Evaluation the Effect of 50 g Glucose Tolerance Test on Oxidative Stress and Interleukin-8 Parameters in Prediabetic Pregnancy

Öz

Background: The aim of this study is to evaluate the effect of 50 g oral glucose tolerance test (OGTT) on oxidative stress and interleukin-8 (IL-8) parameters in prediabetic pregnant women. Materials and Methods: Fasting and 1 hour blood samples were collected from 79 pregnant women who were administered 50 gr OGTT. Patients with a 1 hour blood glucose level of 140–200 mg/dl were considered as the prediabetic group. Thereafter, routine biochemical parameters and the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) and interleukin-8 (IL-8) parameters were measured from the serum samples taken during fasting and at 1 hour. Results: The serum GPx and SOD levels of the prediabetic group were remarkably lower than that of the control group (p<0.05). Whereas the serum IL-8 levels of the prediabetic group were significantly higher than that of the control group (p<0.05). When the fasting and 1 hour levels of the parameters were compared during OGTT, SOD levels were significantly decreased (p<0.001) and IL-8 levels were significantly higher (p<0.001). Conclusions: In conclusion, it was found that serum GPX and SOD levels decreased, while serum IL-8 levels increased in prediabetic pregnant women; however, when 50 g OGTT was administered to these patients, SOD levels decreased and IL-8 levels increased. These results we obtained suggest that oxidative stress and systemic inflammation that are already present in prediabetic pregnant women may be triggered by 50 g OGTT, posing negative risk factors for pregnant women.

Anahtar Kelimeler

• inflammation
• OGTT
• oxidative stress
• prediabetes

Prediyabetik Gebelerde 50 gr Glukoz Yükleme Testinin Oksidatif Stres ve İnterlökin-8 Parametreleri Üzerine Etkisinin Değerlendirilmesi

Öz

Amaç: Gestasyonel diyabetes mellitus (GDM), gebelikte β-hücre fonksiyon bozukluğu ve insülin direncinin metabolik defektleri ile karakterize, gebeliğin yaygın bir tıbbi komplikasyonudur. Bu çalışmada, prediabetik gebelerde 50 gr oral glukoz tolerans testi (OGTT)’nin oksidatif stres ve interlökin-8 (IL-8) parametreleri üzerine etkisinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: 50 gr OGTT yapılan 79 gebeden açlık ve 1. saat kan numuneleri alındı. Birinci saat kan şekeri 140– 200 mg/dl olan hastalar prediyabetik grup olarak kabul edildi. Daha sonra açlık ve 1. saatte alınan serum numunelerinde, rutin biyokimya parametrelerinin ve süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), malondialdehit (MDA) ve interlökin-8 (IL-8) parametrelerinin düzeyleri ölçüldü. Bulgular: Prediabetik grup serum GPx ve SOD düzeyleri, kontrol grubundan anlamlı derecede düşük bulundu (p<0,05). Prediabetik grup serum IL-8 düzeyleri ise kontrol grubundan anlamlı olarak yüksek bulundu (p<0,05). OGTT sırasında parametrelerin açlık ve 1. saat düzeyleri karşılaştırıldığında ise SOD düzeylerinde anlamlı derecede azalma (p<0,001) ve IL-8 düzeylerinde ise anlamlı olarak yükseklik bulundu (p<0,001). Sonuç: Sonuç olarak prediabetik gebelerde serum GPX ve SOD düzeylerinde azalma, serum IL-8 düzeylerinde ise artış olduğu, bununla birlikte bu hastalara 50 gr OGTT yapılması durumunda da SOD düzeylerinde azalma ve IL-8 düzeylerinde ise artış olduğu bulundu. Bulduğumuz bu sonuçlar bize prediabetik gebelerde zaten var olan oksidatif stres ve sistemik inflamasyonun 50 gr OGTT ile daha da tetiklenerek gebe için olumsuz risk faktörleri oluşturabileceğini düşündürdü.

Anahtar Kelimeler

• inflamasyon
• OGTT
• oksidatif stres
• prediyabet

Kaynakça

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