Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents

Abstract

Artichoke leaf (Cynara Scolymus) extracts widely used in medicine. Chlorogenic Acids have antioxidant, antibacterial and antiviral properties is an compound.

In this study, after the artichoke (Cynara scolymus) leaves had  dried, powdered portions were extracted to with water. The results had expressed as ppm after  extracts  were analyzed by HPLC. It were determined that optimum conditions of extracting chlorogenic acid from Cynara Scolymus leaves with the ultrasonic assisted extraction with Response Surface Method (RSM) based on Box-Behnken design. The experimental conditions for this extraction efficiency were determined as extraction time (15-45 minutes), temperature (30-70⁰C) and ultrasound power (25-75%).  The optimum conditions were determined as 47.65% ultrasound power, 35.18 minutes, 53.40⁰ C temperature. The maximum yield of chlorogenic acid was determined to 10.05 ppm.

Also the total phenolic content was measured by UV -Vis spectrometry in order to see antioxidant activity.

Absorbance values of samples which are took from each sample  were measured at 765 nm in UV spectrometer. Optimum conditions were determined as 47.29% ultrasound power, 29.67 minutes, 55.33% ethanol concentration. Total phenolic content were determined as 70.24 ppm. According the to results ultrasonic-assisted extraction (UAE) showed can be an effective method for the extraction of chlorogenic acid active ingredient.

Keywords

• Chlorogenic Acid,Artichoke leaf (Cynara scolymus),Extraction,Optimization,RSM

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