Ratlarda Deneysel Olarak Oluşturulan Renal İskemi-Reperfüzyon Hasarında Likopen ve Kafeinin Antioksidan Etkilerinin İncelenmesi

Öz

Amaç: Renal iskemi/reperfüzyon hasarına maruz kalmış ratlarda likopen ve kafeinin antioksidan etkilerinin incelenmesi amaçlandı. Materyal ve Metod: Çalışmada kullanılan 200-250 gram Wistar albino cinsi erkek ratlara sağ renal nefroktomi sonrası 60 dk iskemi ve bunu takiben 60 dk reperfüzyon gerçekleştirildi. İskemiden 30 dk önce ratlara Likopen (5 mg/kg), kafein (50 mg/kg), Likopen (5 mg/kg) + kafein (50 mg/kg) intraperitoneal olarak uygulandı. Deney sonrası ratlardan 4 ml kan ve sol böbrek alındı. Biyokimyasal olarak doku malondialdehit, glutatyon peroksidaz, süperoksit dismutaz, serum kreatinin aktiviteleri ölçüldü ve histolojik olarak ışık mikroskobi bulguları değerlendirildi. Bulgular: Likopen ve kafeinin renal iskemi reperfüzyon hasarına karşı antioksidan etkisi diğer gruplarla karşılaştırıldığında likopenin serum kreatinin seviyelerinde anlamlı bir düşüş gözlenmiştir. Kafein ise yüksek çıkmakla birlikte anlamlı değildir. Gruplarda malondialdehit seviyeleri hasar grubuna göre düşük çıkmıştır, glutatyon peroksidaz ve süperoksit dismutaz ise yüksek olmasına rağmen anlamlılık gözlenmemiştir. Histopatolojik hasar; iskemi-reperfüzyon grubuyla karşılaştırıldığında diğer gruplarda anlamlı derecede düşük çıkmıştır. Likopen ve kafein; apoptozis, tübüler dilatasyon, tübüler epitel dejenerasyonu, glomerüler büzülme, deskuame epitel ve proteinöz kast miktarını önemli ölçüde düşürmüştür. Sonuç: Likopenin kafeine göre antioksidan etkinliği yüksek olmakla birlikte likopen ve kafeinin renal iskemi reperfüzyon hasarına karşı koruyucu etkisi vardır.

Anahtar Kelimeler

• Böbrek
• İskemi-Reperfüzyon Hasarı
• Likopen
• Kafein

Analysis of Antioxidant Effects of Lycopene and Caffeine in Experimentally Induced Renal Ischemia-Reperfusion Injury in Rats

Öz

Background: The aim was to investigate the antioxidant effects of lycopene and caffeine in rats exposed to renal ischemia/reperfusion injury. Materials and Methods: Following right renal nephrectomy, 60 minutes of ischemia followed by 60 minutes of reperfusion were performed in 200-250 gram Wistar albino rats used in the study. 30 minutes before ischemia, lycopene (5 mg/kg), caffeine (50 mg/kg), lycopene (5 mg/kg) + caffeine (50 mg/kg) were administered intraperitoneally to the rats. After the experiment, 4 ml of blood and left kidney were taken from the rats. Tissue malondialdehyde, glutathione peroxidase, superoxide dismutase and serum creatinine activities were measured biochemically and light microscopy findings were evaluated histologically. Results: When the antioxidant effect of lycopene and caffeine against renal ischemia perfusion injury was compared with the other groups, a significant decrease was found in serum creatinine levels of lycopene. Caffeine was found to be high, but not statistically. malondialdehyde levels were lower in the groups than in the injury group; although glutathione peroxidase and superoxide dismutase were high, no significance was found. When compared with ischemia-reperfusion group, histopathological damage was found to be significantly lower than in the other groups. It was found that lycopene and caffeine reduced apoptosis, tubular dilatation, tubular epithelium degeneration, glomerular shrinkage, desquame epithelium and proteinaceous cast amount significantly. Conclusions: While lycopene has high antioxidant efficiency when compared with caffeine, lycopene and caffeine have protective effect against renal ischemia reperfusion injury.

Anahtar Kelimeler

• Kidney
• Ischemia-Reperfusion Injury
• Lycopene
• Caffeine

Kaynakça

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