Satureja boissieri Uçucu Yağının Antimikrobiyal, Antiproliferatif ve Antioksidan Özelliklerinin Araştırılması

Yıl 2017, Cilt: 45 Sayı: 1, 125 - 133, 01.03.2017

Feyza Öke Altuntaş İbrahim Demirtaş

Öz

B u çalışmanın amacı Satureja boisiseri Hausskn. ex Boiss. Lamiaceae uçucu yağının biyolojik aktivitelerinin araştırılmasıdır. Uçucu yağın büyük bileşiklerinin antimikrobiyal aktivitesi agar kuyu ve disk difüzyon yöntemleri ile belirlenmiştir. Uçucu yağın, insan kolorektal adenokarsinom HT-29 hücre hattına karşı antiproliferatif etkisi Gerçek Zamanlı Hücre Analizörü xCELLigence yöntemi ile değerlendirilmiştir. Ayrıca, uçucu yağın toplam fenolik miktarı ve radikal giderim aktivitesi de tespit edilmiştir. Sonuçlar göstermektedir ki; timol, %40 timol : %60 karvakrol, %25 terpinen-4-ol: %75 karvakrol S. aureus ATCC 29213 suşlarına karşı önemli bir antimikrobiyal aktiviteye sahiptir. Bununla birlikte, uçucu yağ HT-29 hücre hattının büyümesi üzerine yüksek bir inhibisyon etki göstermiştir IC50

Anahtar Kelimeler

Satureja boissieri, antimikrobiyal, antiproliferatif, HT-29

Investigation of Antimicrobial, Antiproliferative and Antioxidant Properties of Satureja boissieri Essential Oil

Öz

The aim of this study was to investigate the biological activities of Satureja boissieri Hausskn. ex Boiss. Lami- aceae essential oil. Antimicrobial effects of the major components from the essential oil were determined by agar well and disc diffusion methods. Antiproliferative effect of the essential oil against human colorectal adenocarcinoma HT-29 cell line was evaluated by Real Time Cell Analyzer xCELLigence method. In addition, total phenolic content and radical scavenging activity of the essential oil were determined. The results show that thymol, thymol 40%:carvacrol 60%, and terpinene-4-ol 25%:carvacrol 75% had significant antimicrobial activity against S. aureus ATCC 25923 and S. aureus ATCC 29213 strains. Also, the essential oil exhibited the high inhibitory effect on the growth of HT-29 cell line IC50

