Associations of VLDLR Gene Polymorphism (intron 11, 392C > T) with Egg Production and Weight in Japanese Quails (Coturnix coturnix japonica)
Öz
Objective: This study aimed to assess the association between the VLDRLR gene intron 11 (392 C>T) polymorphism and egg production and weight in quails.
Material and Methods: Egg yield and weight were recorded over 90 days for 191 Japanese quails, which were genotyped using the restriction fragment length polymorphism technique for the VLDLR gene variation.
Results: The VLDLR gene was polymorphic due to conserving all possible genotypes (TT, TC, and CC). The TT genotype was the most common with a frequency of 0.70, while the frequency of the TC and CC genotype were 0.16 and 0.14, respectively. The mean 90-day egg production was 78.31, 76.07, and 73.94 in TT, TC, and CC genotypes, respectively, while the mean egg weight ranged from 915.80 (CC genotype) to 939.19 (TT genotype). Association analysis revealed a significant relationship between the VLDLR genotypes and egg production traits (P<0.05), while no significant relationship was detected for egg weight.
Conclusion: This study showed that the TT genotype for VLDLR gene intron 11 (392 C>T) polymorphism can be used in marker-assisted selection studies in order to increase egg production in quails.
Keywords: Candidate genes, egg traits, genetic variation, MAS, PCR-RFLP
Anahtar Kelimeler
candidate genes, egg traits, genetic variation, MAS, PCR-RFLP
Etik Beyan
No ethical permission was required since DNA was extracted from shed feather samples of animals kept in individual cages.
Kaynakça
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