İnsan Umblikal Ven Endotel Hücre Kültüründe TNF- lfa İle Oluşturulmuş İn Vitro Greft Versus Host Hastalığı Modelinde Adrenomedullinin Sitoprotektif Etkisi

Öz

Giriş: Allojeneik kök hücre nakli (KHN) özellikle hematolojik malignitelerin tedavisinde önemli bir yer tutmaktadır. Greft versus host hastalığı (GVHH) allojeneik KHN'nin en sık görülen komplikasyonlarından biridir. Nakil sonrası GVHH'da temel patolojik mekanizmalardan birinin inflamatuar yolak üzerinden endotel hücre hasarı olduğu düşünülmektedir. İnflamatuar yolak üzerinden koruyucu etkisi olan adrenomedullin (AMD)'nin TNF-α ile oluşturulmuş in-vitro GVHH modelindeki sitoprotektif etkisi araştırıldı. 

Methods: İnsan umblikal veninden elde edilen endotel hücreleri TNF-α (grup1) ve TNF-α+AMD (grup2) ile iki saat inkübe edildi ve endotel hücre kaynaklı mikropartiküller (CD62, CD146, CD31, CD51/61) ve apoptozis belirteci olan Anneksin-Völçüldü. Bulgular: Her iki grupta endotelyal mikropartikül seviyeleri arasında fark yoktu. AMD sonrası bazı ölçümlerde artış tespit edilirken bazı ölçümlerde düşüş tespit edildi (p>0,05). Sonuç: In vitro akut GVHH modelinde AMD'in koruyucu etkisi olup olmadığıni tespit etmek için çok ölçümlü ve büyük çalışmalara ihtiyaç vardır. 

Anahtar Kelimeler

• Adrenomedullin,greft versus host hastalığı,mikropartikül

Effects Of Adrenomedullin On Endothelial Microparticle Release In TNF-Αlpha Stimulated In-Vitro Graft Versus Host Disease Model

Öz

Background: Allogeneic stem cell transplantation (SCT) is an important treatment modality used in hematological malignant disorders. Graft-versus-host disease (GVHD) is the main complication after allogeneic SCT. It is suggested that endothelial cell damage has an important role in development of GVHD. In this study we investigated the effect of adrenomedullin (AMD) in TNF-α stimulated in-vitro GVHD model since AMD has a protective effect on cells via the regulation inflammatory pathway. Materials and Methods: Umbilical cord derived endothelial cells were incubated with TNF-α for 2 hours in group1 and with TNF-α+AMD in group2 in order to make in-vitro GVHD model and see the effect of AMD. Microparticles (CD62, CD146, CD31, CD51/61) originated from endothelial cells that are the markers of injury or apoptosis were measured. Results: We found no significant difference between the microparticle levels in both groups. Although our hypothesis was to find decrement in microparticle levels in gruop2 there were increases and decreases in microparticle levels in both groups (p>0,05). Conclusion: To detect whether there is a protective effect of AMD on development of in-vitro acute GVHD model extensive studies with multiple measurements are needed. 

Anahtar Kelimeler

• Adrenomedullin,graft versus host disease,microparticle

Kaynakça

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