Evaluation of Total Oxidant and Antioxidant Capacities in the Patients with Osteomyelitis

Öz

Background: Osteomyelitis is a progressive disease that occurs due to damage to bone tissue caused by a microorganism through infectious and inflammatory processes. It is believed that free oxygen radicals may play a role in the pathogenesis of osteomyelitis. This study aimed to evaluate total oxidant and total antiox-idant levels in blood samples taken from 30 patients diagnosed with osteomyelitis. Materials and Methods: Between January 2008 and September 2010, 30 patients diagnosed with osteomy-elitis, either as outpatient sorin patients, were included from the Departments of Infectious Disease and Clinical Microbiology and Orthopedics and Traumatology, along with 30 healthy volunteers without osteo-myelitis. The Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) levels of both the patients and the control group were measured using a fully automated colorimetric method. The Oxidative Stress Index (OSI) was calculated by determining the TOS/TAS ratio. The TAS, TOS, and OSI levels of the patients were compared with the results of healthy individuals. Results: Among the 30 osteomyelitis cases included in the study, 20 (67.7%) were male. There were no statistically significant differences between the patient and control groups in terms of age, gender, and body mass index (p>0.05). TOS and OSI levels were higher in osteomyelitis patients and lower in the con-trol group, with a significant difference between the groups (p=0.001). Conversely, TAS levels were lower in the osteomyelitis group and higher in the control group (p=0.024). Conclusions: The results of the study implicated that the patients with osteomyelitis were exposed to a severe oxidative stres and the estimation of TAS, TOS and OSI levels may be used as biochemical parameters to follow up these patients, and also that a supportive treatment with antioxidants may improve osteomye-litis therapy. However, more comprehensive studies with larger populations should be conducted to con-firm this result. Keywords: Osteomyelitis, TAS, TOS, OSI

Osteomiyelitli Hastalarda Oksidan ve Antioksidan Kapasitelerin Değerlendirilmesi

Öz

Amaç: Osteomiyelit; bir mikroorganizmanın infeksiyöz ve inflamatuar süreçler ile kemik dokuda hasar yaratması sonucu ortaya çıkan ilerleyici karakterde bir hastalıktır. Osteomiyelitin patogenezinde serbest oksijen radikallerinin rolü olabileceği düşünülmektedir. Bu çalışmada osteomiyelit tanısı alan 30 hastadan alınan kan örneklerinde total oksidan ve total antioksidan seviyelerin değerlendirilmesi amaçlandı. Materyal ve Metod: Ocak 2008 ile Eylül 2010 tarihleri arasında Enfeksiyon Hastalıkları ve Klinik Mikrobiy-oloji ile Ortopedi ve Travmatoloji Kliniklerinde ayaktan ve/veya yatarak osteomiyelit tanısı alan 30 hasta ve osteomiyeliti olmayan 30 sağlıklı gönüllü çalışmaya alındı. Hastaların ve kontrol grubunun TAS (Total anti-oksidan seviye) ve TOS (Total oksidan seviye) düzeyleri tam otomatik kolorimetrik yöntemle çalışıldı. TOS/TAS oranı hesaplanarak oksidatif stres indeksi (OSİ) bulundu. Hastaların TAS, TOS ve OSİ düzeyleri sağlıklı kişilerin sonuçları ile kıyaslandı. Bulgular: Çalışmaya alınan 30 osteomiyelit olgusunun 20’si (%67,7) erkek idi. Hasta ve kontrol grupları arasında yaş, cinsiyet ve beden kitle indeksi ortalaması açısından istatistiksel olarak anlamlı fark yoktu (p>0,05). TOS ve OSİ düzeyleri osteomiyelitli hastalarda yüksek, kontrol grubunda ise düşüktü ve gruplar arasında anlamlı fark vardı (p=0,001). TAS düzeyi ise osteomiyelitli hasta grubunda düşük, kontrol grubunda yüksekti (p=0,024). Sonuç: Çalışma sonuçları osteomiyelitli hastaların şiddetli oksidatif strese maruz kaldıklarını, TAS, TOS ve OSİ seviye ölçümlerinin bu hastaların takibinde kullanılabilecek biyokimyasal parametreler olduğunu ve ayrıca tedaviye destekleyici olarak antioksidanların eklenmesi ile gelişme sağlanabileceğini düşündürmektedir. Ancak bu sonucu teyit edebilmek için daha geniş popülasyonlarla daha kapsamlı yapılacak çalışmalara gerek vardır.

Anahtar Kelimeler

• Osteomiyelit
• TAS
• TOS
• OSİ

Etik Beyan

Bu çalışma tezden üretilmiştir. Çalışma için Şanlıurfa Klinik Araştırmalar Etik Kurul Başkanlığı’ndan onay alınmıştır (09.12.2009 tarih ve 2009.12.03 sayılı karar).

Kaynakça

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