A Bacterial Machinery for Surface Displayed Enzymes

Yıl 2018, Cilt: 46 Sayı: 2, 307 - 313, 03.06.2018

Urartu Özgür Şafak Şeker

Öz

Biomaterial based protein delivery systems have been utilized for many applications in biomedicine. Despite
their great success, there is a need to develop innovative living, decision making systems for protein
delivery. In this context, here, a cellular system is proposed for protein release and delivery. Such systems can
be used not for biomedical purposes but also for other biochemical applications. In this regard a Escherichia
coli autotransporter protein, Ag43 was engineered to display on its cell membrane. Using this system alkaline
phosphatase protein is displayed on the cell surface as a fusion of Ag43-ALP which is also carrying a specific
TEV protease excision site. It was shown that the active from of the enzyme was released upon its interaction
with TEV protease from the cell surface. In this study a cellular machinery is proposed to be used as a controlled
enzyme delivery system. 

Anahtar Kelimeler

Whole cell biocatalysis, autotransporter, synthetic biology

Kaynakça

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