Evaluation of The Nematicidal Effects of Some Weed Leaf Extracts on The Root-Knot Nematode Meloidogyne incognita

Yıl 2024, Cilt: 28 Sayı: 3, 500 - 507, 28.09.2024

Fatma Gül Göze Özdemir

Öz

The aim of this study was to determine the toxicological potential of the methanol extract of creeping thistle (Cirsium arvense (L.) Scop.), milk thistle (Silybum marianum (L.) Gaertn.), greater plantain (Plantago major L.) and jimsonweed (Datura stramonium) plant against Meloidogyne incognita in vitro and under controlled conditions on tomato. The study was conducted with four concentrations (125, 250, 500 and 1000 ppm) for each weed plant. The study was set up in a randomized trial design with 5 replications. In vitro, 1 ml of suspension containing 20 second stage juvenile (J2) ml-1 was added to petri dishes containing nine ml of extract, and after 48 hours, dead individuals were counted and the mortality rate was recorded. Five days after the nematode-sensitive Gülizar F1 tomato seedlings were transplanted into the pots under controlled conditions, nematode inoculation was carried out with 500 J2 in each pot. Twenty four hours after nematode inoculation, methanol extracts of the plants was applied to the soil at 30 ml/pot concentration. Fifty days later, galls and egg masses in the roots were counted. In vitro, the highest J2 mortality was found in all plants at 1000 ppm concentration. At this concentration, the mortality effect of creeping thistle (81.6%), milk thistle (85.4%) and jimsonweed (84.2%) plants on J2 was found to be higher than broadleaf plantain (67.8%). Under controlled conditions, no significant difference was detected in the average number of galls and egg masses on tomato roots between 1000 and 500 ppm concentrations. The lowest number of galls and egg masses was determined in milk thistle and jimsonweed applications, and over 80% suppressive effect was found. Consequently, the extract of milk thistle and jimsonweed can be used effectively in the control of root knot nematode.

Anahtar Kelimeler

Methanol extract, Nematicidal effect, Milk thistle, Jimsonweed

Bazı yabancı otların yaprak ekstraklarının kök-ur nematodu Meloidogyne incognita üzerinde nematisidal etkilerinin değerlendirilmesi

Öz

Bu çalışmanın amacı, köygöçüren (Cirsium arvense (L.) Scop.), deve dikeni (Silybum marianum (L.) Gaertn.), sinir otu (Plantago major L.) ve şeytan elması (Datura stramonium)’nın metanol ekstraktının Meloidogyne incognita'ya karşı toksikolojik potansiyelini in vitro ve kontrollü koşullar altında domates üzerinde değerlendirmektir. Çalışma her bitki için dört konsantrasyon (125, 250, 500 ve 1000 ppm) ile yürütülmüştür. Çalışma tesadüf parselleri deneme deseninde 5 tekerrürlü olarak kurulmuştur. İn vitroda dokuz ml ekstrakt içeren petrilere 20 adet ikinci dönem larva (L2) ml-1 içeren 1 ml süspansiyon eklenmiştir ve 48 saat sonra ölü bireyler sayılarak ölüm oranı kaydedilmiştir. Kontrollü koşullar altında yürütülen denemede nematoda hassas Gülizar F1 domates fideleri saksılara şaşırtıldıktan beş gün sonra her saksıya 500 L2 ile nematod inokulasyonu gerçekleştirilmiştir. Nematod inokulasyonundan 24 saat sonra bitkilerin metanol ekstraktı 30 ml/saksı konsantrasyonda olacak şekilde toprağa uygulanmıştır. Elli gün sonra köklerdeki gal ve yumurta paketleri sayılmıştır. İn vitroda en yüksek L2 mortalitesi tüm bitkilerde 1000 ppm konsantrasyonunda tespit edilmiştir. Bu konsantrasyonda köygöçüren (%81.6), deve dikeni (%85.4) ve şeytan elması (%84.2) bitkilerinin L2 üzerindeki öldürücü etkisi sinir otundan (%67.8) yüksek bulunmuştur. Kontrollü koşullar altında domates köklerinde gal ve yumurta paketi sayısı ortalamasında 1000 ve 500 ppm konsantrasyonları arasında önemli bir fark saptanmamıştır. En düşük gal ve yumurta paketi sayısı ise deve dikeni ve şeytan elması bitkilerinin uygulamalarında belirlenmiş ve %80’in üzerinde baskılayıcı etki bulunmuştur. Çalışmada deve dikeni ve şeytan elması ekstraktının kök-ur nematodunun kontrolünde etkili bir şekilde kullanılabileceği sonucuna varılmıştır.

