Investigation Plasma Amino Acids Profile in Patients With Bronchiectasis

Öz

Background: Bronchiectasis is a chronic respiratory disease characterized by a chronic cough, sputum production and irreversible pathological expansion of the bronchi associated with recurrent respiratory infections. Amino acids play an important role in catabolic and anabolic processes in health and disease. Data on the importance of amino acids in how and where metabolished in certain infections and diseases were mentioned in the available literature. Therefore, we evaluated plasma levels of some different amino acids profiles in patients with bronchiectasis. Materials and Methods: Blood samples were taken from 50 bronchiectasis patients and 30 healthy indivi-duals people who had no current disease history or any pathological conditions. Plasma amino acid profiles were measured in LC-MS/MS device. Results: In bronchiectasis patients, the plasma levels of alanine, arginine, citrulline, glutamine, glycine, lysine, ornithine, phenylalanine, proline, hydroxy proline, valine, anserine, and 1-methyl histidine were significantly increased whereas plasma levels of serine, cysteine, hitamine, glutamic acid and alpha amino adipic acid were significantly decreased when compared to the control group (P<0.01). Statistically no significant change in the levels of other amino acids such as histidin, and methyonin was observed. Conclusions: We think that examining the amino acid profile, especially in bronchiectasis, in order to clarify the role of metabolism in the application of new treatment in the inflammatory process, may contribute to shortening the diagnosis and treatment times.

Bronşektazi hastalarda Serum Amino Asit Profilinin İncelenmesi

Öz

Amaç Bronşektazi, kronik öksürük, balgam üretimi ve tekrarlayan solunum yolu enfeksiyonları ile ilişkili bronşların geri dönüşümsüz patolojik genişlemesi ile karakterize kronik bir solunum yolu hastalığıdır. Amino asitler, sağlık ve hastalıkta katabolik ve anabolik süreçlerde önemli bir rol oynar. Amino asitlerin bazı enfeksiyon ve hastalıklarda nasıl ve nerede metabolize edildiğine ilişkin veriler mevcut literatürde belirtilmiştir. Bu nedenle bronşektazili hastalarda bazı farklı amino asit profillerinin plazma düzeylerini değerlendirdik. Gereç ve Yöntem: Bronşektazili 50 hastadan ve mevcut hastalık öyküsü veya herhangi bir patolojik durumu olmayan 30 sağlıklı bireyden kan örnekleri alındı. Plazma amino asit profilleri LC-MS/MS cihazında ölçüldü. Bulgular: Bronşektazili hastalarda plasma amino asit profilineki amino asitlerin alanin, arjinin, sitrulin, glutamin, glysine, lizin, ornitin, fenilalanin, proline, hidroksi prolin, valin, anserin, 1-metil histidinin kontrol grubu ile karşılaştırıldığında anlamlı olarak arttığını (P<0.01), ancak serin, sistein, hitamin, taurin, glutamik asit ve alfa amino adipik asitin kontrol grubu ile karşılaştırıldığında anlamlı olarak düştüğü saptandı (P<0.01). Diğer amino asitler histidin, ve metyonin istatiksel olarak anlamlı bir değişiklik olmadı. Sonuç Amino asitler proteinlerin başlıca yapı taşlarını oluşturur. Kronik inflamatuarda sitrulin, arginin, triptofan, gylisin, lizin fenil alanin metabolizmaları da rol alır. Bu amino asitlerin endotelde önemli yer tutmaktadır, plasma amino asitlerinin seviyeleri vücuttaki amino asitlerin akışına tesir eden tüm faktörlerin net tesirini göstermektedir. İnflamatuar mekanizmlarda metabolizmanın yeni tedavi uygulama rolünü açıklığa kavuşturmak için özellikle broşktazi da amino asit profilinin incelenmesi, tanı ve tedavi sürelerini kısaltmada katkı sağlayabileceğıni ve daha geniş çaplı araştırmalar yapılması görüşündeyiz

Anahtar Kelimeler

• Bronşektazi hastalar
• Serbest Aminoasit profili
• LC-MS/MS

Kaynakça

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