Anahtar Kelimeler

Satureja boissieri, Antimicrobial, antiproliferative, HT-29

Kaynakça

• D. Adam, Global antibiotic resistance in Streptococcus pneumoniae, Journal of Antimicrobial Chemotherapy, 50 (2002) 1-5.
• B. Batabyal, G.K. Kundu, S. Biswas, Methicillin-Resistant Staphylococcus Aureus: A Brief Review, International Research Journal of Biological Sciences, 1 (2012) 65-71.
• J.-Y. Kim, K.-W. Park, K.-D. Moon, M.-K. Lee, J. Choi, S.-T. Yee, K.-H. Shim, K.-I. Seo, Induction of apoptosis in HT-29 colon cancer cells by crude saponin from Platycodi Radix, Food and Chemical Toxicology, 46 (2008) 3753-3758.
• A. Jemal, F. Bray, M.M. Center, J. Ferlay, E. Ward, D. Forman, Global cancer statistics, CA: a cancer journal for clinicians, 61 (2011) 69-90.
• H. Ohigashi, T. Osawa, J. Terao, S. Watanabe, T. Yoshikawa, Food factors for cancer prevention, Springer Science & Business Media, Tokya, Japan, 2013.
• N. Bisset, M. Witchtl, Herbal drugs and phytopharmaceuticals. A handbook for practice on a scientific basis. Boca Raton, Ann Arbor, London, Tokyo, CRC Press, (1994).
• F. Oke, B. Aslim, S. Ozturk, S. Altundag, Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten, Food Chemistry, 112 (2009) 874-879.
• F. Satil, T. Dirmenci, G. Tumen, Y. Turan, Commercial and Ethnic Uses of Satureja (Sivri Kekik) Species in Turkey, Ekoloji Dergisi, 17 (2008).
• F. Oke-Altuntas, I. Demirtas, A.R. Tufekci, S. Koldas, F. Gul, L. Behcet, H.I. Gecibesler, Inhibitory Effects of the Active Components Isolated from Satureja Boissieri Hausskn. Ex Boiss. On Human Cervical Cancer Cell Line, Journal of Food Biochemistry, 40 (2016) 499- 506.
• D. Kalemba, A. Kunicka, Antibacterial and antifungal properties of essential oils, Current Medicinal Chemistry, 10 (2003) 813-829.
• P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, R.H. Yolken, Manual of Clinical Microbiology, 8 ed., American Society for Microbiology, Washington, D.C., 2003.
• S. Koldas, I. Demirtas, T. Ozen, M.A. Demirci, L. Behcet, Phytochemical screening, anticancer and antioxidant activities of Origanum vulgare L. ssp. viride (Boiss.) Hayek, a plant of traditional usage, Journal of the science of food and agriculture, 95 (2015) 786-798.
• M.S. Blois, Antioxidant determinations by the use of a stable free radical, Nature, 181 (1958) 1199-1200.
• F. Oke, B. Aslim, Protective effect of two edible mushrooms against oxidative cell damage and their phenolic composition, Food Chemistry, 128 (2011) 613- 619.
• V. Singleton, J.A. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, American journal of Enology and Viticulture, 16 (1965) 144-158.
• H. Yegenoglu, B. Aslim, F. Oke, Comparison of Antioxidant Capacities of Ganoderma lucidum (Curtis) P. Karst and Funalia trogii (Berk.) Bondartsev & Singer by Using Different In Vitro Methods, Journal of Medicinal Food, 14 (2011) 512-516.
• M. Dal Pozzo, D. Santurio, L. Rossatto, A. Vargas, S. Alves, E. Loreto, J. Viegas, Activity of essential oils from spices against Staphylococcus spp. isolated from bovine mastitis, Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 63 (2011) 1229-1232.
• N. Gallucci, M. Oliva, E. Carezzano, J. Zygadlo, M. Demo, Terpenes antimicrobial activity against slime producing and non–producing Staphylococci, Mol. Med. Chem, 21 (2010) 132-136.
• J. Xu, F. Zhou, B.P. Ji, R.S. Pei, N. Xu, The antibacterial mechanism of carvacrol and thymol against Escherichia coli, Letters in Applied Microbiology, 47 (2008) 174-179.
• E. Souza, C. Oliveira, T. Stamford, M. Conceição, N. Gomes Neto, Influence of carvacrol and thymol on the physiological attributes, enterotoxin production and surface characteristics of Staphylococcus aureus strains isolated from foods, Brazilian Journal of Microbiology, 44 (2013) 29-35.
• S. Shah, P. Stapleton, P. Taylor, The polyphenol (−)‐ epicatechin gallate disrupts the secretion of virulence‐ related proteins by Staphylococcus aureus, Letters in applied microbiology, 46 (2008) 181-185.
• G. Klein, C. Rüben, M. Upmann, Antimicrobial Activity of Essential Oil Components Against Potential Food Spoilage Microorganisms, Current microbiology, 67 (2013) 1-9.
• C.F. Bagamboula, M. Uyttendaele, J. Debevere, 30. H. Miladi, R. Ben Slama, D. Mili, S. Zouari, A. Bakhrouf, Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri, Food Microbiology, 21 (2004) 33-42.
• G. Murgananthan, P. Sathyachethan, Antimicrobial constituents from Plants, International Research Journal of Pharmacy, 3 (2012) 5-9.
• J. Kurlenda, M. Grinholc, Alternative therapies in Staphylococcus aureus diseases, Acta Biochim Pol, 59 (2012) 171-184.
• Y. Le Loir, F. Baron, M. Gautier, Staphylococcus aureus and food poisoning, Genet Mol Res, 2 (2003) 63-76.
• S. Işık, A. Karagöz, Ş. Karaman, C. Nergiz, Proliferative and apoptotic effects of olive extracts on cell lines and healthy human cells, Food Chemistry, 134 (2012) 29- 36.
• I. Sadeghi, M. Yousefzadi, M. Behmanesh, M. Sharifi, A. Moradi, In vitro Cytotoxic and Antimicrobial Activity of Essential Oil From Satureja Intermedia, Iranian Red Crescent Medical Journal, 15 (2013) 70-74.
• I. Lampronti, A.M. Saab, R. Gambari, Antiproliferative activity of essential oils derived from plants belonging to the Magnoliophyta division, International journal of oncology, 29 (2006) 989-995. E. Ammar, Chemical composition and cytotoxic and antioxidant activities of Satureja montana L. essential oil and its antibacterial potential against Salmonella spp. strains, Journal of Chemistry, X 2013 (2013).
• A.T. Koparal, M. Zeytinoğlu, Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549, Cytotechnology, 43 (2003)149-154.
• M. Güllüce, M. Sökmen, D. Daferera, G. A‐ar, H. Özkan, N. Kartal, M. Polissiou, A. Sökmen, F. Şahi‐n, In Vitro Antibacterial, Antifungal, and Antioxidant Activities of the Essential Oil and Methanol Extracts of Herbal Parts and Callus Cultures of Satureja hortensis L, Journal of Agricultural and Food Chemistry, 51 (2003) 3958-3965.