Comparison of Hepatoprotective Effects of Metformin, Vitamin E and Black Seed Oil on Liver Tissue in Experimental Diabetic Rats

Öz

Background: The aim of the study was to compare the hepatoprotective effects of metformin, vitamin E and black seed oil against liver damage developed in diabetic rats.
Materials and Methods: 30 Wistar Albino rats Control (n:6), Diabetes (n:6), Diabetes + Black Cumin Oil (n:6), Diabetes + Vitamin E (n:6) and Diabetes + Metformin (n:6) were divided into 5 groups. In order to induce diabetes, a single dose of 45 mg/kg Streptozotocin was administered intraperitoneally. 100 mg/kg/day vitamin E was administered to the Diabetes + Vitamin E group, 100 mg/kg/day metformin to the Diabetes + Metformin group, and 2.5 ml/kg/day black seed oil to the Diabetes + Black Cumin oil group. Paraffin sections obtained from liver tissues were stained with Hematoxylin-Eosin, Masson Trichrome and PAS stains.
Results: Vacuolization, pycnotic core, dilatation of sinusoids, decreased glycogen content and congestion were observed in liver sections of diabetes group. There was partial improvement in the damage findings in the metformin group and minimal improvement in the vitamin E group. It was seen that the black seed oil group had a better effect in the evaluation of damage scoring than other treatment applications. p<0.01.
Conclusions: It is thought that black cumin oil may have healing effects against liver damage caused by diabetes.

Deneysel Diyabetik Sıçanlarda Metformin, E Vitamini ve Çörek Otu Yağının Karaciğer Dokusu Üzerine Hepatoprotektif Etkilerinin Karşılaştırılması

Öz

ÖZET Amaç: Amacımız, diyabetik ratlarda gelişen karaciğer hasarına karşı Metformin, E vitamini ve Çörek otu yağının hepotoprotektif etkilerini karşılaştırmaktır. Materyal ve Metod: 30 adet Wistar Albino rat, Kontrol (n:6), Diyabet (n:6), Diyabet + Çörek Otu Yağı (n:6), Diyabet + E Vitamini (n:6) ve Diyabet + Metformin (n:6) olarak 5 gruba ayrıldı. Diyabeti indüklemek amacıyla tek doz 45 mg/kg Streptozotosin (STZ) intraperitoneal yolla uygulandı. Diyabet + E vitamini grubuna 100 mg/kg/gün E vitamini, Diyabet + Metformin grubuna 100 mg/kg/gün metformin ve Diyabet + Çörek otu yağı grubuna 2.5 ml/kg/gün çörek otu yağı oral yollarla uygulandı. Karaciğer dokularından elde edilen parafin kesitler Hematoksilen-Eozin, Masson Trikrom ve PAS boyaları ile boyandı. Bulgular: Diyabet grubu karaciğer kesitlerinde vakuolizasyon, piknotik çekirdek, sinüzoidlerde dilatasyon, azalan glikojen içeriği ve konjesyon izlendi. Metformin grubunda hasar bulgularında kısmen ve E vitamini grubunda ise minimal iyileşme görüldü. Çörek otu yağı grubunun bulguları ise kontrol grubuna benzerdi. Sonuç: Diyabette gelişen karaciğer hasarına karşı çörek otu yağının etkili olabileceği belirlendi.

Anahtar Kelimeler

• Çörek otu yağı
• Diyabet
• E vitamini
• Karaciğer
• Metformin

Kaynakça

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