Tavşan Vajinal Yara Modelinde Propolis Uygulamasının Yara İyileştirici Etkisi

Yıl 2022, Cilt: 14 Sayı: 4, 231 - 241, 10.01.2023

Begüm Kurt Çaglar Yıldız Neşe Kurt Özkaya Tülay Koç Serkan Çelikgün

Öz

Amaç: Bu çalışmanın amacı, tavşan vajinal yara modelinde propolis uygulamasının yara iyileştirici etkisini değerlendirmektir.
Gereç ve Yöntemler: Hayvanlar kontrol grubu ve propolis uygulama grubu olarak rastgele iki gruba ayrıldı ve her grupta 5'er adet tavşan kullanıldı. Tüm tavşanlarda vajinal yaralanma introitustan orta vajinal epitel ve stromaya kadar tam kat arka duvar insizyonu ile gerçekleştirildi. Çalışma grubundaki hayvanlara lokal olarak %15 saf Anadolu Propolisi uygulandı ve uygulamaya üç günde bir devam edildi. Kontrol grubuna herhangi bir uygulama yapılmadı. 21 gün sonunda tavşanlar sakrifiye edildi. Diseksiyon ile vajina ve çevresindeki perineal doku çıkarıldı. 3 mikron kalınlığında vajinal kesitler alındı. Bu kesitlerin bir kısmı hematoksilin-eozin (HE) ve Mason's Tricrome (MT) boyaması için normal lamlara, bir kısmı da anti-vasküler endotelyal büyüme faktörü (VEGF-1) immünohistokimya boyaması için pozitif yüklü bir lam üzerine alındı.
Bulgular: Her iki grup fibrozis ve MT boyaması açısından karşılaştırıldı. Her iki grup arasında istatistiksel olarak anlamlı fark bulundu (p=0,039, p=0.039). Her iki grup inflamasyon ve VEGF açısından karşılaştırıldığında iki grup arasında istatistiksel olarak anlamlı fark bulunmadı (p=0,285, p=0,428).
Sonuç: Birinci derece perineal yaraların iyileşme sürecinde yarada aktif kanama yoksa ve yara kenarları düzgün ve birbirine yakın ise propolis uygulaması olumlu katkı sağlayabilir.

Anahtar Kelimeler

Hayvan Modeli, propolis, Yara iyileşmesi

Wound Healing Effect of Propolis Application in Rabbit Vaginal Wound Model

Öz

Objectives: This study aims to evaluate the wound healing effect of propolis application in a rabbit vaginal wound model.
Material and Methods: Animals were randomly divided into two groups, a control group, and a propolis application group, and five rabbits were used in each group. In all rabbits, the vaginal injury was performed with a full-thickness posterior wall incision from the introitus to the mid-vaginal epithelium and stroma. Locally, 15% pure Anatolian Propolis was applied to the animals in the study group, and the application was continued every three days. No application was made to the control group. At the end of 21 days, the rabbits were sacrificed. By dissection, the vagina and surrounding perineal tissue were removed. Three micron-thick vaginal cross-sections were taken. Some of these sections were taken from standard slides for hematoxylin-eosin (HE) and Mason's Trichrome (MT) staining, and some of them were taken on a positively charged slide for anti-vascular endothelial growth factor (VEGF-1) immunohistochemistry staining.
Results: Both groups were compared in terms of fibrosis and MT staining. A statistically significant difference was found between both groups (p=0.039, p=0.039). When both groups were compared in terms of inflammation and VEGF, no statistically significant difference was found between the two groups ( p=0.285, p=0.428 ).
Conclusion: In the healing process of first-degree perineal wounds, if there is no active bleeding in the wound and the wound edges are smooth and close to each other, propolis application may contribute positively.

Anahtar Kelimeler

Animal models, wound healing, propolis

Kaynakça

• 1. American College of Obstetricians and Gynecologists (ACOG). Practice Bulletin No. 165: Prevention and management of obstetric lacerations at vaginal delivery. Practice Guideline. Obstet Gynecol. 2016; 128: 1–15.
• 2. Oryan A, Alemzadeh E, Moshiri A. Potential role of propolis in wound healing: Biological properties and therapeutic activities. Biomed Pharmacother. 2018; 98:469-483.
• 3. Gómez-Caravaca, A. M., Gómez-Romero, M., Arráez-Román, D., Segura-Carretero, A., & Fernández-Gutiérrez, A. Advances in the analysis of phenolic compounds in products derived from bees. Journal of pharmaceutical and biomedical analysis.2006; 41(4): 1220–1234.
• 4. Toreti, VC, Sato, HH, Pastore, GM, Park, YK. (2013). Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evidence-based complementary Alternative Medicine. 2013;2013:697390.
• 5. Cao XP, Chen YF, Zhang JL, You MM, Wang K, Hu FL. Mechanisms underlying the wound healing potential of propolis based on its in vitro antioxidant activity. Phytomedicine. 2017; 34:76-84.
• 6. Wagh VD. Propolis: a wonder bees product and its pharmacological potentials. Adv Pharmacol Sci.2013; 2013: 308249.
• 7. Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Yaman SO, Akbulut K, Kilinc K, Deger O. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutr Cancer.2016;68:165-72. Doi:10.2026/j.phymed.2017.06.001.
• 8. Alperin, M., Feola, A., Meyn, L., Duerr, R., Abramowitch, S., & Moalli, P. (2010). Collagen scaffold: a treatment for simulated maternal birth injury in the rat model. American Journal of Obstetrics and Gynecology, 202(6), 589.e1-8.
• 9. Shveiky, D., Iglesia, C. B., Sarkar Das, S., Ben Menachem-Zidon, O., Chill, H. H., Ji, H., & Sandberg, K. (2020). Age-associated impairments in tissue strength and immune response in a rat vaginal injury model. International Urogynecology Journal, 31(7), 1435–1441.
• 10. Oz M, Cetinkaya N, Bas S, Korkmaz E, Ozgu E, Terzioglu GS, Buyukkagnıcı U, Akbay S, Caydere M, Gungor T. A randomized controlled experimental study of the efficacy of platelet-rich plasma and hyaluronic acid for the prevention of adhesion formation in a rat uterine horn model. Archives of Gynecology and Obstetrics. 2016; 294: 533–540.
• 11. Hooker GD, Taylor BM, Driman DK. Prevention of adhesion formation with use of sodium hyaluronate-based bioresorbable membrane in a rat model of ventral hernia repair with polypropylene mesh--a randomized, controlled study. Surgery 1999: 125: 211–216.
• 12. Kaya C,Sever N, Cengiz H, Yıldız S, Ekin M, Yasar L. A randomized controlled study of the efficacy of misoprostol and hyaluronic acid in preventing adhesion formation after gynecological surgery: a rat uterine horn model. European Journal of Obstetrics & Gynecology and Reproductive Biology.2014;176:44-9.
• 13. Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabetic Medicine. 2006;23:594-608.
• 14. Samuelsson E, Ladfors L, Lindblom BG, Hagberg H. A prospective observational study on tears during vaginal delivery: occurrences and risk factors. Acta obstetricia et gynecologica Scandinavica. 2002; 81: 44-49.
• 15. Hopkins LM, Caughey AB, Glidden DV, Laros RK. Racial/ethnic differences in perineal, vaginal and cervical lacerations. American journal of obstetrics and gynecology. 2005; 193:455-9.
• 16. Phillips C, Monga A. Childbirth and the pelvic floor: “the gynaecological consequences”,Reviews in Gynaecological Practice 2005;5:15-22.
• 17. McGuire JA, Crandall CL, Abramowitch SD, RAffaella De Vita. Inflation and rupture of vaginal tissue. Interface focus 2019;9:20190029.
• 18. Seijmonsbergen SAE, Sahami S, Lucas C, AD. Nonsuturing or skin adhesives versus suturing of the perineal skin after childbirth: A systematic review. BIRTH. 2015; 42: 100-15.
• 19. Eröksüz Y, Canpolat İ, Silici S. Comparison of healing effects of propolis to silver sulfadiazine on full thickness skin wounds in rabbits. F.Ü. Sağ. Bil. Derg. 2008; 22 : 17 – 20.
• 20. Pessolato AGT, Martins DS, Ambrosio CE, Mancanares CAF, Carvalho AF. Propolis and amnion reepithelialise second-degree burns in rats. Burns.2011; 37: 1192–1201.
• 21. Olczyk P, Komosinska-Vassev K, Szczotka K, Stojko J, Klimek K, Kozma EM. Propolis ınduces chondroitin/dermatan sulphate and hyaluronic acid accumulation in the skin of burned wound. Evidence-based complementary and alternative medicine.2013;2013:290675.
• 22. Fılho OM, Carvalho AC de. Application of propolis to dental sockets and skin wounds. The Journal of Nihon University School of Dentistry. 1990; 32:4–13.
• 23. Bretz WA, Chiego DJ, Marcucci MC, Cunha I, Custódio A, Schneider LG. Preliminary Report on the Effects of Propolis on Wound Healing in the Dental Pulp. Zeitschrift für Naturforschung C. 1998; 1;53(11– 12):1045–8.
• 24. Mozherenkov VP, Prokofeva GL. Apitherapy of eye disease. Vestn Oftalmol 1991; 107: 73-5.
• 25. Mozherenkov VP, Miniaeva TG. The use of products from bee rasing in ophthalmology and otorhinolaringology. Med Sestra. 1991;50: 47-51.
• 26. Han MC, Durmus AS, Karabulut E, Yaman I. Effects of turkish propolis and silver sulfadiazine on burn wound healing in rats. Revue de Médecine Vétérinaire. 2005; 156: 624-627.