BibTex RIS Kaynak Göster

Akut lenfoblastik lösemili çocuk hastaların beyin omurilik sıvısı oksidan/antioksidan seviyeleri

Yıl 2014, Cilt: 11 Sayı: 2, 102 - 110, 01.08.2014

Öz

Amaç: Akut lenfoblastik lösemili çocuk hastalarda tedavinin beyin omurilik sıvısında BOS antioxidant ve oksidan seviyelerini araştırmak.Materyal ve Metod: 24 15 erkek 9 kız yeni tanı almış hastaların tanı anında, remisyon indüksiyon, güçlendirme, idame ve tedavi sonunda BOS seruloplazmin ferroksidaz aktivitesi, total tiyol, lipid hidroperoksit LOOH , ve total oksidant seviyeleri TOS çalışıldı. Çalışmaya 19 çocuk 10 erkek 9 kız kontrol olarak alındı. Bulgular: İlk tanıda ALL grubunda kontrol grubuna göre sırasıyla, ortalama ferrokisdaz aktivitesi 50.2 ± 10.4 U/L ve 30.3 ± 7.9 U/L , total tiyol 0.34 ± 0.02 µmol/L ve 0.18 ± 0.01 µmol/L , LOOH 6.5 ± 1.9 µmol/L ve 5.3 ± 0.8 µmol/L , ve TOS 9.3 ± 4.3 µmol H O equiv./L ve 6 ± 1.5 H O equiv./L bulundu P < 0.05 . Ferroksidaz aktivitesi tüm tedavi süresince yüksek seyrettiği, total tiyol seviyesinin ise tedavinin ilk 80 haftasında yüksek kaldığı daha sonra azlamaya başladığı, total oxidant seviyenin ise idamenin 7 haftası ile 120 haftasında düşüşler gösterdiği saptandı.Sonuç: ALL'li çocuk hastaların BOS oksidan stresi ve antioksidanları yüksek seviyededir ve tedavi süresince bu yükseklik devam etmektedir

Kaynakça

  • ) Pui CH, Mullighan CG, Evans WE, Relling MV. Pediatric acute lymphoblastic leukemia: where are we g o i n g a n d h o w d o w e g e t t h e r e ? B l o o d ;120(6):1165-74.
  • ) Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and h u m a n d i s e a s e . I n t J B i o c h e m C e l l B i o l ;39(1):44–84. ) Masutani H. Oxidative stress response and signaling in hematological malignancies and HIV infection. Int J Hematol 2000;71(1):25-32.
  • ) Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative s t r e s s - i n d u c e d c a n c e r. C h e m B i o l I n t e r a c t ;160(1):1–40. ) Al-Tonbary Y, Al-Hasan SA, Zaki M et al. Impact of anti-oxidant status and apoptosis on the induction phase of chemotherapy in childhood acute lymphoblastic leukemia. Hematology 2011;16(1):14-9.
  • ) Protas PT, Muszynska-Roslan K, Holownia A, Krawczuk-Rybak M, Braszko JJ. Cerebrospinal fluid oxidative stress during chemotherapy of acute lymphoblastic leukemia in children. Pediatr Hematol Oncol 2010;27(4):306-13.
  • ) Zelen I, Djurdjevic P, Popovic S, et al. Antioxidant enzymes activities and plasma levels of oxidative stress markers in B-chronic lymphocytic leukemia patients. J BUON 2010;15(2):330-6.
  • ) Drabko K, Bojarska-Junak A, Kowalczyk J. Activity of superoxide dismutase and glutathione peroxidase and concentrations of malonyldialdehyde, vitamin E, total antioxidant status and extracellular cytokines concentrations in children with acute lymphoblastic leukaemia (ALL). Med Wieku Rozwoj 2006;10:861-8.
  • ) Yetgin S, 18. Gürgey A, Tuncer AM et al. A c o m p a r i s o n o f t h e e f f e c t o f h i g h - d o s e m e t h y l p r e d n i s o l o n e w i t h c o n v e n t i o n a l - d o s e prednisolone in acute lymphoblastic leukemia patients with randomization. Leuk Res. 1998;22(6):485-93.
  • ) Pui CH, Campana D, Pei D et al. Treating childhood acute lymphoblastic leukemia without cranial irradiation. N Engl J Med 2009;360(26):2730–41.
  • ) Pui CH, Mahmoud HH, Rivera GK et al. Early intensification of intrathecal chemotherapy virtually eliminates central nervous system relapse in children with acute lymphoblastic leukemia. Blood 1998;92(2):411–5.
  • ) Erel O. Automated measurement of serum ferroxidase activity. Clin Chem 1998;44(11):2313–9. ) Erel O
  • A new automated colorimetric method for measuring total oxidant status. Clin Biochem ;38(12):1103-11.
  • ) Hu ML, Louie S, Cross CE, Motchnik P, Halliwell B. Antioxidant protection against hyochlorous acid in human plasma. J Lab Clin Med 1993;121(2):257-62.
  • ) Arab K, Steghens JP. Serum lipid hydroperoxides measurement by an automated xylenol orange method. Anal Biochem 2004;325(1):158-63.
  • ) Devi GS, Prasad MH, Saraswathi I, at al. Free radicals antioxidant enzymes and lipid peroxidation in different types of leukemias. Clin Chim Acta 2000;293(1-2):53-62.
  • ) Aycicek A, Iscan A, Erel O, Akcali M, Selek S. Total Antioxidant/Oxidant Status in Meningism and Meningitis. Pediatr Neurol 2006;35(6):382-6.
  • ) Ray G, Aneja S, Jain M, Batra S. Evaluation of free radical status in cerebrospinal fluid in childhood meningitis. Ann Trop Paediatr. 2000;20(2):115-20.
  • ) Neyestani TR, Fereydouni Z, Hejazi S, et al. Vitamin C status in Iranian children with acute lymphoblastic leukemia: evidence for increased utilization. J Pediatr Gastroenterol Nutr 2007;45(1):141-4.
  • ) Osterlundh G, Kjellmer I, Lannering B, et al. Neurochemical markers of brain damage in cerebrospinal fluid during induction treatment of acute lymphoblastic leukemia in children. Pediatr Blood Cancer 2008;50(4):793-8.
  • ) Hamerschlak N. Leukemia: genetics and prognostic factors. J Pediatr 2008;84(4S):52-7.
  • ) Pui CH, Relling MV, Downing JR. Acute lymphoblastic leukemia. N Engl J Med 2004; (15):1535–48.
  • ) Winick NJ, Bowman WP, Kamen BA, et al. Unexpected acute neurologic toxicity in the treatment of children with acute lymphoblastic leukemia. J Natl Cancer Inst 1992;84(4):252–6.
  • ) Nakagawa K. Effect of chemotherapy on ascorbate and ascorbyl radical in cerebrospinal fluid and serum of acute lymphoblastic leukemia. Cell Mol Biol (Noisy-le- grand) 2000; 46(8):1375-81.
  • ) Zhou Y, Hileman EO, Plunkett W, Keating MJ, Huang P. Free radical stress in chronic lymphocytic leukemia cells and its role in cellular sensitivity to ROS- g e n e r a t i n g a n t i c a n c e r a g e n t s . B l o o d ;101(101):4098–104.
  • ) Kocyigit A, Keles H, Selek S, et al. Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis. Mutat Res 2005;585(1-2):71–8.
  • ) Natesha RK, Natesha R, Victory D, Barnwell SP, Hoover EL. A prognostic role for ceruloplasmin in the diagnosis of indolent and recurrent inflammation. J Natl Med Assoc 1992;84(9):781–4.

