Molecular Typing with PFGE Method of Methicillin-resistant Staphylococcus aureus Isolates from in a University Hospital

Yıl 2021, , 11 - 14, 16.12.2021

Sümeyra Savaş , Mehmet Parlak

https://doi.org/10.29228/HMJ.3

Öz

Objectives: Staphylococcus aureus is a pathogen that causes many infections in humans, and the emergence of methicillin-resistant (MRSA) isolates in the last fifty years poses a threat to society and hospitals. Preventing the spread of MRSA strains between and within hospitals can only be achieved with a correct typing. The PFGE method is considered to be the gold standard among molecular typing methods.

Methods: In this study, a clonal relationship between nine methicillin-resistant S. aureus isolates isolated from Van Yüzüncü Yıl University hospital was determined .Pfge analysis was done with the Bionumerics program. The similarity index was created at a tolerance value of 1% using the “Dice” coefficient. The clustering relationship between isolates was demonstrated using the UPGMA (unweighted pair group method of arithmetic averages) method. The principles set out by Tenover et al. were adopted for the evaluation of genotypic association between isolates

Results: As a result of the analysis 4 independent genotypes were identified. It was determined that there were 2 related isolates in 3 genotypes.

Conclusion: In our study, it was determined that there was not a dominant MRSA clone in different years in the hospital studied, since samples from different years were studied. However, it is thought that it would be beneficial to increase the precautions in order to prevent the distribution of infection in all hospitals and to control the infection in all areas.

Anahtar Kelimeler

Methicillin-resistant Staphyococcus aureus, Molecular typing, Pulsed Field Gel Electrophoresis, molecular epidemiology

Bir Üniversite Hastanesinden Izole Edilen Metisilin Dirençli Stapylococcus Aureus Izolatlarının PFGE Yöntemi ile Moleküler Tiplendirilmesi

Öz

Amaç
Staphylococcus aureus insanlarda birçok enfeksiyona neden olan bir patojen olup, son elli yılda metisiline dirençli (MRSA) izolatların ortaya çıkması toplum ve hastaneler için tehdit oluşturmaktadır. MRSA suşlarının hastaneler arasında ve hastaneler içinde yayılmasının önlenmesi ancak doğru bir tipleme ile sağlanabilir. PFGE yöntemi, moleküler tipleme yöntemleri arasında altın standart olarak kabul edilmektedir.
Gereç ve Yöntem
Bu çalışmada, Van Yüzüncü Yıl Üniversitesi hastanesinden izole edilen dokuz metisiline dirençli S. aureus izolatı arasında klonal bir ilişki belirlendi. Bionumerics programı ile PFGE analizi yapıldı. Benzerlik indeksi “Dice” katsayısı kullanılarak %1 tolerans değerinde oluşturuldu. İzolatlar arasındaki kümeleme ilişkisi, UPGMA (ağırlıksız çift grup aritmatik ortalamalar yöntemi) yöntemi kullanılarak gösterildi. Tenover ve arkadaşlarının kriterleri izolatlar arasındaki genotipik ilişkinin değerlendirilmesi için kabul edildi.
Bulgular
Analiz sonucunda 4 bağımsız genotip tespit edilmiştir. 3 genotipte 2 ilişkili izolat olduğu belirlendi.SonuçÇalışmamızda, farklı yıllardan örnekler çalışıldığı için çalışılan hastanede farklı yıllarda baskın bir MRSA klonu olmadığı belirlendi. Ancak enfeksiyonun tüm hastanelerde yayılmasını önlemek ve enfeksiyonun her alanda kontrol altına alınması için tedbirlerin artırılmasının faydalı olacağı düşünülmektedir.

Anahtar Kelimeler

Metisilin Dirençli Staphyococcus aureus, Moleküler Tiplendirme, Değişken Alanlı Jel Elektroforezi, Moleküler Epidemiyoloji

Kaynakça

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