İlk Kez Epilepsi Tanısı Konulmuş Çocuklarda Apelin Düzeyinin ve Oksidatif Hasarın Araştırılması

Öz

Amaç: Dünya genelinde 65 milyon kişiyi olumsuz etkileyen epilepsi hastalığı, cinsiyet ayrımı olmaksızın tüm yaş gruplarında görülebilmektedir. Mekanizması henüz bilinmemekle birlikte, hastalık insan hastalıklarının tarihi boyunca bilinmektedir. Bu çalışmada epilepsili çocuklarda apelin ve oksidatif stres düzeylerinin araştırılması amaçlanmıştır. Materyal ve Metod: Çalışmaya epilepsi tanısı konan ve tedaviye başlamamış epilepsili (0-16 yaş) çocuklar dahil edildi. Çalışma, kontrol grubu sağlıklı normal çocuklar (n=28) ve epilepsili çocuklar (n=28) olmak üzere toplam 56 çocukta yapıldı. Apelin, ileri protein oksidasyon ürünü (AOPP) ve DNA hasar markeri 8-Hidroksi 2-Deoksiguanozin (8-OHdG) seviyeleri analizleri için ELISA yöntemi ile kan örnekleri alındı. Bulgular: Jeneralize tip epilepside apelin düzeyi kontrol grubuna ve komplike ateşli gruba göre daha düşüktü (p<0.05). Epilepsi nöbet sayısının jeneralize tip epilepside daha fazla olduğu bulundu (p<0.05). Apelin artışına bağlı olarak nöbet sayısı azalırken (p=0.05; r=-0.260), AOPP artışına bağlı olarak arttı (p=0.05; r=0.264). AOPP, fokal tip epilepside kontrol grubuna göre daha yüksekti. Sonuç: Sonuç olarak; 1) Apelin, oksidatif DNA hasarını önleyerek nöbet sayısını azalttı, 2) AOPP artışı ile nöbet sayısını artırdı, 3) Yaş arttıkça AOPP düzeyindeki azalma nedeniyle nöbet sayısının azaldığı belirlendi.

Anahtar Kelimeler

• Epilepsi
• Apelin
• Oksidatif stres
• 8-OHdG
• AOPP

Proje Numarası

Project # 17051

Investigation of Apelin Level and Oxidative Damage in Children Diagnosed with Epilepsy for the First Time

Öz

Background: Epilepsy disease, which negatively affects 65 million people around the world, can be seen in all age groups regardless of gender. Disease is known throughout the history of human disease, although the mechanism is still unknown. In this study, it is aimed to investigate of apelin and oxidative stress levels in children with epilepsy. Materials and methods: Children with epilepsy (aged 0-16) who had been diagnosed with epilepsy and did not start treatment were included in the study. This study included control group healthy normal children (n=28) and children with epilepsy group (n=28), totally 56 children were included. Blood samples were removed for apelin, advanced protein oxidation product (AOPP) and DNA damage marker 8-Hydroxy 2-Deoxyguanosine (8-OHdG) levels analyses by ELISA method. Results: Apelin level in generalized type epilepsy was lower than the control group and the complicated febrile group (p<0.05). It has been found that the number of epilepsy seizures is more common in the generalized type epilepsy (p<0.05). While the number of seizures decreased due to the increase in apelin (p=0.05; r=-0.260), it increased due to the increase in AOPP (p=0.05; r=0.264). AOPP was higher in focal type epilepsy than control group. Conclusions: Consequently; 1) Apelin reduced the number of seizures by preventing oxidative DNA damage, 2) Increased the number of seizures by the AOPP increase, 3) As the age rises, the number of seizures has been determined to lower due to decreased in AOPP level.

