Tavşan Vajinal Yara Modelinde Propolis Uygulamasının Yara İyileştirici Etkisi

Öz

Amaç: Bu çalışmanın amacı, tavşan vajinal yara modelinde propolis uygulamasının yara iyileştirici etkisini değerlendirmektir. Gereç ve Yöntemler: Hayvanlar kontrol grubu ve propolis uygulama grubu olarak rastgele iki gruba ayrıldı ve her grupta 5'er adet tavşan kullanıldı. Tüm tavşanlarda vajinal yaralanma introitustan orta vajinal epitel ve stromaya kadar tam kat arka duvar insizyonu ile gerçekleştirildi. Çalışma grubundaki hayvanlara lokal olarak %15 saf Anadolu Propolisi uygulandı ve uygulamaya üç günde bir devam edildi. Kontrol grubuna herhangi bir uygulama yapılmadı. 21 gün sonunda tavşanlar sakrifiye edildi. Diseksiyon ile vajina ve çevresindeki perineal doku çıkarıldı. 3 mikron kalınlığında vajinal kesitler alındı. Bu kesitlerin bir kısmı hematoksilin-eozin (HE) ve Mason's Tricrome (MT) boyaması için normal lamlara, bir kısmı da anti-vasküler endotelyal büyüme faktörü (VEGF-1) immünohistokimya boyaması için pozitif yüklü bir lam üzerine alındı. Bulgular: Her iki grup fibrozis ve MT boyaması açısından karşılaştırıldı. Her iki grup arasında istatistiksel olarak anlamlı fark bulundu (p=0,039, p=0.039). Her iki grup inflamasyon ve VEGF açısından karşılaştırıldığında iki grup arasında istatistiksel olarak anlamlı fark bulunmadı (p=0,285, p=0,428). Sonuç: Birinci derece perineal yaraların iyileşme sürecinde yarada aktif kanama yoksa ve yara kenarları düzgün ve birbirine yakın ise propolis uygulaması olumlu katkı sağlayabilir.

Anahtar Kelimeler

• Hayvan Modeli
• propolis
• Yara iyileşmesi

Wound Healing Effect of Propolis Application in Rabbit Vaginal Wound Model

Öz

Objectives: This study aims to evaluate the wound healing effect of propolis application in a rabbit vaginal wound model. Material and Methods: Animals were randomly divided into two groups, a control group, and a propolis application group, and five rabbits were used in each group. In all rabbits, the vaginal injury was performed with a full-thickness posterior wall incision from the introitus to the mid-vaginal epithelium and stroma. Locally, 15% pure Anatolian Propolis was applied to the animals in the study group, and the application was continued every three days. No application was made to the control group. At the end of 21 days, the rabbits were sacrificed. By dissection, the vagina and surrounding perineal tissue were removed. Three micron-thick vaginal cross-sections were taken. Some of these sections were taken from standard slides for hematoxylin-eosin (HE) and Mason's Trichrome (MT) staining, and some of them were taken on a positively charged slide for anti-vascular endothelial growth factor (VEGF-1) immunohistochemistry staining. Results: Both groups were compared in terms of fibrosis and MT staining. A statistically significant difference was found between both groups (p=0.039, p=0.039). When both groups were compared in terms of inflammation and VEGF, no statistically significant difference was found between the two groups ( p=0.285, p=0.428 ). Conclusion: In the healing process of first-degree perineal wounds, if there is no active bleeding in the wound and the wound edges are smooth and close to each other, propolis application may contribute positively.

Anahtar Kelimeler

• Animal models
• wound healing
• propolis

Kaynakça

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