IGF-1/CaC8I, LEP/NmuCI and PRLR/Hin1II Polymorphisms in Saanen Goats
Öz
Objective: This study was aimed to identify IGF-1, LEP and PRLR polymorphisms by PCR-RFLP method in Saanen goats.
Material and Methods: In the study, nasal samples were collected from 72 Saanen goats via swabs and DNA was extracted. Polymorphisms of IGF-1, LEP and PRLR were determined by PCR amplification followed by RFLP method using restriction enzymes Cac8I, NmuCI and Hin1II, respectively.
Results: LEP/NmuCI produced three genotypes as TT, TC and CC with frequencies 90.3, 6.9 and 2.8%, respectively and two alleles (T=93.8%, C=6.2%). The frequencies of genotype for PRLR gene were CC (90.3%), CT (5.5%) and TT (4.2%) and allele frequencies were C (93.1%) and T (6.9%). The IGF-1/Cac8I locus was monomorphic in Saanen goat population.
Conclusion: The present results may be useful for future studies conducted on goat genome with respect to IGF-1, LEP and PRLR genes.
Material and Methods: In the study, nasal samples were collected from 72 Saanen goats via swabs and DNA was extracted. Polymorphisms of IGF-1, LEP and PRLR were determined by PCR amplification followed by RFLP method using restriction enzymes Cac8I, NmuCI and Hin1II, respectively.
Results: LEP/NmuCI produced three genotypes as TT, TC and CC with frequencies 90.3, 6.9 and 2.8%, respectively and two alleles (T=93.8%, C=6.2%). The frequencies of genotype for PRLR gene were CC (90.3%), CT (5.5%) and TT (4.2%) and allele frequencies were C (93.1%) and T (6.9%). The IGF-1/Cac8I locus was monomorphic in Saanen goat population.
Conclusion: The present results may be useful for future studies conducted on goat genome with respect to IGF-1, LEP and PRLR genes.
Anahtar Kelimeler
DNA markers, PCR-RFLP, single nucleotide polymorphism, nasal swab
Destekleyen Kurum
Ege Üniversitesi ÖYP Birimi
Teşekkür
This paper is a part of the PhD thesis of Mustafa Can YILMAZ who was supported by Faculty Member Training Program (ÖYP).
Kaynakça
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