Associations of VLDLR Gene Polymorphism (intron 11, 392C > T) with Egg Production and Weight in Japanese Quails (Coturnix coturnix japonica)

Yıl 2025, Cilt: 66 Sayı: 1, 25 - 31, 30.06.2025

Halime Karaköse , Eymen Demir , Ümit Bilginer , Veli Atmaca , Bahar Argun Karslı , Taki Karslı

https://doi.org/10.29185/hayuretim.1607156

Öz

Objective: This study aimed to assess the association between the VLDRLR gene intron 11 (392 C>T) polymorphism and egg production and weight in quails.
Material and Methods: Egg yield and weight were recorded over 90 days for 191 Japanese quails, which were genotyped using the restriction fragment length polymorphism technique for the VLDLR gene variation.
Results: The VLDLR gene was polymorphic due to conserving all possible genotypes (TT, TC, and CC). The TT genotype was the most common with a frequency of 0.70, while the frequency of the TC and CC genotype were 0.16 and 0.14, respectively. The mean 90-day egg production was 78.31, 76.07, and 73.94 in TT, TC, and CC genotypes, respectively, while the mean egg weight ranged from 915.80 (CC genotype) to 939.19 (TT genotype). Association analysis revealed a significant relationship between the VLDLR genotypes and egg production traits (P<0.05), while no significant relationship was detected for egg weight.
Conclusion: This study showed that the TT genotype for VLDLR gene intron 11 (392 C>T) polymorphism can be used in marker-assisted selection studies in order to increase egg production in quails.
Keywords: Candidate genes, egg traits, genetic variation, MAS, PCR-RFLP

Anahtar Kelimeler

candidate genes, egg traits, genetic variation, MAS, PCR-RFLP

Etik Beyan

No ethical permission was required since DNA was extracted from shed feather samples of animals kept in individual cages.

Japon Bıldırcınlarında (Coturnix coturnix japonica) VLDLR Gen Polimorfizminin (11 intron, 392C > T) Yumurta Üretimi ve Ağırlığı ile İlişkisi

Öz

Amaç: Bu çalışma, Japon bıldırcınlarında VLDLR geninin intron 11 (392 C>T) polimorfizmi ile yumurta üretimi ve ağırlığı arasındaki ilişkiyi değerlendirmeyi amaçlamıştır
Materyal ve Metot: VLDLR gen varyaysyonu için resatriksiyon fragment uzuluğu polimorfizmi tekniğiyle gengenotiplendirilen 191 Japon bıldırcınında 90 günlük yumurta verimi ve ağırlığı verisi kaydedilmiştir.
Bulgular: Muhmetel bütün genotipleri (TT, TC ve CC) içerdiğinden dolayı VLDLR geninin polimorfik olduğu belirlenmiştir. En yaygın genotipin 0.70 frekans ile TT olduğu, TC ve CC genotip frekanslarının ise sırasıyla 0.16 ve 0.14 olduğu belirlenmiştir. 90 günlük yumurta üretimi ortalamasının TT, TC ve CC genotipleri için sırasıyla 78.31, 76.07 ve 73.94 olduğu, yumurta ağırlığı ortalamasının ise 915.80 (CC genotipi) ile 939.19 (TT genotipi) aralığında değiştiği gözlemlenmiştir. Yapılan ilişki analizi, VLDLR genotipleriyle yumurta üreteimi arasında önemli bir ilişkinin olduğunu (P<0.05), yurmurta ağırlığı açısından ise herhangi bir ilişkinin olmadığını göstermiştir.
Sonuç: Bu çalışma, VLDLR geninin intron 11 (392 C>T) polimorfizmini için TT genotipinin japon bldırcınlarında yumurta üretimini arttırmak için uygulanacak olan marker destekli seleksiyon çalışmalarında kullanılabileceğini göstermiştir.
Anahtar sözcükler: Aday genler, yumurta özellikleri, genetik çeşitlilik, MDS, PZR-RFLP

Anahtar Kelimeler

aday genler, yumurta özellikleri, genetik çeşitlilik, MDS, PZR-RFLP

Kaynakça

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