Effects of Oregano and Cumin Essential Oils on Fermentation Quality, Aerobic Stability and In Vitro Metabolic Energy Contents of Alfalfa Silages

Yıl 2019, Cilt: 60 Sayı: 2, 117 - 123, 30.12.2019

Sibel Soycan Önenç Firdevs Korkmaz Turgud Aslı Turan Uçman

https://doi.org/10.29185/hayuretim.555562

Cited By: 1

Öz

Objective:The aim of this study was to determine the effects of the addition of
oregano and cumin essential oil to alfalfa on the fermentation quality, aerobic
stability and in vitro metabolic energy content.

Material and Methods:The alfalfa was harvested at the beginning of flowering (5th
cutting), and was wilted for 8-10 hours. The study was carried out as 4 replicates in 3
groups formed by without essential oil control, the addition of oregano and cumin
essential oils at 650 mg / kg.
The study was carried out under farmland conditions in a closed place (8±2 °C);
chemical and microbiological analysis were conducted on the silages uncovered on
the 120th day. Furthermore, aerobic stability test was carried out for 5 days.

Results: The addition of oregano and cumin essential oil decreased significantly
(P˂0.01) in the pH, water soluble carbohydrate and ammonia nitrogen amounts.
However, dry matter and lactic acid contents was increased (P˂0.01). In the study, the
addition of oregano and cumin essential oils was decreased in total mesophilic
aerobic bacteria, enterobacter, yeast and mould counts, while lactic acid bacteria
numbers was increased (P˂0.01).

Conclusion:The addition of oregano and cumin essential oils at 650 mg/kg,
promoted silage fermentation. In the aerobic period, there was decreased in the pH,
production of CO2, total mesophilic aerobic bacteria, yeast and mould counts, and
thus improved aerobic stability. Oregano essential oil is most effective in preventing
mould gro

Anahtar Kelimeler

Alfalfa, silage, oregano, cumin

Kekik ve Kimyon Uçucu Yağlarının Yonca Silajlarının Fermantasyon Kalitesi, Aerobik Stabilitesi ile Yem Değeri Üzerine Etkileri

Öz

Amaç: Bu araştırmanın amacı, yoncaya kekik ve kimyon uçucu yağı ilavesinin
fermantasyon kalitesi, aerobik stabilitesi ile in vitro metabolik enerji içerikleri üzerine
etkilerini belirlemektir.

Materyal ve Metot: Yonca, çiçeklenme başlangıcında (5. biçim) hasad edilip, 8-10
saat soldurulmuştur. Araştırma, katkı maddesi ilave edilmeyen kontrol, 650 mg/kg
düzeyinde kekik ve kimyon uçucu yağları ilave edilerek oluşturulan 3 grupta, 4
tekerrrür olarak yürütülmüştür. Çalışma, çiftlik koşullarında bir depoda (8±2 °C)
gerçekleştirilmiş, silolamanın 120. gününde açılan silajlara kimyasal ve mikrobiyolojik
analizler yapılmıştır. Ayrıca, 5 gün süre ile aerobik stabilite testi uygulanmıştır.

Bulgular: Kekik ve kimyon uçucu yağı ilavesi pH, suda çözülebilir karbonhidrat ve
amonyak azotu miktarlarını önemli düzeyde (P˂0.01) düşürmüştür. Oysa kuru madde
ve laktik asit içerikleri artmıştır (P˂0.01). Enzimde çözünen organik madde miktarları
ise başlangıç materyaline göre düşmüş, kontrole göre önemli düzeyde yükselmiştir
(P˂0.01). Araştırmada, toplam mezofilik aerobik bakteri, enterobakter, maya ve küf
sayıları, kekik ve kimyon uçucu yağı ilavesiyle düşmüş (P˂0.01), laktik asit bakteri
sayıları ise artmıştır (P˂0.01).

Sonuç: Yoncaya kekik ve kimyon uçucu yağlarının 650 mg/kg düzeyinde katılması,
silaj fermantasyonunu teşvik etmiş, aerobik stabiliteyi geliştirmiştir. Ancak, kekik
uçucu yağı küf gelişimini kimyon ise maya gelişimini önlemede daha ön plana
çıkmıştır.

