şentürk Gülpınar Aslan Ela Varol Murat Şentürk Metin Yıldız

https://doi.org/10.37989/gumussagbil.1183093

Öz

Pregnancy is process that causes many changes in the expectant mother, both biochemically and physiologically. One of the parameters in which biochemical change is most easily observed and interpreted is enzymatic changes.
In this study, blood samples were taken from two groups of pregnant and non-pregnant women in Ağrı. These groups were divided into two groups according to their smoking status and some metabolic enzyme levels were compared. In this context, serum carbonic anhydrase (CA), erythrocyte CA, serum catalase (CAT), erythrocyte CAT, serum acetylcholinesterase (AChE) and erythrocyte AChE enzyme levels were compared in pregnant and non-pregnant women.
Our study consists of 35 pregnant and 54 non-pregnant individuals living in Ağrı. Personal information forms were collected from the individuals participating in our study by filling in face-to-face interviews. 2 cc blood samples were taken from individuals who agreed to participate. Blood samples were centrifuged and serum and blood cells were separated from each other. Statistical analyzes were performed with the GraphPad Prism 9.
In this study, it was determined that the average age of the participants was 25 in pregnant individuals and 22 in non-pregnant individuals. It was determined that 83% of the pregnant women did not smoke and 96% didn’t have a chronic disease. It was determined that 6.31% of the non-pregnant individuals participating were smokers. According to the data obtained, both serum and cytoplasm results for all three enzymes of smoking women were lower. In the case of pregnancy, all values were decreased except cytoplasm AChE levels.

Anahtar Kelimeler

Pregnant women, Carbonic anhydrase, Catalase, Acetylcholinesterase, Glutathione peroxidase

Proje Numarası

SYO.20.003

Gebe Bireylerde Sigaranın Kan Metabolik Enzim Aktiviteleri Üzerine Etkilerinin İncelenmesi

Öz

Gebelik süreci hem biyokimyasal hem de fizyolojik olarak anne adayında birçok değişikliğe sebep olan bir süreçtir. Biyokimyasal değişimin en kolay gözlendiği ve yorumlandığı parametrelerden bir tanesi enzimatik değişimlerdir.
Bu çalışmada, Ağrı’da gebe ve gebe olmayan iki grup kadından kan örnekleri alındı. Bu gruplar sigara içme durumlarına göre ikişer gruba ayrılarak bazı metabolik enzim seviyeleri kıyaslanmıştır. Bu kapsamda kan serum karbonik anhidraz (CA), eritrosit CA, kan serum katalaz (CAT), eritrosit CAT, kan serum asetilkolinesteraz (AChE) ve eritrosit AChE enzim düzeylerinin gebelerde ve gebe olmayanlarda karşılaştırılması amacıyla yapılmıştır.
Çalışmamız, Ağrı il merkezinde yaşayan 35 gebe ve 54 gebe olmayan bireyden oluşmaktadır. Çalışmamıza katılan bireylerden kişisel bilgi formları yüz yüze görüşme ile doldurularak toplandı. Katılmayı kabul eden bireylerden 2 cc kan numunesi alındı. Kan örnekleri santrifüjlenerek serum ve kan hücreleri birbirinden ayrıldı. Elde edilen sonuçlar, GraphPad Prism 9 programı ile istatistiksel analizleri yapıldı.
Yaptığımız bu araştırmada katılımcıların yaş ortalamaları gebe bireylerde 25, gebe olmayanlarda ise 22 olduğu belirlendi. Gebelerin %83’ünün sigara içmediği ve %96’sının ise kronik hastalığının olmadığı tespit edildi. Çalışmaya katılan gebe olmayan bireylerin %6,31’inin ise sigara kullandığı belirlendi. Elde edilen verilere göre sigara içen bayanların üç enzim için de hem serum hem de sitoplazma sonuçları daha düşük çıkmıştır. Gebelik durumunda ise sitoplazma AChE seviyeleri hariç bütün değerler azalmıştır.

Anahtar Kelimeler

Asetilkolinesteraz, Gebeler, Karbonik Anhidraz, Katalaz

Destekleyen Kurum

AİÇÜ BAP

Proje Numarası

SYO.20.003

Teşekkür

Çalışmada sağladığı maddi detekten dolayı AİÇÜ-BAP birimine, numunelerin elde edilmesindeki yardım ve desteklerinden dolayı Dr. Umut GÖKSUGÜZEL'e teşekkür ediyoruz.

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