Antiproliferative properties and evaluation of antioxidant of different cornelian cherry genotypes and analysis of phenolic and sugar compounds by HPLC

Yıl 2023, Cilt: 51 Sayı: 1, 57 - 70, 01.01.2023

Yılmaz Uğur , Emine Şalva , Fırat Karaat , Selim Erdoğan

https://doi.org/10.15671/hjbc.1065317

Cited By: 3

Öz

Bu çalışmanın amacı, kızılcık meyvelerinin kimyasal bileşimi (C vitamini, bireysel fenolik ve şeker bileşikleri) ve antioksidan kapasitesinin yanında, meyve ekstrelerinin sağlıklı (L-929) ve akciğer kanseri (A-549) hücreleri üzerindeki sitotoksik etkisini araştırmaktır. Türkiye'de aynı koşullarda yetiştirilen on üç kızılcık (Cornus mas L.) genotipi meyve örnekleri ASE tekniği ile optimum koşullarda ekstrakte edildikten sonra kimyasal bileşim, HPLC-DAD-RID ile analiz edildi. Antioksidan kapasitesi, toplam fenolik ve toplam antosiyanin içeriği spektrofotometrik yöntemler kullanılarak belirlendi. Sitotoksik etkiler, 48 saat boyunca L-929 ve A-549 hücre hatlarında MTT testi ile değerlendirildi. Meyve ekstraktlarının L-929 sağlıklı fare fibroblast hücreleri üzerinde toksik etkisi gözlenmezken, A-549 akciğer kanseri hücrelerinde hücre proliferasyonunu (yaklaşık %50) azalttığı belirlendi. A-549 hücreleri üzerindeki antioksidan kapasite ve sitotoksik etkiler için öne çıkan genotipler sırasıyla 44-03, 44-20, 44-21, 77-09 ve 44-21, 44-16, 77-05 olduğu belirlendi. Elde edilen sonuçlar, genotipler (p ≤ 0.05) arasında önemli farklılıklar olduğunu ve kızılcık meyvelerinin önemli bir antioksidan kapasiteye ve antiproliferatif etki potansiyeline sahip olduğunu ortaya koymuştur.

Anahtar Kelimeler

Kızılcık (Cornus mas L.), Fenolikler, C vitamin, Antioksidan kapasite, antiproliferative effect, akciğer kanseri, Kızılcık (Cornus mas L.), Fenolikler, C vitamin, Antioksidan kapasite, antiproliferative effect, akciğer kanseri

