Impacts of radio-frequency electromagnetic field from mobile phone exposure on apoptosis

Öz

Abstract

 

Background: The use of mobile phones is becoming more widespread and the concerns about possible harmful effects are increasing in the community. The RF electromagnetic fields have been declared as carcinogen class 2B by the International Agency for Research on Cancer, an organization affiliated to the World Health Organization. In this study, we examined effects of radio-frequency electromagnetic field (RF-EMF) from mobile phone exposure on apoptosis and cell viability were investigated.

Methods: Human astrocytoma cell lines, were exposed to RF-EMF at mobile phone frequency for 1, 24, and 48 h.  Apoptotic rates of experimental groups were examined by annexin V-FITC/PI assay in flow cytometry. Additionally cell viability was determined by using the wst-1 assay with elisa reader.

Results: The experimental results show that RF-EMF exposure with 1 and 24 hours has no significant effect on cell viability and apoptosis in astrocytoma cells. Conversely, 48-hour RF field exposure statistically significantly decreased cell viability and increased apoptosis in astrocytoma cells.

Conclusions: The results of the experiment show that doubling time of cells is important in observing RF EMF induced effects.

 

Anahtar Kelimeler

• Keywords: Mobile phone,Radiofrequency,Astrocytoma,Apoptosis

Cep telefonu maruziyetinden kaynaklanan Radyofrekans elektromanyetik alanın apoptoz üzerine etkisi

Öz

Amaç: Cep telefonu kullanımı her geçen gün daha da yaygınlaşmakta olup, olası sağlık etkileri ile ilgili endişeler toplum arasında artmaktadır. Dünya Sağlık Örgütü'ne bağlı bir kuruluş olan "Uluslararası Kanser Araştırmaları Ajansı" tarafından RF elektromanyetik alanlar 2B sınıfı karsinojen olarak ilan edilmiştir. Bu çalışmada, cep telefonu kaynaklı RF elektromanyetik alan (RF-EMF) maruziyetinin, apoptoz ve hücre canlılığı üzerindeki etkileri araştırılmıştır.

Materyal ve metod: U118-MG insan astrositoma hücreleri, 2.1 GHz cep telefonu frekanslı RF-EMF’e 1, 24 ve 48 saat süreler ile maruz bırakıldı. Deney gruplarındaki apoptoz oranları akım sitometride anneksin V-FITC/PI yöntemi kullanılarak belirlenmiştir. Hücre canlılığı ise mikroplaka okuyucuda wst-1 yöntemi kullanılarak tespit edilmiştir.

Bulgular: Deney sonuçları, 2.1 GHz frekanslı RF-EMF’e 1 ve 24 saat süre ile maruz bırakılan deney gruplarında hücre canlılığı ve apoptozda önemli bir etki gözlenmemiştir. Bunun aksine 48 saatlik RF alan maruziyeti hücre canlılığında istatistiksel olarak anlamlı bir azalmaya sebep olurken, apoptozda yine istatistiksel olarak anlamlı bir artışa neden olduğu tespit edilmiştir.

Sonuç: Deney sonuçları RF EMF kaynaklı etkilerin gözlenmesinde hücrelerin ikiye katlanma sürelerinin önemli olduğunu göstermektedir.

Anahtar Kelimeler

• Cep telefonu,Radyofrekans,Astrositoma,Glioblastoma,apoptoz

Kaynakça

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