IGF-1/CaC8I, LEP/NmuCI and PRLR/Hin1II Polymorphisms in Saanen Goats

Yıl 2022, Cilt: 63 Sayı: 1, 15 - 20, 01.07.2022

Mustafa Can Yılmaz , Prof.dr.güldehen Bilgen

https://doi.org/10.29185/hayuretim.1018577

Öz

Objective: This study was aimed to identify IGF-1, LEP and PRLR polymorphisms by PCR-RFLP method in Saanen goats.
Material and Methods: In the study, nasal samples were collected from 72 Saanen goats via swabs and DNA was extracted. Polymorphisms of IGF-1, LEP and PRLR were determined by PCR amplification followed by RFLP method using restriction enzymes Cac8I, NmuCI and Hin1II, respectively.
Results: LEP/NmuCI produced three genotypes as TT, TC and CC with frequencies 90.3, 6.9 and 2.8%, respectively and two alleles (T=93.8%, C=6.2%). The frequencies of genotype for PRLR gene were CC (90.3%), CT (5.5%) and TT (4.2%) and allele frequencies were C (93.1%) and T (6.9%). The IGF-1/Cac8I locus was monomorphic in Saanen goat population.
Conclusion: The present results may be useful for future studies conducted on goat genome with respect to IGF-1, LEP and PRLR genes.

Anahtar Kelimeler

DNA markers, PCR-RFLP, single nucleotide polymorphism, nasal swab

Destekleyen Kurum

Ege Üniversitesi ÖYP Birimi

Teşekkür

This paper is a part of the PhD thesis of Mustafa Can YILMAZ who was supported by Faculty Member Training Program (ÖYP).

Saanen Keçilerinde IGF-1/Cac8I, LEP/NmuCI ve PRLR/Hin1II Polimorfizmleri

Öz

Amaç: Bu çalışmada Saanen keçilerinde IGF-1, LEP ve PRLR polimorfizmlerinin PCR-RFLP yöntemi ile saptanması amaçlanmıştır.
Materyal ve Yöntem: Çalışmada, 72 Saanen keçisinden sürüntü yoluyla burun içi epitel hücre örnekleri toplanmış ve DNA izolasyonu yapılmıştır. IGF-1, LEP ve PRLR polimorfizmleri, sırasıyla Cac8I, NmuCI ve Hin1II restriksiyon enzimleri kullanılarak PCR amplifikasyonunu takiben RFLP yöntemi ile belirlenmiştir.
Bulgular: LEP/NmuCI lokusunda genotip frekansları TT (%90.3), TC (%6.9) ve CC (%2.8) ve allel frekansları T (%93.8), C (%6.2) şeklinde tespit edilmiştir. PRLR/Hin1II için genotip frekansları CC (%90.3), CT (%5.5), CC (%4.2) ve allel frekansları C (%93.1) ve T (%6.9) olarak hesaplanmıştır. IGF-1/Cac8I lokusunun popülasyonumuzda monomorfik olduğu tespit edilmiştir.
Sonuç: Bu sonuçların IGF-1, LEP ve PRLR genleri ile ilgili olarak keçi genomu üzerinde yapılacak gelecekteki çalışmalar için faydalı olabileceği düşünülmektedir.