Proje Numarası

TDK-2018-1497

Kaynakça

• 1. G. Shui, LP. Leong (2006) Residue from star fruit as a valuable source for functional food ingredients and antioxidant nutraceuticals. Food Chem 97: 277–284. https://doi.org/10.1016/j.foodchem.2005.03.048
• 2. E. Büyüktuncel (2013) Toplam fenolik içerik ve antioksidan kapasite tayininde kullanilan baslica spektrofotometrik yöntemler. Marmara Pharm J 2: 93–103. https://doi.org/10.12991/201317377
• 3. A, Ekbul (2004) Diyetsel Polifenoller ve Kardiyovasküler Sistem. Turkiye Klin J Cardiol 17: 48–54.
• 4. K. Šavikin, G Zdunić, T Janković, T Stanojković, Z Juranić, N Menković (2009) In vitro cytotoxic and antioxidative activity of Cornus mas and Cotinus coggygria. Nat Prod Res 23: 1731–1739, doi:10.1080/14786410802267650. https://doi.org/10.1080/14786410802267650
• 5. BM. Popović, D. Štajner, K. Slavko, B. Sandra (2012) Antioxidant capacity of cornelian cherry (Cornus mas L.) comparison between permanganate reducing antioxidant capacity and other antioxidant methods. Food Chem 134: 734–741. https://doi.org/10.1016/j.foodchem.2012.02.170
• 6. S Celik, I Bakirci, IG Sat (2006) Physicochemical and organoleptic properties of yogurt with cornelian cherry paste. Int J Food Property 9: 401–408. https://doi.org/10.1080/10942910600596258
• 7. S Tural, I Koca (2008) Physico-chemical and antioxidant properties of cornelian cherry fruits (Cornus mas L.) grown in Turkey. Sci Hort 116: 362-366. https://doi.org/10.1016/j.scienta.2008.02.003
• 8. BM De, D Donno, MG Mellano, L Riondato, EN Rakotoniaina, GL Beccaro (2018) Cornus mas (L.) fruit as a potential source of natural health-promoting compounds: Physico-chemical characterization of bioactive components. Plant Foods Hum Nutr 73: 89–94. https://doi.org/10.1007/s11130-018-0663-4
• 9. S Cosmulescu I Trandafir, F Cornescu (2019) Antioxidant capacity, total phenols, total flavonoids, and a color component of cornelian cherry (Cornus mas L.) wild genotypes. Not Bot Horti Agrobo 47: 390-394. https://doi.org/10.15835/nbha47111375
• 10. GE Pantelidis, M Vasilakakis, GA Manganaris, G Diamantidis (2007) Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries, and cornelian cherries. Food Chem 102: 777-2783. https://doi.org/10.1016/j.foodchem.2006.06.021
• 11. B Moldovan, A Filip, S Clichici, R Suharoschi, P Bolfa, L David (2016) Antioxidant capacity of cornelian cherry (Cornus mas L.) fruits extract and the in vivo evaluation of ıts anti-inflammatory effects. J Functional Foods 26: 77- 87. https://doi.org/10.1016/j.jff.2016.07.004
• 12. H Hamid, H Yousef, H Jafar, A Mohammad (2011) Antioxidant capacity and phytochemical properties of cornelian cherry (Cornus mas L.) genotypes in Iran. Sci Hort 129: 459–463. https://doi.org/10.1016/j.scienta.2011.04.017
• 13. AS Milenkovic-Andjelkovic, MZ Andjelkovic, AN Radovanovic, BC Radovanovic, V Nikolic (2015) Phenol composition, DPPH radical scavenging and antimicrobial activity of cornelian cherry (Cornus mas) fruit and leaf extracts. Hem Ind 69: 331–337. https://doi.org/10.2298/HEMIND140216046M
• 14. S Erdoğan, S Erdemoğlu (2011) Evaluation of polyphenol contents in differently processed apricots using accelerated solvent extraction followed by high-performance liquid chromatography–diode array detector. Int J Food Sci Nutr 62: 729–739. https://doi.org/10.3109/09637486.2011.573469
• 15. JY Hwang, YS Shue, HM Chang (2001) Antioxidative activity of roasted and defatted peanut kernels. Food Res Int 34: 639–647. https://doi.org/10.1016/S0963-9969(01)00083-7
• 16. GC Yen, CY Hung (2000) Effects of alkaline and heat treatment on antioxidative activity and total phenolics of extracts from Hsian-Tsao (Mesona procumbens Hemsl.). Food Res Int 33: 487-492. https://doi.org/10.1016/S0963-9969(00)00073-9
• 17. NM Karaaslan (2012) Kiraz (Prunus avium), Çilek (Fragaria vesca) ve Kızılcık (Cornus Mas L.) Meyvelerindeki Antosiyanin Bileşiklerinin HPLC-ESI-MS İle Tayini ve Karakterizasyonu. PhD. Thesis, Faculty of Science, Fırat University, Elazığ, Turkey.
