Accuracy in Pedigree Records in Hair Goats: New Microsatellite Based Paternity Test Panels
Öz
Objective: The aim of the study was to establish microsatellite-based paternity test panels that can be used in paternity tests for Hair goat populations bred in Aydın and Denizli provinces, and to evaluate them in terms of paternity test parameters.
Material and Methods: The animal material of the study consisted of a total of 247 hair goats (42 bucks and 205 kids) in Hair goat farms in Aydın and Denizli provinces. The 18 microsatellites used in the study were evaluated in terms of molecular genetic parameters obtained from genotyping. After the evaluation, microsatellites were ranked from highest to lowest based on their individual exclusion probability values. Eighteen paternity test panels were created by sequentially adding a new microsatellite with a lower individual exclusion probability than the previous one to the microsatellite with the highest exclusion probability. Molecular genetic test statistics were obtained for the paternity test panels.
Results: In the study, 306 alleles were observed. The observed heterozygosity ratio (Ho) ranged from 0.39 to 0.95, while the expected heterozygosity ratio (He) ranged from 0.72 to 0.92. In the study, individual P-probability of exclusion (PE) values ranged from 0.316 to 0.719, while the combined probability of exclusion (CPE) values for the paternity test panels ranged from 0.7188 to 0.9999. Among the paternity test panels, Panel -7 and the following panels showed values above the threshold value reported in the literature in terms of the combined probability of exclusion.
Conclusion: According to the study findings, Panels 7 and 8, designed for paternity testing with fewer microsatellite markers, can be more cost-effective and practical for Hair goat populations compared to other panels. The findings obtained from the study make a significant contribution and provide a perspective for improving hand-mating practices. This is crucial within the framework of the "National Genetic Improvement Project for Small Ruminants at Breeders' Conditions" coordinated by the General Directorate of Agricultural Research and Policies.
Anahtar Kelimeler
Microsatellite, probability of exclusion, small ruminants, DNA
Destekleyen Kurum
Adnan Menderes University Scientific Research Projects Commission
Proje Numarası
ZRF-13002
Etik Beyan
This study was approved by the Aydın Adnan Menderes University Animal Experiments Local Ethics Committee (Approval no: 050-04/2012/103).
Teşekkür
We would like to thank Adnan Menderes University Scientific Research Projects Commission for providing financial support for this study. We are grateful to the breeders who participated in the "Hair Goat Breeding" sub-projects as part of the National Genetic Improvement Project for Small Ruminants under Breeders' Conditions, coordinated by the General Directorate of Agricultural Research and Policies, for providing animal material. Additionally, we appreciate the managers of Adnan Menderes University Agricultural Biotechnology and Food Safety Application and Research Centre for providing the necessary infrastructure facilities for laboratory studies.
Kaynakça
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