Anahtar Kelimeler

• Epilepsy
• Apelin
• Oxidative stress
• 8-OHdG
• AOPP

Destekleyen Kurum

HÜBAP

Proje Numarası

Project # 17051

Teşekkür

None

Kaynakça

1. 1. Avoli M, Louvel J, Pumain R, Kohling R. Cellular and molecular mechanisms of epilepsy in the human brain. Progress in Neurobiology. 2005;77(3):166-200.
2. 2. Wendling F, Bartolomei F, Bellanger JJ, Chauvel P. Epileptic fast activity can be explained by a model of impaired GABAergic dendritic inhibition. Eur J Neurosci. 2002;15(9):1499-508.
3. 3. Reaux A, Gallatz K, Palkovits M, Llorens-Cortes C. Distribution of apelin-synthesizing neurons in the adult rat brain. Neuroscience. 2002;113(3):653-62.
4. 4. Zhang XG, Peng X, Fang M, Zhou CL, Zhao FH, Zhang Y, et al. Up-regulation of apelin in brain tissue of patients with epilepsy and an epileptic rat model. Peptides. 2011;32(9):1793-9.
5. 5. England MJ, Liverman CT, Schultz AM, Strawbridge LM. Epilepsy across the spectrum: promoting health and understanding. A summary of the Institute of Medicine report. Epilepsy Behav. 2012;25(2):266-76.
6. 6. Minardi C, Minacapelli R, Valastro P, Vasile F, Pitino S, Pavone P, et al. Epilepsy in Children: From Diagnosis to Treatment with Focus on Emergency. J Clin Med. 2019;8(1).
7. 7. Loscher W. Animal models of intractable epilepsy. Prog Neurobiol. 1997;53(2):239-58.
8. 8. Avanzini G, Franceschetti S. Cellular biology of epileptogenesis. Lancet Neurol. 2003;2(1):33-42.

9. 9. Manea MM, Sirbu A, Dragos D, Dobri AM, Sirbu AG, Tuta S. Epileptic Seizures as the First Manifestation of Fahr's Syndrome. Acta Endocrinol (Buchar). 2020;16(3):370-4.
10. 10. Kalantaripour TP, Esmaeili-Mahani S, Sheibani V, Asadi-Shekaari M, Pasban-Aliabadi H. Anticonvulsant and neuroprotective effects of apelin-13 on pentylenetetrazole-induced seizures in male rats. Biomed Pharmacother. 2016;84:258-63.
11. 11. Elhady M, Youness ER, Mostafa RSI, Aziz AA, Hussein R. Oxidative stress contribution to attention deficit hyperactivity disorder in children with epilepsy. Appl Neuropsych-Chil. 2019;8(4):347-54.
12. 12. Meral C, Cekmez F, Vurucu S, Tascilar E, Pirgon O, Canpolat FE, et al. New adipocytokines (vaspin, apelin, visfatin, adiponectin) levels in children treated with valproic acid. Eur Cytokine Netw. 2011;22(2):118-22.
13. 13. Dizdaroglu M. Facts about the artifacts in the measurement of oxidative DNA base damage by gas chromatography mass spectrometry. Free Radical Res. 1998;29(6):551-63.
14. 14. Jarrett SG, Liang LP, Hellier JL, Staley KJ, Patel M. Mitochondrial DNA damage and impaired base excision repair during epileptogenesis. Neurobiol Dis. 2008;30(1):130-8.
15. 15. Haznedar P, Dogan O, Albayrak P, Tuncer GO, Teber S, Deda G, et al. Effects of levetiracetam and valproic acid treatment on liver function tests, plasma free carnitine and lipid peroxidation in childhood epilepsies. Epilepsy Res. 2019;153:7-13.
16. 16. Migliore L, Fontana I, Colognato R, Coppede F, Siciliano G, Murri L. Searching for the role and the most suitable biomarkers of oxidative stress in Alzheimer's disease and in other neurodegenerative diseases. Neurobiol Aging. 2005;26(5):587-95.
17. 17. Perry G, Cash AD, Smith MA. Alzheimer Disease and Oxidative Stress. J Biomed Biotechnol. 2002;2(3):120-3.
18. 18. Grosso S, Longini M, Rodriguez A, Proietti F, Piccini B, Balestri P, et al. Oxidative stress in children affected by epileptic encephalopathies. J Neurol Sci. 2011;300(1-2):103-6.
19. 19. Pedre LL, Gallardo JM, Chacon LMM, Garcia AV, Flores-Mendoza M, Neri-Gomez T, et al. Oxidative Stress in Patients with Drug Resistant Partial Complex Seizure. Behav Sci. 2018;8(6).