Anahtar Kelimeler

DNA belirteçleri, PCR-RFLP, tek nükleotid polimorfizmi, nazal sürüntü

Kaynakça

• Abdalhag MA, Li T, Duan L, Zhang T, Zhang G, Wang. J. 2016. Association analysis of IGF-I gene expression with growth and reproductive traits in Jinghai yellow chickens. Genetics and Molecular Research 15: 1-11.
• An XP, Hou JX, Gao TY, Lei YN, Li G, Song YX, Wang JG, Cao BY. 2015. Single-nucleotide polymorphisms g. 151435C > T and g. 173057T > C in PRLR gene regulated by bta-miR-302a are associated with litter size in goats. Theriogenology 83: 1477-1483.
• Anton I, Kovács K, Holló G, Farkas V, Lehel L, Hajda Z, Zsolnai A. 2011. Effect of leptin, DGAT1 and TG gene polymorphisms on the intramuscular fat of Angus cattle in Hungary. Livestock Science 135: 300-30.
• Anton I, Kovács K, Holló G, Farkas V, Szabó F, Egerszegi I, Rátky J, Zsolnai A, Brüssow KP. 2012. Effect of DGAT1, Leptin and TG gene polymorphisms on some milk production traits in different dairy cattle breeds in Hungary. Archiv fur Tierzucht 55: 307-314.
• Baran N, Kelly PA, Binart N. 2002. Characterization of a prolactin-regulated gene in repro ductive tissues using the prolactin receptor knockout mouse model. Biology of Reproduction 66: 1210-1218.
• Bayram D, Akyüz B, Arslan K, Özdemir F, Aksel EG, Çınar MU. 2019. DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambs and their effects on live weights up to weaning age. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25:9-15.
• Boucher D, Palin MF, Castonguay F, Gariépy C, Pothier F. 2006. Detection of polymorphisms in the ovine leptin (LEP) gene: Association of a single nucleotide polymorphism with muscle growth and meat quality traits. The Canadian Journal Animal Science 86: 31-35.
• Cosenza G, Iannaccone M, Auzino B, Macciotta NPP, Kovitvadhi A, Nicolae I, Pauciullo A. 2018. Remarkable genetic diversity detected at river buffalo prolactin receptor (PRLR) gene and association studies with milk fatty acid composition. Animal Genetics 49: 159-168.
• Curi RA, Chardulo LAL, Arrigoni MDB, Silveira AC, de Oliveira HN. 2011. Associations between LEP. DGAT1 and FABP4 gene polymorphisms and carcass and meat traits in Nelore and crossbred beef cattle. Livestock Science 135: 244-250.
• Daş A, Şahin T, Akbulut Ö, Bengü AŞ, Bozkaya F. 2019. Türkiye’de yetiştirilen et ırkı kültür sığırlarında leptin. ghrelin ve insülin benzeri büyüme faktörü-1(IGF-1) gen polimorfizmlerinin belirlenmesi. Harran Üniversitesi Veteriner Fakültesi Dergisi 8 (1): 108-115.
• Darwish HR, El-Shorbagy HM, Abou-Eisha AM, El-Din AE, Farag IM. 2017. New polymorphism in the 5’flanking region of IGF-1 gene and its association with wool traits in Egyptian Barki sheep. Journal of Genetic Engineering and Biotechnology 15: 437-41.
• de Oliveira JA, da Cunha CM, Crispim Bdo A, Seno L, de O, Fernandes AR, NogueiraGde P, Grisolia AB. 2013. Association of the leptin gene with carcass characteristicsin Nellore cattle. Animal Biotechnology 24: 229-242.
• Dettori ML, Pazzola M, Paschino P, Amills M, Vacca GM. 2018. Association between the GHR. GHRHR. and IGF1 gene polymorphisms and milk yield and quality traits in Sarda sheep. Journal of Dairy Science 101(11): 9978-9986.
• Di R, Yin J, Chu MX, Cao GL, Feng T, Fang L, Zhou ZX. 2011. DNA polymorphism of introns 1 and 2 of Prolactin Receptor Gene and its association with litter size in goats. Animal Science Papers and Reports 29: 343-350.
• Esmaeili NAV, Esmaeilizadeh AK, Ayatolahi AM. 2020. Genetic diversity of leptin gene intron 2 in wild and native and exotic goat breeds in Iran. Journal of Microbiology, Biotechnology and Food Sciences 9: 1110-1113.
• Food and Agriculture Organization of the United Nations (FAO) Food and Agriculture Organization of the United Nations statistical databases. http://faostat.fao.org/ (20 Ekim 2021)
• Gui LS, Wang ZY, Jia JL, Zhang CT, Chen YZ, Hou SZ. 2018. IGF-1 gene polymorphisms influence bovine growth traits in Chinese Qinchuan cattle. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 24(3).
• Hou JX, An XP, Song YX, Wang JG, Ma T, Han P, Fang F, Cao BY. 2013. Combined effects of four SNPs within goat PRLR gene on milk production traits. Gene 529: 276–281.
• Hou JX, Fang F, An XP, Yan Y, Ma T, Han P, Meng FX, Song YX, Wang JG, Cao BY. 2014. Polymorphisms of PRLR and FOLR1 genes and association with milk production traits in goats. Genetics and Molecular Research 13: 2555-2562.
• Işık, R, Bilgen G. 2019. Associations between genetic variants of the POU1F1 gene and production traits in Saanen goats, Archives of Animal Breeding 62; 249–255.
• Işık R, Özdil F. 2020. Leptin receptor gene polymorphisms in some Turkish donkey populations, Journal of Equine Veterınary Science, 84: 102823-102826.
• Kawaguchi F, Okura K, Oyama K, Mannen H, Sasazaki S. 2017. Identification of leptin gene polymorphisms associated with carcass traits and fatty acid composition in Japanese Black cattle. Animal Science Journal 88: 433-438.
