Koyun Kalbinde Yeni Fiksatiflerin ATR-FTIR Spektroskopik Analizi ve Histolojik Özellikleri

Yıl 2024, , 98 - 107, 30.06.2024

Fikri Özdemir , Sevil Özkınalı , Emine Nazlı Hayırlı Özyol

https://doi.org/10.52827/hititmedj.1370056

Öz

Amaç: Bu çalışmada, hücresel yapının ve doku mimarisinin canlı gibi korunması amacıyla, kokusuz ve
toksik olmayan yeni fiksatif çözeltiler koyun kalbi üzerinde test edildi.
Gereç ve Yöntem: Yeni fiksatiflerin dokuyla temas öncesi ve sonrasındaki değişiklikleri ilk kez ATRFTIR
spektroskopik yöntemiyle tespit edildi. Ön incelemenin ardından kalpler altı farklı çözeltiyle
fikse edildi. Ağırlık, boyut, renk ve sertlik açısından periyodik olarak değerlendirildi. Her kalbin sol
ventrikülündenalınan ve Hematoksilen Eozin ile boyanan örnekler histolojik olarak değerlendirildi.
Bulgular: Kalp ağırlıkları ölçümleri sonucunda Çözelti 1 (Standart %10’ luk Formaldehit Çözeltisi),
Çözelti 4 ve Çözelti 5’te sırasıyla %5,64, %23,70 ve %14,38 azalma tespit edildi. Çözelti 4 ve Çözelti 5’te
miyokardın morfolojik açıdan bütünlüğünün korunduğu, sertliğin kesit almaya uygun olduğu ve Çözelti
1’e göre koroner damarların iyi korunduğu gözlendi. Ayrıca Çözelti 1 ile karşılaştırıldığında, Çözelti 4 ve
Çözelti 5’te fikse edilen dokularda endokardiyum ve miyokard katmanlarının iyi korunduğu belirlendi.
Çözelti 1, Çözelti 4 ve Çözelti 5’in içerisindeki kalp kası dokularında tipik enine çizgilenme görünümü
mevcuttu.
Sonuç: Çözelti 4 ve Çözelti 5’in Çözelti 1’e (Formaldehit) göre daha üstün fiksatif özelliklere sahip
olduğu bulundu. ATR-FTIR çalışmaları sonucunda Standart Çözelti 1’de formaldehitin toksik formik asite
dönüştüğü belirlenirken, Çözelti 4 ve Çözelti 5’te çok az ve zararsız değişiklikler tespit edildi.

Anahtar Kelimeler

Anatomi eğitimi, ATR-FTIR spektroskopisi, fiksatif, organ koruma

ATR-FTIR Spectroscopic Analysis of Novel Fixatives and Their Histological Properties on Sheep Heart

Öz

Objective: In this study, new fixative solutions that are odorless and non-toxic were tested on the hearts
of sheep to preserve the cellular structure and tissue architecture in a life-like manner.
Material and Method: The changes of new fixatives before and after contact with tissue were detected
for the first time by the ATR-FTIR spectroscopic method. After a preliminary inspection, hearts were fixed
with six different solutions. It was periodically evaluated for weight, size, color, and hardness. Samples
taken from the left ventricle of each heart stained with Hematoxylin eosin and examined histologically.
Results: As a result of the measurements of heart weights, reduction in Solution 1 (Standard Formaldehyde
Fixative), Solution 4, and Solution 5 were detected at 5.64%, 23.70%, and 14.38%, respectively. In
Solution 4 and Solution 5, it was observed that the integrity of the myocardium was protected in
terms of morphology, the stiffness was suited for sectioning and the coronary vessels were preserved
better than Solution 1. Further, compared to solution 1, it was determined that the endocardium and
myocardium layers were well preserved in the tissues fixed in Solution 4 and Solution 5. Typical cross
striation appearance in cardiac muscle tissue existed in all three Solution 1, Solution 4, and Solution 5.
Conclusion: Solution 4 and Solution 5 were found to have superior fixative properties than Solution 1
(Formaldehyde). As a result of ATR-FTIR studies, it was determined that formaldehyde was converted
into toxic formic acid in Standard Solution 1, while very few harmless changes were detected in Solution
4 and Solution 5.

