Keçiboynuzu ekstrelerinin insan servikal kanser hücreleri üzerindeki antiproliferatif ve sitotoksik etkileri

Öz

Amaç: Leguminosae familyasının bir üyesi olan Ceratonia siliqua L., (keçiboynuzu, harnup)’nın antimikrobiyal, antifungal ve sitotoksik aktiviteye sahip olduğu bilinmektedir. Bu çalışmada, keçiboynuzunun yaprak ve meyvelerinin etli kısımlarından metanol ve etanol ile hazırlanan ekstrelerin, HeLa hücreleri üzerindeki antiproliferatif ve sitotoksik etkileri araştırılmıştır. Ayrıca embriyonik fare fibroblast hücre soyu olan 3T3 hücreleri kontrol olarak kullanılmıştır.

Materyal ve Metod: Bu amaçlarla 48 saat süreyle ve farklı konsantrasyonlarda (200-800μg/mL) ekstre uygulamasının ardından canlı hücre sayıları (proliferasyon, MTT yöntemi), mitotik indeks (mitotik aktivite) ve işaretlenme indeksleri (DNA sentezi) tespit edilmiştir. Ayrıca ekstrelerde bulunan fenolik bileşen kompozisyonları da LC-MS/MS ile belirlenmiştir.

Bulgular: Elde edilen bulgulara göre keçiboynuzunun metanolik ve etanolik yaprak ekstreleri insan servikal kanser HeLa hücrelerinin DNA sentezini inhibe ederek mitotik aktivitesini ve proliferasyonunu baskılamıştır. Ancak bu ekstreler aynı zamanda normal fibroblast 3T3 hücrelerine karşı da sitotoksik etki göstermişlerdir. Etanolik meyve ekstresi HeLa hücrelerinin DNA sentezini azaltmıştır.

Sonuç: C. siliqua bitkisinden elde edilecek etken maddeler veya hazırlanacak preparasyonlar antikanser ajan olabilme potansiyeli taşıyabilir. Ancak normal hücreler üzerindeki sitotoksik etkiler de göz ardı edilmemelidir.

Anahtar Kelimeler

• HeLa hücreleri,3T3 hücreleri,Keçiboynuzu,Sitotoksisite,3T3 hücreleri

Antiproliferative and cytotoxic effects of carob extracts on human cervical cancer cells

Öz

Background: Ceratonia siliqua L., a member of the family Leguminosae, has been reported to have antimicrobial, antifungal and cytotoxic activity (carob, kharnub). In this study, the antiproliferative and cytotoxic effects of extracts prepared with methanol and ethanol from the carob leaves and fruits on HeLa cells were investigated. Additionally, 3T3, embryonic mouse fibroblast cell line, was used as a control.

Material and Methods: For these purposes, after treatment of the extracts at different concentrations (200-800μg/mL) for 48 hours, viable cell counts (proliferation, MTT method), mitotic index (mitotic activity) and labeling index (DNA synthesis) were determined. In addition, the phenolic component compositions in the extracts were also determined by LC-MS / MS.

Results: According to the findings, the methanolic and ethanolic leaf extracts of carob bean suppressed mitotic activity and proliferation by inhibiting the DNA synthesis of human cervical cancer HeLa cells. However, these extracts also exhibited a cytotoxic effect against normal fibroblast 3T3 cells. Ethanolic fruit extract reduced the DNA synthesis of HeLa cells.

Conclusions: The active substances or preparations obtaining from the C. siliqua plant have the possibility of being an anticancer agent, whereas their cytotoxic effects on the normal cells should not be overlooked.

Anahtar Kelimeler

• HeLa cells,3T3 cells,Carob,Cytotoxicity,3T3 cells

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