Anahtar Kelimeler

Metanol ekstrakt, Nematisidal etki, Devedikeni, Şeytan elması

Kaynakça

• Adekunle, O. K., Acharya, R., & Singh, B. (2007). Toxicity of pure compounds isolated from Tagetes minuta oil to Meloidogyne incognita. Australasian Plant Disease Notes, 2, 101-104.
• Adekunle, O. K., Singh, N., Kumar, N. & Singh B. (2006). Nematicidal action of some plant extracts against Meloidogyne incognita and isolation of nematicidal fraction from Plantago lanceolata. Pakistan Journal of Nematology, 25 (1): 189-197.
• Adekunle, O. K., & Akinlua, A. (2007). Nematicidal effects of Leucaena leucocephala and Gliricidia sepium extracts on Meloidogyne incognita infecting okra. Journal of Agricultural Sciences, 52(1), 53-63.
• Andrés, M. F., González-Coloma, A., Sanz, J., Burillo, J., & Sainz, P. (2012). Nematicidal activity of essential oils: a review. Phytochemistry Reviews, 11, 371-390.
• Ashraf, M. S., & Khan, T. A. (2010). Integrated approach for the management of Meloidogyne javanica on eggplant using oil cakes and biocontrol agents. Archives of Phytopathology and Plant Protection, 43(6), 609-614.
• Aydinli, G., & Mennan, S. (2014). Effect of some plant extracts on Meloidogyne arenaria Neal, 1889 (Tylenchida: Meloidogynidae) and tomato. Türkiye Entomoloji Dergisi, 38(3), 323-332.
• Chaudhary, K. K., Haile, A., Ayresea, Z. G., Semereab, G., & Weldegergish, T. (2013). Nematıcıdal actıvıty of erıtrean weed plants agaınst the Root-Knot Nematode Meloıdogyne incognıta.Nematropica, 43(2), 207-215.
• D’Addabbo, T., Carbonara, T., Argentieri, M. P., Radicci, V., Leonetti, P., Villanova, L., & Avato, P. (2013). Nematicidal potential of Artemisia annua and its main metabolites. European Journal of Plant Pathology, 137, 295-304.
• Dong, L., Huang, C., Huang, L., Li, X., & Zuo, Y. (2012). Screening plants resistant against Meloidogyne incognita and integrated management of plant resources for nematode control. Crop Protection, 33, 34-39.
• Elbadri, G. A., Lee, D. W., Park, J. C., Yu, H. B., & Choo, H. Y. (2008). Evaluation of various plant extracts for their nematicidal efficacies against juveniles of Meloidogyne incognita. Journal of Asia-Pacific Entomology, 11(2), 99-102.
• Erdoğuş, F. D. (2022). The effect of three plant extracts on Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae). Turkish Journal of Entomology, 46(2), 131-138.
• Finney, D. J. (1978). Statistical method in biological assay (No. Ed. 3).
• Charles Griffin & Company. Göze Özdemir, F. G., Tosun, B., Şanlı, A., & Karadoğan, T. (2022). Bazı Apiaceae uçucu yağlarının Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Meloidogynidae)'ya karşı nematoksik etkisi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 59(3), 529-539.
• Gürsili, P. A., & Yeşilkaya, B. (2020). An Antı-Inflammatory Herbal for Health and Dıseases: Sılybum marıanum (Mılk Thıstle). Internatıonal Refereed Academıc Journal of Sports, Health And Medıcal Scıences, 37, 28-41.
• Jones, J. T., Haegeman, A., Danchin, E. G., Gaur, H. S., Helder, J., Jones, M. G., ... & Perry, R. N. (2013). Top 10 plant‐parasitic nematodes in molecular plant pathology. Molecular plant pathology, 14(9), 946-961.
• Kabil, F. F., & Adam, M. (2020). Nematıcıdal actıvıty of garden cress bıo actıve ıngredıents agaınst Root knot nematode (Meloıdogyne incognita) Infected Tomato Transplants. Plant Archives, 20 (2), 9301-9310.
• Kaushik, P., & Goyal, P. (2008). In vitro evaluation of Datura innoxia (thorn-apple) for potential antibacterial activity. Indian Journal of Microbiology, 48, 353-357.
• Khan, Z., & Kim, Y. H. (2007). A review on the role of predatory soil nematodes in the biological control of plant parasitic nematodes. Applied soil ecology, 35(2), 370-379.
• Khan, M. R., & Haque, Z. (2011). Soil application of Pseudomonas fluorescens and Trichoderma harzianum reduces root-knot nematode, Meloidogyne incognita, on tobacco. Phytopathologia Mediterranea, 50(2), 257-266.
• Kiewnick, S., & Sikora, R. A. (2006). Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biological control, 38(2), 179-187.
