Modified Graphite Surfaces Prepared for Electrochemical Biomolecular Interaction Detection Studies

Abstract

Bu çalışma, çift sarmal DNA (dsDNA) ile önemli ve sık kullanılan bir antikanser ilacı olan Mitomisin C (MMC) arasındaki biyomoleküler etkileşimin görüntülenmesi için elektroaktif polimer modifiye elektrot malzemelerinin hazırlanmasını göstermektedir. Modifiye elektrot malzemeleri, o-fenilendiamin (oPD) monomerinin nanomalzeme içeren bir çözeltide elektropolimerizasyonu ile oluşturulmuştur. Katkı maddesi (dopant) molekül olarak kullanılan nanomalzeme grafen (GN)’dir ve elektropolimerizasyon tekniği dönüşümlü voltametri (CV)’dir. Sonrasında poli(o-fenilendiamin) polimer modifiye yüzeylere dsDNA immobilizasyonu gerçekleştirilmiştir. Oluşturulan nanomalzeme katılmış bu polimer modifiye elektrotlar, dsDNA-MMC etkileşiminin tespitinde biyotayin platformları olarak kullanılmışlardır. Biyomoleküler etkileşimlerini aydınlatmak için farklı MMC etkileşim süreleri çalışılmıştır.

Keywords

• Modified electrodes,Electropolymerization,dsDNA,Mitomycin C

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