Impacts of radio-frequency electromagnetic field from mobile phone exposure on apoptosis

Yıl 2019, Cilt: 16 Sayı: 1, 123 - 129, 22.03.2019

Mehmed Zahid Tüysüz Handan Kayhan Atiye Seda Yar Sağlam Emin Umit Bağrıaçık Munci Yağcı Ayse Gulnihal Canseven

Öz

Abstract

 

Background: The use of mobile phones is becoming more
widespread and the concerns about possible harmful effects are increasing in
the community. The RF electromagnetic fields have been declared as carcinogen
class 2B by the International Agency for Research on Cancer, an organization
affiliated to the World Health Organization. In this study, we examined effects
of radio-frequency electromagnetic field (RF-EMF) from mobile phone exposure on
apoptosis and cell viability were investigated.

Methods: Human astrocytoma cell lines, were exposed to
RF-EMF at mobile phone frequency for 1, 24, and 48 h.  Apoptotic rates of experimental groups were
examined by annexin V-FITC/PI assay in flow cytometry. Additionally cell
viability was determined by using the wst-1 assay with elisa reader.

Results: The experimental results show that RF-EMF
exposure with 1 and 24 hours has no significant effect on cell viability and
apoptosis in astrocytoma cells. Conversely, 48-hour RF field exposure
statistically significantly decreased cell viability and increased apoptosis in
astrocytoma cells.

Conclusions: The results of the experiment show that
doubling time of cells is important in observing RF EMF induced effects.

 

Anahtar Kelimeler

Keywords: Mobile phone, Radiofrequency, Astrocytoma, Apoptosis

Cep telefonu maruziyetinden kaynaklanan Radyofrekans elektromanyetik alanın apoptoz üzerine etkisi

Öz

Amaç: Cep
telefonu kullanımı her geçen gün daha da yaygınlaşmakta olup, olası sağlık
etkileri ile ilgili endişeler toplum arasında artmaktadır. Dünya Sağlık
Örgütü'ne bağlı bir kuruluş olan "Uluslararası Kanser Araştırmaları
Ajansı" tarafından RF elektromanyetik alanlar 2B sınıfı karsinojen olarak
ilan edilmiştir. Bu çalışmada, cep telefonu kaynaklı RF elektromanyetik alan (RF-EMF)
maruziyetinin, apoptoz ve hücre canlılığı üzerindeki etkileri araştırılmıştır.

Materyal ve metod: U118-MG
insan astrositoma hücreleri, 2.1 GHz cep telefonu frekanslı RF-EMF’e 1, 24 ve
48 saat süreler ile maruz bırakıldı. Deney gruplarındaki apoptoz oranları akım
sitometride anneksin V-FITC/PI yöntemi kullanılarak belirlenmiştir. Hücre
canlılığı ise mikroplaka okuyucuda wst-1 yöntemi kullanılarak tespit
edilmiştir.

Bulgular: Deney
sonuçları, 2.1 GHz frekanslı RF-EMF’e 1 ve 24 saat süre ile maruz bırakılan deney
gruplarında hücre canlılığı ve apoptozda önemli bir etki gözlenmemiştir. Bunun
aksine 48 saatlik RF alan maruziyeti hücre canlılığında istatistiksel olarak
anlamlı bir azalmaya sebep olurken, apoptozda yine istatistiksel olarak anlamlı
bir artışa neden olduğu tespit edilmiştir.

Sonuç: Deney
sonuçları RF EMF kaynaklı etkilerin gözlenmesinde hücrelerin ikiye katlanma
sürelerinin önemli olduğunu göstermektedir.

Anahtar Kelimeler

Cep telefonu, Radyofrekans, Astrositoma, Glioblastoma, apoptoz

Kaynakça

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