A new, sensitive and disposable electrochemical immunosensor based on Benzaldehyde side group containing phosphazene polymer modified ITO substrate for Interleukin 1β detection

Year 2019, Volume: 47 Issue: 3, 305 - 315, 23.10.2019

Muhammet Aydın

https://doi.org/10.15671/hjbc.515999

Cited By: 1

Abstract

Bu çalışmada,
interlökin 1β (IL 1β) tespiti için tek kullanımlık benzaldehit ikameli fosfazen
polimeri (BSPP) ile modifiye edilmiş ITO elektrot temelli yeni bir
elektrokimyasal ultrahassas immünosensör geliştirilmiştir. Aldehit yan grupları
içeren fosfazen polimeri (BSPP) halka açma polimerizasyonu yöntemi ile
sentezlenmiştir. Bu aldehit grupları, anti-IL 1β antikorları için bir bağlantı
noktaları sağlamıştır. Önerilen immünosensörün üretim süreci, Elektrokimyasal Empedans
Spektroskopisi (EIS) ve Döngüsel Voltammetri (CV) gibi elektrokimyasal
tekniklerle izlendi. Ek olarak, bu üretim aşamaları, Taramalı Elektron
Mikroskobu (FE-SEM) ve Atomik Kuvvet Mikroskobu (AFM) kullanılarak karakterize
edildi. Ayrıca, polimer kaplı elektrot yüzeyindeki BSPP polimer tabakası,
Enerji Dağıtıcı X-ışını (EDX) kullanılarak araştırıldı. Üretilen immünosensör
düşük bir tespit sınırına (9,3 fg / mL) ve geniş bir doğrusal tayin aralığına
(0,03-7,5 pg / mL) sahiptir. Ayrıca, iyi tekrarlanabilirlik (% 1.82), mükemmel
tekrarlanabilirlik (% 1.56), iyi seçicilik ve yüksek stabiliteye sahipti. Deney
sonuçları, BSPP polimerinin, klinik teşhis ve pratik uygulamalarda IL1β antijen
tespiti için arzu edilen bir platform olduğunu gösterdi. Önerilen biyosensörün
uygulanabilirliği, insan serumunda IL 1β seviyesi ölçülerek test edildi ve
önerilen immünosensör, nicel analiz için kabul edilebilir sonuçlara sahipti.

Keywords

Poly(phosphazene), Label free immunosensor, Electrochemical impedance spectroscopy

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