Molecular Assessment of Genetic Diversity and Bottleneck in Hair Goat Reared in Türkiye
Öz
Objective: Being the most preferred and geographically distributed in Türkiye, the Hair goat was screened at a molecular level to evaluate genetic diversity and population structure via microsatellite DNA markers. This paper also aimed to investigate the effects of genetic bottleneck to evaluate whether the Hair goat has maintained its effective population size in recent past.
Material and Methods: A total of 411 Hair goats were sampled from farms participating in the "Hair Goat Breeding" project, initiated by the General Directorate of Agricultural Research and Policies in Aydın and Denizli provinces. Sampled animals were genotyped with 18 microsatellite loci to assess genetic diversity, population structure, and genetic bottleneck.
Results: A total of 341 different alleles were observed across 18 microsatellite loci in which the highest number of alleles (26) and effective alleles (10.18) were detected in INRA005 and HSC loci, respectively. The average observed heterozygosity (0.73) was lower than the expected value (0.83), whereas all loci turned out to be highly informative (PIC>0.50). Factorial correspondence analysis separated animals into two groups, while a genetic admixture was detected between these groups. STRUCTURE analysis, on the other hand, confirmed that 411 animals were derived from three ancestral populations in which the third group is drawn due to admixed individuals. The Wilcoxon test and mode-shift indicator detected a lack of genetic bottleneck indicating that Hair goats reared in Türkiye have maintained their effective population size in recent past.
Conclusion: This study validates that used microsatellite markers are highly polymorphic and could be utilized for revealing genetic diversity in different local goat breeds. The findings recovered in this study could be integrated into breeding and conservation programs, while further studies should adopt SNP array technologies and next-generation sequencing platforms to reveal deeper knowledge about the genetic diversity and population structure of Anatolian goat breeds.
Anahtar Kelimeler
Microsatellite, genetic variation, small ruminant, SSR markers
Destekleyen Kurum
This study was produced from the project numbered ZRF-13009, supported by Aydın Adnan Menderes University BAP commission
Proje Numarası
ZRF-13009
Etik Beyan
This study was approved by the Aydın Adnan Menderes University Animal Experiments Local Ethics Committee (Approval no: 050-04/2012/102).
Teşekkür
We are grateful to Adnan Menderes University Scientific Research Projects Commission (Project Number: ZRF-13009) and the General Directorate of Agricultural Research and Policies for providing us with the necessary animal materials.
Kaynakça
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