The Phenolic Compounds from Hypericum Perforatum and Their Antimicrobial Activities

Yıl 2008, Cilt: 36 Sayı: 4, 339 - 345, 01.12.2008

Gönül Çelen Semiha Özkan Fatma Ayhan

Öz

Hypericum perforatum St. John’s wort H. perforatum is a plant which has been used as a medicinal herb sinceancient times and grow in Europe, West Asia and North Africa. In this work, the crude extracts of HypericumPerforatum grown in Muğla were obtained by the extraction with acetone:water and water. The extracts werechemically investigated for their total phenol contents by the Folin–Ciocalteu method. The phenolic compounds in theextract with acetone:water 7:3, v/v, 150.44 mg GA/g material was determined as almost five fold higher than waterextraction process 33.01 mg GA/g material . The thin layer chromatographic TLC analysis revealed the presenceof polyphenolic compounds such as tannins

Anahtar Kelimeler

Hypericum perforatum, Tannins, Total phenol content, TLC, antimicrobial activity, Muğla

Kaynakça

• 1. Hashida, C., Tanaka, N., Kashıwada, Y., Ogawa, M. and Takaıshı, Y., Prenylated Phloroglucinol Derivatives from Hypericum perforatum var. Angustifolium Chem. Pharm. Bull. 56(8) , 1164-67, 2008.
• 2. Duru, B., Middle East Technical University, Graduate thesis, December, 2003
• 3. Luger, P., Weber, M., Kashino, S., Amakura, Y., Yoshida, T., Okuda, T., Beurskens, G., Dauter, Z., Acta Cryst. B: 54, 687, 1998.
• 4. Hagerman, A. E. Web site: http://miavsx1.muohio.edu/~hagermae/
• 5. Muelley-Harvey, I., Animal Feed Science and Technology, 91, 3-20, 2001.
• 6. Ishiguro, K., Yamaki, M., Kashihara, M., Takagi, S., Sarothralen, A., New antibiotic compounds from Hypericum japonicum, Planta Med. 52, 288-90, 1986.
• 7. Décosterd, L.A., Hoffmann, E., Kyburz, R., Bray, D., Hostettmann, K., A new phloroglucinol derivative from Hypericum calycinum with antifungal and in vitro antimalarial activity. Planta Med. 57, 548-51, 1991.
• 8. Jayasuriya, H., Clark, A.M., McChesney, J.D., New antimicrobial filicinic acid derivatives from Hypericum drummondii, J. Nat. Prod. 54, 1314–1320, 1991.
• 9. Yamaki, M., Ishiguro, K., Antimicrobial activity of naturally ocurring and synthetic phloroglucinol against Staphylococcus aureus, Phytother Res. 8: 112-4, 1994.
• 10. Rocha, L., Marston, A., Potterat, O., Kaplan, M.A.C., Stoeckli- Evans, H., Hostettmann, K., Antibacterial phloroglucinols and flavonoids from Hypericum brasiliense, Phytochemistry 40, 1447-52, 1995.
• 11. Ishiguro, K., Yamaki, M., Kashihara, M., Takagi, S., Soroaspindin, A.B., and C: additional antibiotic compounds from Hypericum japonicum. Planta Med. 53, 415-7, 1987.
• 12. Jayasuriya, H., McChesney, J.D., J. Nat. Prod. 52, 325-31, 1989.
• 13. Décosterd, L., Stoeckli-Evan, H., Msonthi, J.D., Hostettmann, K., A new antifungal chromene and a related dichromene from Hypericum revolutum. Planta Med. 55, 429, 1986.
• 14. Ishiguro, K., Yakamoto, R.,, Oku H., Patulosides, A.B., J Nat. Prod. 62, 113-7, 1999.
• 15. Ishiguro, K., Nagata, S., Fukumoto, H., Yamaki, M., Isoi, K., Oyama, Y., Isopentenylated flavonol from Hypericum japonicum. Phytochemistry 32, 1583-5, 1993.
• 16. Scalbert, A., Antimicrobial properties of tannins, Phytochemistry 30, 3875-83, 1991.
• 17. Dall’Agnol, R., Ferraz, A., Bernardi, A. P., Albring, D., Nör, C., Sarmento, L., La L., Hass, M., Von Poser, G., and Schapoval., E. E. S., Antimicrobial activity of some Hypericum species., Phytomedicine 10, 511-6, 2003.
• 18. Hagerman, A. H., Tannin Chemistry, 2002.
• 19. Mole, P.G., Analysis of Phenolic Plant Metabolites. Blackwell, Oxford. 1994.
• 20. Porter, L.J., Tannins. In: Harborne, J.B., (Ed.), Methods in Plant Biochemistry: Plant Phenolics, Vol.1. (Chapter 11) Academic Press, London, pp. 389-419, 1989.
• 21. Muelley-Harvey, I., Animal Feed Science and Technology, 91, 3-20, 2001.
• 22. Hagerman, A.E.; Butler, L.G., Journal of Biology and Chemistry, 256, 4494–4497, 1981.
• 23. Changez, M., Burugapalli, K., Koul, V., and Choudhary, V., The effect of composition of poly(acrylic acid)-gelatin hydrogel on gentamicin sulphate release: in vitro. Biomaterials 24:527-36, 2003.
• 24. Ayhan, F., Özkan, S., Gentamicin Release from Photopolymerized PEG Diacrylate and pHEMA Hydrogel Discs and Their in Vitro Antimicrobial Activities, Drug Delivery, 14:7, 433 -9, 2007.
• 25. Skerget, M., Kotnik, P., Hadolin, M., Hras, A.R., Simonic, M., Knez, Z., Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities, Food Chemistry, 89, 191–198, 2005.
• 26. Schmitt, A.C., Ravazzolo, A.P., von Poser, G.L., Investigation of some Hypericum species native to Southern of Brazil for antiviral activity, Journal of Ethnopharmacology, 77 (2001) 239–245
• 27. Males, Z., Brantner, A.H., Pilepic, K.H., Plazibat, M., Comparative phytochemical and antimicrobial investigations of Hypericum perforatum L. subsp. perforatum and H. perforatum subsp. angustifolium (DC.) Gaudin Acta Pharmacia, 56, 359-367, 2006.