The Antiproliferative effects of subtituents in formazan derivatives aganist HeLa and C6 cell line

Yıl 2013, Cilt: 41 Sayı: 4, 371 - 378, 01.11.2013

Ayse Şahin Yağlıoğlu Hülya Şenöz

Öz

Antiproliferation activity of TPF and 1-substituted phenyl-3- p-methoxycarbonyl phenyl-5-phenylformazans were determined against HeLa an C6 cell line using BrdU cell proliferataion ELISA assay. Cisplatin and 5-florouracil were used as standarts. The activities of samples and standarts were investigated on eight concentations. The effects of substituents and substituents positions on antiproliferation activities were examined. Assay experiments indicated that o- OCH3, m-CH3 and p-I displayed maximum activity against HeLa while o- NO2, m-I and also p-I were active against C6.

Anahtar Kelimeler

Antiproliferative activity, HeLa, C6, formazan derivatives, substituent effect

Formazan türevlerindeki sübstitüentlerin HeLa ve C6 hücrelerine karşı antiproliferatif etkileri

Öz

T PF ve 1-sübstitüe fenil-3- p-metoksikarbonil fenil-5-fenilformazanların antiproliferatif aktiviteleri HeLa ve C6’ya karşı BrdU hücre proliferasyon ELİSA yöntemi ile belirlendi. Standart olarak sisplatin ve 5-florourasil kullanıldı. Örneklerin ve standartların aktiviteleri sekiz farklı derişimde incelenmiştir. Sübstitüentlerin ve sübstitüent konumlarının antiproliferatif aktivite üzerine etkileri araştırıldı. Deneysel çalışmalar sonucunda, o- OCH3, m-CH3 ve p-I sübstitüentlerinin HeLa hücresine karşı, o- NO2, m-I ve p-I sübstitüentlerinin ise C6 hücresine karşı maksimum etkiye sahip olduğu belirlendi

Anahtar Kelimeler

Antiproliferatif aktivite, HeLa, C6, formazan, sübstitüent etkisi

Kaynakça

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