Anahtar Kelimeler

Anatomy education, ATR-FTIR spectroscopy, fixative, organ preservation

Kaynakça

• Ten Brinke B, Klitsie PJ, Timman R, et al. Anatomy Education and Classroom Versus Laparoscopic Dissection-Based Training: A Randomized Study at One Medical School. Academic Medicine 2014;89(5):806–810.
• Ghosh SK. Cadaveric dissection as an educational tool for anatomical sciences in the 21st century. Anatomical Sciences Education 2017;10(3):286–299.
• Franchi T. The Impact of the Covid-19 Pandemic on Current Anatomy Education and Future Careers: A Student’s Perspective. Anatomical Sciences Education 2020;13(3):312–315.
• Boduç E, Allahverdi T. Student Opinions on the Importance and Detail of the Accumulation of Anatomy Knowledge Integrating General Surgery. Middle Black Sea Journal of Health Science 2020;6:385–389.
• Williams AD, Greenwald EE, Soricelli RL, DePace DM. Medical students’ reactions to anatomic dissection and the phenomenon of cadaver naming. Anatomical Sciences Education 2014;7(3):169–180.
• Memon I. Cadaver Dissection is Obsolete in Medical Training A Misinterpreted Notion. Medical Principles and Practice 2018;27(3):201–210.
• Aziz MA, Mckenzie JC, Wilson JS, Cowie RJ, Ayeni SA, Dunn BK. The human cadaver in the age of biomedical informatics. Anatomical Record 2002;269(1):20–32.
• Estai M, Bunt S. Best teaching practices in anatomy education: A critical review. Annals of Anatomy 2016;208:151–157.
• Ninh LN, Tangkawattana S, Sukon P, Takahashi N, Takehana K, Tangkawattana P. Neutralizing formaldehyde in chicken cadaver with urea and urea fertilizer solution. Journal of Veterinary Medical Science 2018;80(4):606–610.
• Bamisi OD, Alese MO. Effects of various fixatives and temperature on the quality of glycogen demonstration in the brain and liver tissues. Ann Diagn Pathol 2020; 48:151604.
• Singh H, Bishen KA, Garg D, Sukhija H, Sharma D, Tomar U. Fixation and Fixatives: Roles and Functions-A Short Review. Dental Journal of Advance Studies 2019;07(02):51–55.
• Kazarian SG, Chan KLA. Applications of ATR-FTIR spectroscopic imaging to biomedical samples. Biochimica et Biophysica Acta - Biomembranes 2006;1758(7):858–867.
• Li C, Fan H, Sun Y, Tian W, Ma G. Toxicity of aristolochic acid in a rat model investigated by Fourier transform infrared spectroscopy combined with chemometric analysis: An exploratory study. Vibrational Spectroscopy 2021;117:103307.
• Baran MF, Açay H. Antimicrobial Activity Of Silver Nanoparticles Synthesized with Extract of Tomato Plant Against Bacterial and Fungal Pathogens. Middle Black Sea Journal of Health Science 2019;5:67–73.
• Öztürkler Y, Baran A, Evecen M, Ak K, Ileri IK. Comparison of Ovine Spermatozoal Morphological Features after Staining of Fixation and Assessment of Morphological Abnormalities in Dead/Live Spermatozoa. Turkish Journal of Veterinary and Animal Sciences 2001;25(5):675–680.
• Brenner E. Human body preservation-old and new techniques. Journal of Anatomy 2014;224(3):316–344.
• Mayer R.G. Embalming: History, Theory, and Practice. Fifth Edition. New York: Mac Graw Hill Medical Publishing, 2012; 143-210.
• Richins CA, Roberts EC, Zeilmann JA. Improved fluids for anatomical embalming and storage. The Anatomical Record 1963;146(3):241–243.
• Bradbury SA, Hoshino K. An improved embalming procedure for long-lasting preservation of the cadaver for anatomical study. Cells Tissue Organs 1978;101(2):97–103.
• Björkman N, Nielsen P, Hornshøj Møller V. Removing formaldehyde from embalmed cadavers by percolating the body cavities with dilute ethanol. Acta anatomica 1986;126(2):78–83.
• Hammer N, Löffler S, Feja C, et al. Ethanol-glycerin fixation with thymol conservation: A potential alternative to formaldehyde and phenol embalming. Anatomical Sciences Education 2012;5(4):225–233.
• Woodburne RT, Lawrence CA. An improved embalming fluid formula. The Anatomical record 1952;114(3):507–514.
• Scholz-Böttcher BM, Nissenbaum A, Rullkötter J. An 18th century medication “Mumia vera aegyptica” - Fake or authentic? Organic Geochemistry 2013;65:1–18.
• Gibb T.J. Insect Collection and Identification. Second Edition. Cambridge: Academic Press; 2020; 73-124.
• Yamanaka T, Bannai H, Tsujimura K, Nemoto M, Kondo T, Matsumura T. Comparison of the virucidal effects of disinfectant agents against equine influenza a virus. Journal of Equine Veterinary Science 2014;34(5):715–718.
• Silva RMG, Matera JM, Ribeiro AACM. New alternative methods to teach surgical techniques to veterinary medicine students despite the absence of living animals. Is that an academic paradox? Anatomia, histologia, embryologia 2007;36(3):220–224.
• O’Sullivan E, Mitchell BS. An improved composition for embalming fluid to preserve cadavers for anatomy teaching in the United Kingdom. Journal of Anatomy 1993;182(2):295–297.
• Rohrig TP. Embalming Artifacts. Postmortem Toxicology 2019;123–139.