Cerebrospinal fluid oxidant/antioksidant status in children with acute lymphoblastic leukemia

Yıl 2014, Cilt: 11 Sayı: 2, 102 - 110, 01.08.2014

Öz

Objective: To assess the effect of leukemia therapy on the cerebrospinal fluid CSF antioxidant and oxidant status in children with acute lymphoblastic leukemia ALL .Material and Method: Twenty-four consecutive children 15 boys and 9 girls newly diagnosed with ALL were enrolled in the study. CSF concentrations of ceruloplasmin ferroxidase activity, total thiol group, lipid hydroperoxide LOOH and total oxidant status TOS were evaluated at diagnosis, remission induction, consolidation, continuation, and the end of the continuation. Nineteen children 10 boys and 9 girls were enrolled as a reference group. Results: The study group at diagnosis had significant higher mean ferroxidase activity 50.2 ± 10.4 U/L and 30.3 ± 7.9 U/L , total thiol 0.34 ± 0.02 µmol/L and 0.18 ± 0.01 µmol/L , LOOH 6.5 ± 1.9 µmol/L and 5.3 ± 0.8 µmol/L , and TOS 9.3 ± 4.3 µmol H O equiv./L and 6 ± 1.5 H O equiv./L levels compared with the references, respectively P < 0.05 .. Ferroxidase activity remained elevated during the entire treatment course. Total thiol levels stayed elevated from diagnosis to week 80 of ALL treatment and significantly decreased thereafter. LOOH levels were progressively increased to week 80 of the treatment course. TOS levels were progressively decreased two times at continuation week 7 and end of the treatment. Conclusions: Our data indicate that CSF oxidative stress and antioxidants are higher at diagnosis, significant changes to be during therapy, and is already high at the end of therapy in children with ALL

Kaynakça

  • ) Pui CH, Mullighan CG, Evans WE, Relling MV. Pediatric acute lymphoblastic leukemia: where are we g o i n g a n d h o w d o w e g e t t h e r e ? B l o o d ;120(6):1165-74.
  • ) Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and h u m a n d i s e a s e . I n t J B i o c h e m C e l l B i o l ;39(1):44–84. ) Masutani H. Oxidative stress response and signaling in hematological malignancies and HIV infection. Int J Hematol 2000;71(1):25-32.
  • ) Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative s t r e s s - i n d u c e d c a n c e r. C h e m B i o l I n t e r a c t ;160(1):1–40. ) Al-Tonbary Y, Al-Hasan SA, Zaki M et al. Impact of anti-oxidant status and apoptosis on the induction phase of chemotherapy in childhood acute lymphoblastic leukemia. Hematology 2011;16(1):14-9.
  • ) Protas PT, Muszynska-Roslan K, Holownia A, Krawczuk-Rybak M, Braszko JJ. Cerebrospinal fluid oxidative stress during chemotherapy of acute lymphoblastic leukemia in children. Pediatr Hematol Oncol 2010;27(4):306-13.
  • ) Zelen I, Djurdjevic P, Popovic S, et al. Antioxidant enzymes activities and plasma levels of oxidative stress markers in B-chronic lymphocytic leukemia patients. J BUON 2010;15(2):330-6.
  • ) Drabko K, Bojarska-Junak A, Kowalczyk J. Activity of superoxide dismutase and glutathione peroxidase and concentrations of malonyldialdehyde, vitamin E, total antioxidant status and extracellular cytokines concentrations in children with acute lymphoblastic leukaemia (ALL). Med Wieku Rozwoj 2006;10:861-8.
  • ) Yetgin S, 18. Gürgey A, