Bazı Kiraz Anaçlarının Genetik Akrabalık İlişkilerinin RAPD Moleküler Markır Yöntemi İle Belirlenmesi

Yıl 2018, Cilt: 22 Sayı: 3, 326 - 334, 24.09.2018

Hasan Pınar Mehmet Yaman Hasan Cumhur Sarısu Aydın Uzun Merve Arefe Yiğit

https://doi.org/10.29050/harranziraat.410847

Cited By: 1

Öz

Bu
çalışmada kiraza anaç olarak kullanılan ve/veya kullanılacak olan bazı genotiplerin
RAPD moleküler markır yöntemi kullanılarak genetik akrabalıklarının belirlenmesi
amaçlanmıştır. Yirmi farklı kiraz anacının (9 adeti kuş kirazı) genetik benzerliklerinin
belirlemesinde 16 farklı RAPD primerinin kullanıldığı çalışmada, 16 primerden
92’si polimorfik olmak üzere 109 bant elde edilmiş ve ortalama polimorfizm
oranı %83.73 olarak belirlenmiştir. Elde edilen bulgulara göre kiraz anacı genotipleri arasındaki
genetik benzerlik 0.60-0.95 arasında değişmiş olup en
düşük benzerlik % 60 ile SL64 genotipi ile diğer tipler arasında, en yüksek
benzerlik oranı ise %95 ile Kuş Kirazı-3 ve Kuş Kirazı-4 genotiplerinden elde
edilmiştir. Elde edilen dendograma göre üç ana grup oluşmuş olup, birinci grup Kuş
Kirazı-2 (KK2), Kuş Kirazı-6 (KK6), Kuş Kirazı-7 (KK7) ve Kuş Kirazı-8 (KK8)
yer almıştır.  İkinci grupta Kuş Kirazı-3
(KK3), Kuş Kirazı-4 (KK4), Kuş Kirazı-5 (KK5), Kuş Kirazı-9 (KK9), Kuş
Kirazı-10 (KK10), İdris ve diğer yabancı anaçlar yer almıştır. Üçüncü grupta  sadece SL64 genotipi yer almıştır. Elde
edilen bulgular RAPD moleküler markır tekniğinin kiraz anaçları arasındaki
genetik ilişkilerin belirlenmesinde etkin bir şekilde kullanılabileceğini
göstermektedir.

Anahtar Kelimeler

Prunus avium L., Kiraz, Anaç, Genetik çeşitlilik, RAPD

Kaynakça

• Acunalp, S., 2012. Ekonomik öneme sahip yerli kiraz genotiplerinin SSR’a dayalı genetik karakterizasyonu. Yüksek Lisans Tezi, Ankara Üniversitesi, Ankara, 66s.
• Cai, Y.L., Cao, D.W., Zhao, G.F., 2007. Studies on genetic variation in cherry germplasm using RAPD analysis. Scientia Horticulturae, 111: 248–254.
• Cummins, J.N., Aldwinckle, S.H., 1983. Breeding apple rootstocks. In: J.Janick (ed.). Plant Breeding Rewiews, AVI Publ., Westport, Conn, p. 294-394.
• Demirsoy, L., Demir, T., Demirsoy, H., Okumuş, A., Kaçar, Y.A., 2008. Identification of some sweet cherry cultivars grown in Amasya by RAPD markers. Acta Horticulturae, 795: 147-153.
• Doyle, J.J., Doyle, J. L., 1990. Isolation of plant DNA from fresh tissue. Focus,12:13-15.
• Ercisli, S. 2004. A short review of the fruit germplasm resources of Turkey. Genetics Resources and Crop Evolution 51: 419–435.
• Ercisli, S., Esitken, A., Orhan, E., Özdemir, O., 2006. Rootstocks used for temparate fruit trees in Turkey: An overview. Sodininkyste ir Darzininkyste, 25 (3):27-33.
• Eriş, A., Barut, E., 2000. Ilıman İklim Meyveleri –1, Uludağ Üniversitesi Ders Kitabı. Bursa, No: 6, 83 s.
• Eroglu, D., Cakir., B. 2015. Molecular characterization of cherry genotypes and rootstocks by using Prunus SSR sequences. Austin Journal of Biotechnology & Bioengineering, 2(3): 1044.
• Fabbri, A., Hormaza, J.I., Polito, V.S., 1995. Random amplified polymorphic DNA analysis of olive cultivars. J Journal of the American Society for Horticultural Science, 120:538-542.
• Kafkas, S., Özgen, M., Doğan, Y., Özcan, B., Ercişli, S., Serçe, S., 2008. Molecular characterization of mulberry accessions in Turkey by AFLP markers. Journal of the American Society for Horticultural Science, 4: 593–597.
• Koc, A., Bilgener, Ş., 2013. Morphological characterization of cherry rootstock candidates selected from Samsun Province in Turkey. TurkishJournal of Agriculture and Forestry, 37(5), 575-584.
• Koc, A., Celik, Z., Akbulut, M., Bilgener, S., Ercisli, S., Gunes, M., Esitken, A., 2013. Morphological characterization of cherry rootstock candidates selected from Central and East Black Sea Regions in Turkey. The Scientific World Journal, doi: 10.1155/2013/916520
• Lagercrantz, U., Ellegren, H., Anderson, L., 1993. The abundance of various polymorphic microsatellite motifsdif- fers between plants and vertebrates. Nucleic Acids Research, 21:1111–1115.
• Landry, B.S., Li, R.Q., Cheung, W.Y., Granger, R.L., 1994. Pyloheny analysis of 25 apple rootstock using RAPD markes and tactical gene tagging. Theorotical and Applied Genetic, 89 :847-852.
• Lisek, A., Korbin, M., Rozpara, E., 2006. Using simple generated rapd markers to distinguish between sweet cherry cultivars. Journal of Fruit and Ornamentel Plant Research, 14 (1)58-59.
• Lisek, A., Rozpara, E., 2009. Identification and genetic diversity assessment of cherry cultivars and rootstocks using the ISSR-PCR technique. Journal of Fruit Ornamental Plant Research, 17: 95–106.
• Lu, Z,X., Reighard, G.L., Baird, W.V., Abbott, A.G., Rajapakse, S., 1996. Identification of peach rootstock cultivars by RAPD markers. HortScience, 31: 127-129.
• Melounová, M., Vejl, P., Sedlák, P., Reznerová, A., Tesařová, M., Blažek, J., Zoufalá, J., 2004. The variability of Venturia inaequalis CKE. Races in the Czech Republic and the accumulation of resistance genes in apple germplasm. Plant Soil and Environment, 50(9): 416–423.
• Mratanic, E., Fotiric-Aksıc, M., Jovkovic, R., 2012. Analysis of wild sweet cherry(Prunus avium L.) germplasm diversity in South-East Serbia. Genetika, 44(2): 259-268.
• Ognjanov,V., Ljubojevic, M., Ninic-Todorovic, J., Bosnjakovic, D., Barac, G., Cukanovic, J., Mladenovic, E., 2012. Morphometric diversty in dwarf sourcherry germplasm in Serbia. Journal of Horticultural Science & Biotechnology, 87(2): 117-122.
• Ortiz, A., Renaud, R., Calzada, I., Ritter, E., 1997. Analysis of plum cultivars with RAPD markes. The Journal of Horticultural Science and Biotechnology, 72: 1-9.
• Ozyurt, K. I., Akca, Y., Ercisli, S., 2013. Molecular characterization of Prunus mahaleb. L. rootstock canditates by ISSR markers. Genetika, 45(3): 717-726.
• Özbek, S.,1978. Özel Meyvecilik. Ç.Ü. Yay. Yay. AÜ Basımevi, Ankara, No:128, 486 s.
• Özçağıran, R., Ünal, A., Özeker, E. İsfendiyaroğlu, M., 2003. Ilıman İklim Meyve Türleri. Sert Çekirdekli Meyveler Cilt-I. Ege Üniversitesi Ziraat Fakültesi Yayınları No: 553, İzmir, 213s.
• Pavlovic, N., Zdravkovic, J., Cvikic, D., Zdravkovic, M., Adzic, S., Pavlovic, S., Surlan-Momirovic, G., 2012. Characterization of onion genotypes by use of RAPD markers. Genetika, 2: 269–278.
• Pınar, H., Ercisli, S., Ünlu, M., Bircan, M., Uzun, A., Keles, D., Baysal, F., Atlı, S, H., Yılmaz, U, K., 2015. Determination of genetic diversity among some almond accesion. Genetika, 1: 13-22.
• Rehder, A., 1940. A manual of cultivated trees and shrub shardy in North America exclusive of the subtropical and warmer temperate regions. 2nd ed. Macmillan, New York, New York, USA, 996p.
• Rom, R.C., Carlson, R.F., 1987. Rootstocks for fruit crops. New York: J Wiley. 494p.
• Schnell, R.J., Ronning, C,M., Knight Jr, R,J., 1995. Identification of cultivar sand validation of genetic relationships in Mangifera indica L. Using RAPD markers. Theorotical and Applied genetics, 90: 269-274.
• Sierotzki H, Eggenschwiler M, Boillat O, McDermott J.M. Gessler C., 1994. Detection of variation in virulence toward apple cultivars in natural populations of Venturia inaequalis. Phytopathology,84: 1005–1009.
• Smith, J.S.C., Smith, O.S., 1992. Finger printing crop varieties. Advances and Agrononomy, 47: 85-140.
• Stiles, J.L., Lemme, C., Sondur, S., Morshidi, M.B., Manshadt, R., 1993. Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars. Theoritical and Applied genetics, 85: 697-701.
• Tenzer, I., Gessler, C., 1997. Subdivision and genetic structure of four populations of Venturia inaequalis in Switzerland. European Journal of Plant Pathology , 103: 565–571.
• Tenzer, I., Gessler, C. 1999. Genetic diversity of Venturia inaequalis across Europe. European Journal of Plant Pathology, 105: 545–552.
• Türkoglu, Z., Koc, A., Ercisli, S., Bilginer, S., Akbulut, M., Yıldırım, N., Gercekcioglu, R., Esitken, A., Gunes., 2012. Genetic reletionship among Prunus rootsttocks for sweet cherry (Prunus avium L.) cultivars. Plant Genetic Resources-Characterization and Utilization, 10(2): 101-107.
• Warburton, M.L., Bliss, F.A., 1996. Genetic diversty in peach revealed by randomly ampliified polymorphic DNA (RAPD) markes and compared to inbreeding coefficient. Journal of the American Society for Horticultural Science, 121: 1012-1019.
• Webster, A.D., 2001. Rootstocks for temperate fruit crops: Current uses, future potential and alternative strategies. ActaHort, 557: 25–34.
• Welsh, J., McClelland, M., 1990. Finger printing genomes using PCR with arbitray primers. Nucleic Acids Research, 189: 7212-7218.
• Williams, J.G.K., Kubelik, A.R.K., Livak, J.L., Rafalski, J.A., Tingey, S.V., 1990. DNA polymorphisms amplified by random primers are useful as genetic markers. Nucleic Acids Research, 18: 6531-6535.
• Yaman, B. 2003. Yabani Kiraz (Cerasus avium (L.) Moench). Gazi Üniversitesi Orman Fakültesi Dergisi, 3(1): 114-122.