• Kocaman, N., & Dabak, D. Ö. (2015). Hepatoprotektif bir ajan: Silymarin. Firat Tıp Dergisi, 20(3), 128-132.
• Kong, J. O., Lee, S. M., Moon, Y. S., Lee, S. G., & Ahn, Y. J. (2007). Nematicidal activity of cassia and cinnamon oil compounds and related compounds toward Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae). Journal of nematology, 39(1), 31.
• Mateeva, A., & Ivanova, M. (2000). Alternative methods for control of root-knot nematodes, Meloidogyne spp. In International Symposium on Chemical and Non-Chemical Soil and Substrate Disinfectation 532 (pp. 109-114).
• Moltmann, E. 1988. Kairomone im Wurzelexsudat Von Getreide: Ihre Bedeutung für die Wirtsfindung der Infektionslarven des 34 Getreidezystenaelchens Heterodera avenae (Wollenw.) und Ihre Charakterisierung. Hohenheim University 148 s.
• Nico, A. I., Jiménez-Dı́az, R. M., & Castillo, P. (2004). Control of root-knot nematodes by composted agro-industrial wastes in potting mixtures. Crop protection, 23(7), 581-587.
• Oka, Y., Ben-Daniel, B. H., & Cohen, Y. (2001). Nematicidal activity of powder and extracts of Inula viscosa. Nematology, 3(8), 735-742.
• Oka, Y., Ben-Daniel, B. H., & Cohen, Y. (2006). Control of Meloidogyne javanica by formulations of Inula viscosa leaf extracts. Journal of Nematology, 38(1), 46.
• Pérez, M. P., Navas‐Cortés, J. A., Pascual‐Villalobos, M. J., & Castillo, P. (2003). Nematicidal activity of essential oils and organic amendments from Asteraceae against root‐knot nematodes. Plant Pathology, 52(3), 395-401.
• Salim, H. A., Salman, I. S., Majeed, I. I., & Hussein, H. H. (2016). Evaluation of some plant extracts for their nematicidal properties against root-knot nematode, Meloidogyne sp. Journal of Genetic and Environmental Resources Conservation, 3, 241-244.
• Satti, A. A., & Nasr, O. E. (2006). Effect of neem (Azadirachta indica A. Juss) seed powder and aqueous extract on the control of some major foliage insect pests of eggplant. Al Buhuth, 10(1), 1-16.
• Taba, S., Sawada, J., & Moromizato, Z. I. (2008). Nematicidal activity of Okinawa Island plants on the root-knot nematode Meloidogyne incognita (Kofoid and White) Chitwood. Plant and Soil, 303, 207-216.
• Taştan, A., Fidan, E., & Tekbudak, İ. K. (2024). Şeytan Elması (Datura stramonium L.) ve Sirken (Chenopodium album L.) Etanol Ekstraktlarının Bitki Patojeni (Macrophomina phaseolina ve Rhizoctonia solani) Fungusları Üzerine Allelopatik Etkileri. Türk Tarım ve Doğa Bilimleri Dergisi, 11(2), 389-395.
• Tran, D. X., La, H. A., Do, T., Phung, T. T., Truong, N. M., Tran, D. K., & Khuat, H. T. (2016). Weed allelochemicals and possibility for pest management. International Letters of Natural Sciences, 56, 25-39.
• Tiring, G., Satar, S., & Özkaya, O. (2021). Sekonder metabolitler. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 35(1), 203-215.
• Tsay, T. T., Wu, S. T., & Lin, Y. Y. (2004). Evaluation of Asteraceae plants for control of Meloidogyne incognita. Journal of Nematology, 36(1), 36.
• Türkseven, S., Örnek, H., & Keser, M. (2021). Ispanakta zehirlenme vakalarına bağlı olarak Datura stramonium L.(şeytan elması)’un farklı gelişme evrelerinde atropin miktarlarının belirlenmesi. Türkiye Herboloji Dergisi, 24(2), 49-56.
• Vinodhini, S. M., Monisha, T., Arunachalam, P. P., Rajshree, S., Vignesh, P., Ebenezar, E. G., Seenivasan, N. (2019). Effect of plant extracts on root-knot nematode Meloidogyne incognita infecting tomato. International Journal of Current Microbiology and Applied Sciences, 8(6), 373-378.
• Whitehead, A.G. (1998). Plant nematode control. CAB International, Wallingford, UK. 384pp.
• Williamson, V. M., & Kumar, A. (2006). Nematode resistance in plants: the battle underground. TRENDS in Genetics, 22(7), 396-403.
• Yöntem, A., Yıldızdaş, D., Horoz, Ö. Ö., Ekinci, F., Mısırlıoğlu, M., Bilen, S., & Yılmaz, H. L. (2021). Çocuk Yoğun Bakım Ünitemizde İzlenen Zehirlenme Olgularının Değerlendirilmesi. Çocuk Acil ve Yoğun Bakım Dergisi, 8, 88-92.