Finite Element Analysis Of Electric Field For In-Vitro Electropermeabilization
Öz
Background: The aim of this study was to investigate
the electric field distribution inside the cell solution for
different electric pulse amplitudes.
Materials and Methods: Breast cancer cells were
loaded into a BTX 640 model cuvette with parallel
aluminum plate electrodes and the cuvette were placed
in the electroporation chamber which was connected to
electroporator. Eight square pulses of duration
100μs (having repetition frequency of 1Hz) with 400V
and 800V were applied to the electrodes. Since
electroporation involves electrostatic properties, its
analysis is performed using Ansys-Maxwell 3D
Electrostatic transient module. In this direction, it is
different from other studies in the literature. Firstly, the
capacitance of the cuvette is calculated analytically by
the parallel plate capacitor approach. Secondly; the
capacitance value is calculated numerically by the
software. The accuracy of the model is tested with
analytical and numerical analysis. Thirdly, the electric
field (E) distribution inside the cell solution was
examined.
Results: The capacitance (C) was calculated as
0.5461125pF by using the Formula. C value in the
numerical analysis was found to be 0.54387pF. The
electric field distribution was found around 1998.7-
2001,3V/cm. If E is too low, the potential value for
electroporation can not be reached. Increasing E at the
corners of the cell solution is an expected result.
Because electrical charges accumulate at corner points.
While the applied voltage is 400V, E value on the
solution is around
4 4
5.66 10 8.13 10
V/m. Thus,
the membrane potential is calculated at about 0.56-
0.81V. While the applied voltage is 800V, E is around
5 5
1.035 10 1.82 10
V/m on the solution. Thus,
the membrane potential is calculated at about 1.03 -
1.82 V.
Conclusion: The capacitance value error ratio between
analytical and numerical analysis was 0.047%. It is
expected that the actual model will be compatible with
the model in the simulation. As the amount of the cell
solution increased, a linear increase in the capacitance
value was observed. For this reason, the charging time
for electroporation of the cells is affected. In analyzes
performed with solution, when 400V is applied, the
permeability of the cells in the electric field values
(1000 V/cm) is low. However, increasing the voltage
value from 400V to 800V could significantly increase
the permeability of the cells.
Anahtar Kelimeler
Kaynakça
- 1.Ongaro A, Campana LG, De Mattei M, Dughiero F, Forzan M, Pellati A, Rossi CR, Sieni E. Evaluation of the Electroporation Efficiency of a Grid Electrode for Electrochemotherapy From Numerical Model to In Vitro Tests. Technology in cancer research &treatment 2015;15(2):296-307. 2.Cukjati D, Batiuskaite D, Slivnik T, Mir LM, Miklavčič D. Sequential finite element model of tissue electropermeabilization. IEEE Transactions on Biomedical Engineering 2005;52(5):816-827. 3.Dev SB, Rabussay DP, Widera G, Hofmann GA. Medical applications of electroporation. IEEE Transactions on Plasma Science 2000;28(1):206-23. 4.Rols MP, Golzio M, Gabriel B, Teissié J. Factors controlling electropermeabilisation of cell membranes. Technology in cancer research & treatment 2002;1(5):319- 27. 5.Castiello M, Dughiero F, Scandola F, Sieni E, Campana LG, Rossi CR, De Mattei M, Pellati A, Ongaro A. A new grid electrode for electrochemotherapy treatment of large skin tumors.IEEE Transactions on Dielectrics and Electrical Insulation 2014;21(3):1424-32. 6.Hong Z, Hao Z, Wei H, Zishu W, Qin G, Hong L.An exploration for optimal parameters of electromagnetic impulse on electrochemotherapy (ECT) of tumor.CEEM Proceedings2003;118-121 7.Esmekaya, M. A., Kayhan, H., Coskun, A., &Canseven, A. G.Effects of Cisplatin Electrochemotherapy on Human Neuroblastoma Cells. The Journal of membrane biology 2016;249(5):601-610. 8.Esmekaya, M. A., Kayhan, H., Yagci, M., Coskun, A., &Canseven, A. G. Effects of Electroporation on Tamoxifen Delivery in Estrogen Receptor Positive (ER+) Human Breast Carcinoma Cells. Cell biochemistry and biophysics 2017;75(1):103-109 9.Sree G, Velvizhi VK, Sundararajan R. Electric field distribution of malignant breast tissue under needle electrode configuration. InElectrical Insulation and Dielectric Phenomena (CEIDP) 2012:267-270 10.Campana, L. G., Dughiero, F., Forzan, M., Rossi, C. R., &Sieni, E. A prototype of a flexible grid electrode to treat widespread superficial tumors by meansof Electrochemotherapy. Radiology and oncology 2016; 50(1): 49-57. 11.Bommakanti S, Agoramurthy P, Campana L, Sundararajan R. A simulation analysis of large multielectrode needle arrays for efficient electrochemotherapy of cancer tissues.InElectrical Insulation and Dielectric Phenomena (CEIDP) 2011: 187-190 12.Miklavčič, D., Šemrov, D., Mekid, H., & Mir, L. M. A validated model of in vivo electric field distribution in tissues for electrochemotherapy and for DNA electrotransfer for gene therapy. BiochimicaetBiophysicaActa(BBA)- General Subjects 2000; 1523(1): 73-83. 13.Ansys Maxwell v16 Training Manual Lectures 4,5,6. 14.Ansys Maxwell v16 Help File. 15.Jaroszeski J. M., Heller R., Gilbert R. Electrochemptherapy, Electrogenetherapy and Transdermal Drug Delivery, Methods in Molecular Medicine, Humana Press, 2000:1-488
Ayrıntılar
Birincil Dil
İngilizce
Konular
-
Bölüm
Araştırma Makalesi
Yayımlanma Tarihi
30 Aralık 2017
Gönderilme Tarihi
29 Eylül 2017
Kabul Tarihi
22 Kasım 2017
Yayımlandığı Sayı
Yıl 2017 Cilt: 14 Sayı: 3