Şanlıurfa’daki Prostat Kanseri Hastalarında VGSC’lerin İmmünohistokimyasal Olarak İncelenmesi

Öz

Amaç: Bu çalışmada, genitoüriner sistem rahatsızlıkları nedeniyle Şanlıurfa'daki Harran Üniversitesi Tıp Fakültesi Hastanesi ve Mehmet Akif İnan Eğitim ve Araştırma Hastanesi’ne başvuran bazı hastalardan alınmış prostat doku örneklerinde Nav1.7 geni tarafından kodlanan proteinin varlığının araştırılması amaçlanmıştır. Materyal ve Metod: Çalışmada, tanı konulmuş (benign prostatik hiperplazi, prostatik intraepitelyal neoplazi, metastatik olmayan prostat kanseri ve metastatik prostat kanseri) 20 hastaya ait prostat dokusu örnekleri ve dört normal prostat kullanıldı. Harran Üniversitesi Tıp Fakültesi Hastanesi ile Mehmet Akif İnan Eğitim ve Araştırma Hastanesi'nin Patoloji arşivlerinden alınan bu numuneler 2012–2014 yıllarına aittir. İşaretleme için anti-sodyum kanalı Nav1.7 monoklonal antikor (Millipore, Merck, ABD) kullanıldı. Pozitif kontrol olarak, üretici firmanın önerisine göre, sıçan beyincik dokusu ile çalışıldı. İmmunohistokimyasal boyama işlemi otomatik immünhistokimya cihazında (Ventana, Roche, ABD) yapıldı. Bulgular: Primer antikor uygulanmayan negatif kontrol kesitlerinde boyanma olmadı. Pozitif kontrol ile karşılaştırıldığında prostat doku örneklerinde (normal prostat dokusu, benign prostatik hiperplazi, prostatik intraepitelyal neoplazi dokusu, metastatik olmayan prostat kanseri ve metastatik prostat kanseri) dikkate değer bir boyanma gerçekleşmediği gözlemlendi. Sonuç: Nav1.7 geni tarafından kodlanan VGSC proteini, normal prostat ve benign prostat hiperplazisi yanı sıra prostatik intraepitelyal neoplazi, metastatik olmayan prostat kanseri ve metastatik prostat kanseri gibi farklı derecelerde kanser bulunan hastalarda immünohistokimyasal olarak gösterilememiştir. İncelemeler, diğer genler tarafından kodlanan VGSC'lere karşı üretilmiş çeşitli antikorlarla işaretlenerek sürdürülmelidir. Ayrıca bulgular diğer moleküler tekniklerle de doğrulanmalıdır.

Anahtar Kelimeler

• Voltaj kapılı sodyum kanalları,Nav1.7 antikor,prostat kanseri,immunohistokimya

An Immunohistochemical Investigation of VGSCs in Prostate Cancer Patients in Sanliurfa

Öz

Aim: In this study, it was aimed to investigate presence of the protein which encoded by Nav1.7 gene in prostate tissue specimens obtained from some patients consulted to Harran University Medical Faculty Hospital and Mehmet Akif Inan Training and Research Hospital in Sanliurfa owing to genitourinary system disorders. Materials and Methods: In this study, it was used the prostate tissue samples which belong to 20 diagnosed patients (benign prostatic hyperplasia, prostatic intraepithelial neoplasia, non-metastatic prostate cancer and metastatic prostate cancer), and four normal prostate. These specimens obtained from Pathology Department archives of Harran University Faculty of Medicine Hospital and Mehmet Akif Inan Training and Research Hospital belong to 2012–2014. For labelling, Anti-Sodium channel Nav1.7 monoclonal antibody (Millipore, Merck, USA) was used. It was studied with rat cerebellum tissue as positive control in accordance with the manufacturer’s protocol. The immunohistochemical staining procedure was performed in automatic immunohistochemistry device (Ventana, Roche, USA). Results: There was no staining in the negative control sections which non-treated with primer antibody. No remarkable staining was observed in prostate tissue specimens (normal prostate tissue, benign prostatic hyperplasia, prostatic intraepithelial neoplasia tissue, non-metastatic prostate cancer and metastatic prostate cancer) when compared with the positive control. Conclusion: The VGSC protein which encoded by Nav1.7 gene was not showed by immunohistochemically in patients with normal prostate, benign prostatic hyperplasia as well as different grades of cancer such as prostatic intraepithelial neoplasia, non-metastatic prostate cancer, and metastatic prostate cancer. Investigations should be continued by marking with various antibodies that produced against VGSCs encoded by other genes. Additionally, the findings should confirm with other molecular techniques.

Anahtar Kelimeler

• Voltage-gated sodium channels,Nav1.7 antibody,prostate cancer,immunohistochemistry

Kaynakça

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