• 18. B Cemeroğlu (2007) Gıda Analizleri. Gıda Teknolojisi Yayınları. No:34 Ankara. s: 168-171.
• 19. FS Hosseini, M Noroozi Karimabad, MR Hajizadeh, A Khoshdel, SK Falahati-Pour, MR Mirzaei, SM Mirmohamadi, M Mahmoodi (2019) Evaluating of Induction of Apoptosis by Cornus mas L. Extract in the Gastric Carcinoma Cell Line (AGS). Asian Pacific J Cancer Prev 20: 123–130. https://doi.org/10.31557/APJCP.2019.20.1.123
• 20. Y Baran, C Oztekin, EY Bassoy (2010) Combination of Fludarabine and Imatinib Induces Apoptosis Synergistically Through Loss of Mitochondrial Membrane Potential and Increases in Caspase-3 Enzyme Activity in Human K562 Chronic Myeloid Leukemia Cells. Cancer Invest 28: 623–628. https://doi.org/10.3109/07357901003631056
• 21. Y Baran, AU Ural, U Gunduz (2007) Mechanisms of cellular resistance to imatinib in human chronic myeloid leukemia cells. Hematology 12: 497–503. https://doi.org/10.1080/10245330701384179
• 22. T Stiropoulos, A Petridis, N Koutinas, I Therios (2011) ‘Ntoulia 1’ and ‘Ntoulia 2’ cornelian cherries (Cornus mas L.). Hort Science 46: 955-957. https://doi.org/10.21273/HORTSCI.46.6.955
• 23. AZ Kucharska, AS Letowska (2011) Morphological, physical & chemical, and antioxidant profiles of Polish varieties of cornelian cherry fruit (Cornus mas L.). ŻYWNOŚĆ Nauka Technologia Jakość 3: 78 – 89. https://doi.org/10.15193/zntj/2011/76/078-089
• 24. J Cetkovska, P Divis, J Vespalcova, J Porizka, V Reznicek (2015) Basic nutritional properties of cornelian cherry (Cornus mas L.) cultivars grown in the Czech Republic. A Alim 44: 549-557. https://doi.org/10.1556/AAlim.2014.0013
• 25. B Yousefi, M Abasi, MM Abbasi, R Jahanban-Esfahlan (2015) Antiproliferative properties of Cornus mas fruit in different human cancer cells. Asian Pac J Cancer Prev 16: 5727-5731. https://doi.org/10.7314/apjcp.2015.16.14.5727
• 26. S Klymenko, AZ Kucharska, A Sokół-Łętowska, N Piórecki (2019) Antioxidant activities and phenolic compounds in fruits of cultivars of cornelian cherry (Cornus mas L.). Agr Bio Div Impr Nut Health Life Qual 484–499. https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.484-499
• 27. NK Kantar (2019) Kızılcık Meyvesinden (Cornus mas) Ohmik Destekli Mikrodalga ve Ultrasonik Yöntemleri İle Fenolik Bileşiklerin Ekstraksiyonu. PhD. Thesis, Faculty of Engineering, Ankara University, Ankara, Turkey.
• 28. BC Radovanovic, ASM Andelkovic, AB Radovanovic, MZ Andelkovic (2013) Antioxidant and antimicrobial activity of polyphenol extracts from wild berry fruits grown in Southeast Serbia. Tropical J Pharmaceutical Res 12: 813-839. https://doi.org/10.4314/tjpr.v12i5.23
• 29. G Tian, T Zhang, F Yang, Y Ito (2000) Separation of gallic acid from Cornus officinalis Sieb. Et Zucc by High-Speed Counter-Current Chromatography. J Chromatogr A 886: 309–312. https://doi.org/10.1016/S0021-9673(00)00480-5
• 30. W Du, H Cai, M Wang, X Ding, H Yang, B Cai (2008) Simultaneous determination of six active components in crude and processed Fructus corni by High-Performance Liquid Chromatography. J Pharm Biomed Anal 48: 194–197. https://doi.org/10.1016/j.jpba.2008.04.021
• 31. J Sochor, T Jurikova, S Ercisli, J Mlcek, M Baron, S Balla, SO Yılmaz, T Necas (2014) Charcterization of cornelian cherry (Cornus mas L.) genotypes–genetic resources for food production in the Czech Republic. Genetika 46: 915–924. https://doi.org/10.2298/GENSR1403915S
• 32. G Cao, Y Zhang, J Feng, H Cai, C Zhang, M Ding, X Cong, B Cai (2011) A rapid and sensitive assay for determining the main components in processed fructus corni by UPLC-Q-TOF-MS. Chromatographia 73: 135–141. https://doi.org/10.1007/s10337-010-1825-1
• 33. MH Lin, HK Liu, WJ Huang, CC Huang, TH Wu, FL Hsu (2011) Evaluation of the potential hypoglycemic and beta-cell protective constituents ısolated from corni fructus to tackle ınsulin-dependent diabetes mellitus. J Agric Food Chem 59: 7743–7751. https://doi.org/10.1021/jf201189r