Anahtar Kelimeler

Yonca, silaj, kekik

Kaynakça

• Akıncı Y. 2018. Kimyon uçucu yağının fiğ-yulaf silajının fermantasyon kalitesi, aerobik stabilitesi ve in vitro sindirilebilirlik üzerine etkisi. NKÜ Fen Bilimleri Enstitüsü YL tezi.
• Anonim, 1986. The analysis of agricultural material. Reference book:427- 428. London.
• Anonim, 2014. Mikrobiyoloji - Gıda ve hayvan yemleri - Mikroorganizmaların sayımı için yatay yöntem. Yayma plak tekniğiyle 30°C’ta koloni sayımı, TS EN ISO 4833.
• Basmacıoğlu H, Ergül M. 2002. Silaj mikrobiyolojisi. Hayvansal Üretim 43(1):12-24.
• Barker SB, Summerson WH. 1941. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138:535-554.
• Bulgurlu Ş, Ergül M. 1978. Yemlerin fiziksel kimyasal ve biyolojik analiz metotları. E.Ü. Baskı no: 127, İzmir.
• Busquet M, Calsamiglia S, Ferret A, Kamel C.2006. Plant Extracts Affect In Vitro Rumen Microbial Fermentation. Journal of Dairy Science. 89:761-771.
• Calsagmiglia S, Busquet M, Cardozo PW, Castillejos L, Ferret A. 2007. Invited review: Essential oils as modifiers of rumen microbial fermentation. Journal of Dairy Science. 90: 2580-2595.
• Cardoza PW, S Calsamiglia, A Ferret, C Camel. 2004. Effect of natural plant extracts on ruminal protein degration and profiles in fermentation continuous culture. Journal of Animal Science. 82:3230-3236.
• Cavallarin L, S Antoniazzi S, Borreani G, Tabacco E. 2005. Effects of wilting and mechanical conditioning on proteolysis in sainfoin (Onobrychis viciifolia Scop) wilted herbage and silage. Journal of Science Food Agriculture. 85:831–838.
• Chaves AV, Baah J, Wang Y, McAllister TA, Benchaar C. 2012. Effects of cinnamon leaf, oregano and sweet orange essential oils on fermentation and aerobic stability of barley silage. Journal of Science Food Agriculture 92:906-915.
• Covan MM. 1999. Plant products as antimicrobial agents. Clinal Microbiology Reviews. 12:564-582.
• Davidson T R, Stevenson KR. 1973. Influence of formic acid and formalin on quality of direct-cut alfalfa silage. Canadian Journal of Plant Science 3:75-79.
• Ding W, Guo X, Ataku K.2013.Characterization of peptides in ensiled alfalfa treated with different chemical additives. Animal Science Journal. 84:774-781.
• Efe E, Bek Y, Şahin M. 2000. SPSS’te çözümleri ile istatistik yöntemler II. Kahramanmaraş Sütçü İmam Üniversitesi, Yayın No:73, Ders Kitabı Yayın No:9.
• García CC, Talarico L, Almeida N, Colombres S, Duschatzky C, Damonte EB.2003. Virucidal activity of essential oils from aromatic plants of San Luis, Argentina. Phytotherapy Research.17:1073-1075.
• Henderson N. 1993. Silage additives. Animal Feed Science and Technology. 45:35-56.
• Janssen A M, Scheffer J J C, Baerheim Svendsen A. 1987. Antimicrobial activity of essential oils: a 1976-1986 literature review. Aspects of the test methods. Planta Medica. 53:395-398.
• Jeroch H, Drochner W, Simon O. 1999. Nutrition on farm livestock. Verlag Eugen Ulmer Stuttgart. 525 p.
• Kamalak A. 2005. Bazı kaba yemlerin gaz üretim parametreleri ve metabolik enerji içerikleri bakımından karşılaştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Fen ve Mühendislik Dergisi. 8(2):116-120.
• Kung, L. Jr., Tung, S., Maciorowski, K.G., Buffum, K., Knutsen, K., Aimutis, W.R., 1991. Effect of plant cell-wall-degrading enzymes and lactic acid bacteria on silage fermentation and composition. J. Dairy Sci. 74, 4284–4296.
• Kung JL, Williams P, Schmidt RJ, Hu W. 2008. A blend of essential plant oils used as an additive to alter silage fermentation or used as a feed additive for lactating dairy cows. Journal of Dairy Science 91:4793-4800.