• Kök S, Vapur G. 2021. Effects of leptin and thyroglobulin gene polymorphisms on beef quality in Holstein breed bulls in Turkey. Turkish Journal of Veterinary and Animal Sciences 45: 238-247.
• Kulig H, Kmiec M. 2009. Association between leptin gene polymorphisms and growth traits in Limousin cattle. Genetika. 45: 838-41.
• Leifers SC, Veekamp RF, Te Pas MF, Delvand C, Chiliard Y, Vander Lende T. 2003. Leptin concentration in relation to energy balance, milk yield, intake, live weight and estrus in dairy cattle. Journal of Dairy Science. 86: 799-807.
• Li G, An XP, Fu MZ, Hou JX, Sun RP, Zhu GQ, Wang JG, Cao BY. 2011. Polymorphism of PRLR and LHβ genes by SSCP marker and their association with litter size in Boer goats. Livestock Science 136: 281-286.
• Li HF, Zhu WQ, Chen KW, Wu X, Tang. Q, Gao Y. 2008. Associations between GHR and IGF-1 gene polymorphisms and reproductive traits in Wenchang chickens. Turkish Journal of Veterinary and Animal Sciences 32: 281-285.
• Liang K, Wang X, Tian X, Geng R, Li W, Jing Z, Han R, Tian Y, Liu X, Kang X, Li Z. 2019. Molecular characterization and an 80-bp indel polymorphism within the prolactin receptor (PRLR) gene and its associations with chicken growth and carcass traits. 3 Biotech 9(8): 296.
• Lu A, Hu X, Chen H, Dong Y, Pang Y. 2011. Single nucleotide polymorphisms of the prolactin receptor (PRLR) gene and its association with growth traits in Chinese cattle. Molecular Biology Reports 38: 261-266.
• Mullen MP, Berry DP, Howard DJ, Diskin MG, Lynch CO, Giblin L, Kenny DA, Magee DA, Meade KG, Waters SM, David A. 2011. Single nucleotide polymorphisms in the insulin-like growth factor 1 (IGF-1) gene are associated with performance in Holstein-Friesian dairy cattle. Frontiers in Genetics 2: 1-9.
• Naicy T, Venkatachalapathy T, Aravindakshan T, Raghavan KC, Mini M, Shyama K. 2017. Association of a novel single nucleotide polymorphism at the exon-2 of Insulin-Like Growth Factor 1 (IGF 1) gene with phenotypic variants in goats. Veterinarski arhiv 87: 457-472.
• Saleem AH, Javed K, Babar ME, Hussain T, Ali A, Ahmad N, Farooq MZ and Dawood M. 2018. Association of Leptin Gene Polymorphism with Growth Rate in Lohi Sheep. Pakistan Journal of Zoology. 50(3): 1029-1033.
• Shanaz S, Ganai TAS, Hussain I, Ahmad SF, Sheikh FD, Shabir N, Nabi N, Ganai NA. 2020. IGF-1 gene polymorphism and its association with cashmere fiber trait in Changthangi goats. Journal of Natural Fibers 17(12): 1819-1826.
• Shareef M, Basheer A, Zahoor I, Anjum AA. 2018. Polymorphisms in growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene and their association with growth traits in beetal goat. Pak. Journal of Agricultural Sciences 55(3): 713-720.
• Shojaei M, Mohammad Abadi M, Asadi Fozi M, Dayani O, Khezri A, Akhondi M. 2010. Association of growth trait and Leptin gene polymorphism in Kermani sheep. Journal of Cell and Molecular Research 2: 67-73.
• Supakorn C and Pralomkarn W. 2013. Genetic polymorphisms of growth hormone (GH). insulin-like growth factor 1 (IGF-1) and diacylglycerol acyltransferase 2 (DGAT–2) genes and their effect on birth weight and weaning weight in goats. Philipp Agriculture Scientist 96: 18-25.
• Tölü C, Yurtman İY, Savaş T. 2010. Gökçeada. Malta ve Türk Saanen Keçi Genotiplerinin Süt Verim Özellikleri Bakımından Karşılaştırılması. Hayvansal Üretim 51(1): 8-15.
• Trakovická A, Moravčíková N, Kasarda R. 2013. Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochimica Polonica 60: 783-787.
• Wang C, Zhang H, Niu L, Guo J, Jia X, Wang L, Li L, Zhang H, Zhong T. 2015. The novel SNPs of leptin gene and their associations with growth traits in Chinese Nanjiang Yellow goat. Gene 572: 35-41.
• Werner H, Bruchim I. 2009. The insulin-like growth factor-I receptor as an oncogene. Archives of Physiology and Biochemistry 115:58-71.
• Xiong Q, Chai J, Li XF, Suo XJ, Zhang N, Tao H, Liu Y, Yang QP, Jiang SW, Chen MX. 2016. Two tagSNPs in the prolactin receptor gene are associated with growth and litter traits in Boer and Macheng Black crossbred goats. Livestock Science 193:71–77.
• Yeh F, Boyle T. 1999. POPGENE version 1.3.2: Microsoft window-based freeware for population genetic analysis.
• Yoshimura Y. 1998. Insulin-like Growth Factors and Ovarian Physiology. Journal of Obstetrics and Gynaecology Research 24(5): 305-323.
• Younis LS,, Al-Mutar HAA, Abid AA. 2019. Effect of leptin gene polymorphism on reproductive efficiency in awassi ewes. Advances in Animal and Veterinary Sciences 7(1): 17-23.
• Yu LM, Jiang ML, Qiang SG, Jie PQ, Wei S, Lian WG. 2004. Polymorphism analysis of goat growth hormone gene in the 5’ regulatory sequence. Hereditas 6: 831-5.
• Zhou JP, Zhu XP, Zhang W, Qin F, Zhang SW, Jia ZH. 2011. A novel single-nucleotide polymorphism in the 5’ upstream region of the prolactin receptor gene is associated with fiber traits in Liaoning cashmere goats. Genetics and Molecular Research 10: 2511-2516.