• 34. S Cosmulescu, I Trandafir, V Nour (2017) Phenolic acids and flavonoids profiles of extracts from edible wild fruits and their antioxidant properties. Int J Food Prop 20: 3124–3134. https://doi.org/10.1080/10942912.2016.1274906
• 35. Z Liu, ZY Zhu, H Zhang, GG Tan, XF Chen, YF Chai (2011) Qualitative and quantitative analysis of Fructus corni using ultra sound assisted microwave extraction and high-performance liquid chromatography coupled with diode array UV detection and time-of-flight mass spectrometry. J Pharm Biomed Anal 55: 557–562. https://doi.org/10.1016/j.jpba.2011.02.007
• 36. H Cai, G Cao, B Cai (2013) Rapid simultaneous ıdentification and determination of the multiple compounds in crude fructus corni and its processed products by HPLC– MS/MS with multiple reaction monitoring mode. Pharm Biol 51: 273–278. https://doi.org/10.3109/13880209.2012.720689
• 37. S Deng, BJ West, CJ Jensen (2013) UPLC-TOF-MS characterization and ıdentification of bioactive iridoids in Cornus mas fruit. Journal of Analytical Methods in Chemistry. Article ID 710972, 7 pages.
• 38. A Begic-Akagic, P Drkenda, A Vranac, P Orazem, M Hudina (2013) Influence of growing region and storage time on phenolic profile of cornelian cherry jam and fruit. Eur J Hort Sci 78: 30–39.
• 39. P Drkenda, A Spahic, A Begic-Akagic, F Gasi, A Vranac, M Hudina, M Blanke (2014) Pomological characteristics of some autochthonous genotypes of cornelian cherry (Cornus mas L.) in Bosnia and Herzegovina. Erwerbs-Obstbau 56: 59–66. https://doi.org/10.1007/s10341-014-0203-9
• 40. JM Harnly, RF Doherty, GR A Beecher, JM Holden (2006) Flavonoid content of U.S. fruits, vegetables, and nuts. J Agric Food Chem 54: 9966−9977. https://doi.org/10.1021/jf061478a
• 41. S Erdoğan (2008) Çeşitli Kayısı Örneklerinde Bakır Spesiasyonu (Türlendirme). PhD. Thesis, Faculty of Science, İnönü University, Malatya, Turkey.
• 42. IB Perova, AA Zhogova, AV Poliakova, KI Éller, GV Ramenskaia, IA Samylina (2014) Biologically active substances of cornelian cherry fruits (Cornus mas L.). Vopr Pitan 83: 86‐94.
• 43. NT Petkova, MH Ognyanov (2018) Phytochemical characteristics and in vitro antioxidant activity of fresh, dried, and processed fruits of cornelian cherries (Cornus mas L.). Bulgarian Chem Communications 50: 302 –307.
• 44. T Tarko, A Duda-Chodak, P Satora, P Sroka, P Pogoń, J Machalica (2014) Chaenomeles japonica, Cornus mas, Morus nigra fruits characteristics and their processing potential. J Food Sci Technol 51: 3934–3941. https://doi.org/10.1007/s13197-013-0963-5
• 45. H Antolak, A Czyzowska, M Sakac, A Misan, O Duragic, D Kregiel (2017) Phenolic compounds contained in little-known wild fruits as antiadhesive agents against the beverage-spoiling bacteria Asia spp, Molecules 22: 1256. https://doi.org/10.3390/molecules22081256
• 46. A Kourpeti-Tiptiri, E Fitsiou, K Spyridopoulou (2019) Evaluation of antioxidant and antiproliferative properties of Cornus mas L. fruit juice. Antioxidants 8: 377. https://doi.org/10.3390/antiox8090377
• 47. FS Hosseini, MN Karimabad, MR Hajizadeh, A Khoshdel (2018) Evaluating of induction of apoptosis by Cornus mas L. extract in the Gastric Carcinoma Cell Line (AGS). Asian Pac J Cancer Prev 20: 123-130. https://doi.org/10.31557/APJCP.2019.20.1.123
• 48. Y Lu, BN Pekerti, ZS Toh, F Broom, G Savage, SQ Liu, D Huang (2017) Physicochemical parameters and proanthocyanidin profiles of cranberries cultivated in New Zealand. J Food Compos Anal 63: 1-7. https://doi.org/10.1016/j.jfca.2017.07.002
• 49. PN Brown, SJ Murch, P Shipley (2012) Phytochemical diversity of cranberry (Vaccinium macrocarpon Aiton) cultivars by anthocyanin determination and metabolomic profiling with chemometric analysis. J Agric Food Chem 60: 261-271. https://doi.org/10.1021/jf2033335