• Kurtoğlu V. 2011. Silaj ve silaj katkıları. Aybil Yayınevi, Konya.
• Matan N, Rimkeeree H, Mawson AJ, Chompreeda P, Haruthaithansan V, Parker M. 2006. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. International Journal of Food Microbiology.107:180-185.
• McAllister, T.A., Feniuk,R., Mir, Z., Mir, P., Selinger, L.B., Cheng, K.-J. 1998. Inoculants for alfalfa silage: Effects on aerobic stability, digestibility and the growth performance of feedlot steers. Livestock Prod. Sci. 53:171-18.
• McDonald P, Henderson N, Heron S. 1991. The biochemistry of silage. 2nd ed. Chalcombe publications, Marlow, Bucks, UK. 340 pp..
• Naumann C, Bassler R. 1993. Methoden Buch, B. III. Die chemische untersuchung von futtermitteln. VDLUFA- Verlag, Darmstadt.
• Reichling J, Schnitzler P, Suschke U, Saller R. 2009. Essential oils of aromatic plants with antibacterial, antifungal, antiviral, and cytotoxic properties-An Overview. Forschende Komplementärmedizin. 16:79-90.
• Rooke JA, Hatfield RD. 2003. Biochemistry of ensiling. In: Buxton DR , Muck RE , Harrison JH (eds), Silage Science and Technology, p. 133. ASA‐CSSA‐SSSA, Madison, WI.
• Salawu M B, Acamovic T, Stewart C S, Hvelplund T, Weisbjerg M R. 1999. The use of tannins as silage additives: effects on silage composition and mobile bag disappearance of dry matter and protein. Anim. Feed Sci. Technol. 82: 243–259.
• Seale DR, Pahlow G, Spoelstra SF, Lindgren S, Dellaglio F, Lowe JF. 1990. Methods forthe microbiological analysis of silage. Proceeding of the Eurobac Conference.147. Uppsala.
• Soycan-Önenç S, Akkan S .2008. Bazı aromatik bitkilerin rumen uçucu yağ asitleri üzerine etkileri. V. Ulusal Hayvan Besleme Kongresi, 30 Eylül-03 Ekim, Çorlu/Tekirdağ.
• Soycan-Önenç S, Koç F, Coşkuntuna L, Özdüven ML, Gümüş T. 2015. The effect of oregano and cinnamon essential oils on fermentation quality and aerobic stability of field pea silages. Asian-Australasian Journal of Animal Science 28(9):1281–1287.
• Soycan-Önenç S, Coşkuntuna L, Koç F, Özdüven ML, Gümüş T. 2017. Effects of essential oils of oregano and cinnamon on fermentation quality and ın vitro metabolic energy of field pea silages. Animal Production 58(2):39-44.
• Sukutta U, Haruthaithansan V, Chantarapanont W, Dilokkunanant U, Suppakul P. 2008. Antifungal activity of clove and cinnamon oil and their synergistic aganist postharvest decay fungi of grape in vitro. Kasetsart Journal-Natural Science. 42:169-174.
• SPSS Inc., 2009. PASW Statistics for Windows, Version 18.0, SPSS Inc.
• TSE, 1991. Hayvan yemleri- metabolik (çevrilebilir) enerji tayini (kimyasal metod). TS 9610, Aralık 1991, Ankara.
• Thompson D P. 1989. Fungitoxic activity of essential oil components on food storage fungi. Mycologia. 81(1):151-153.
• Tremblay G F, Bélanger G, McRae K B, Michaud R. 2001. Proteolysis in alfalfa silages made from different cultivars.Canadian Journal of Plant Science 81(4): 685-692.
• Turan A, Soycan Önenç S. 2018. Effect of cumin essential oil usage on fermentation quality, aerobic stability and in vitro digestibility of alfalfa silage. Asian-Australasian Journal of Animal Science. 31(8):1252-1258.
• Wilkinson J M, Davies D R. 2013. The aerobic stability of silage:Key findings and recent developments. Grass and Forage Science.68:1-19.
• Woolford M K, Cook J E. 1978. A note on the effects on the aerobic deterioration of maize silage of the manipulation of the microflora by means of antibiotics. Animal Feed Science and Technology 3:89–94.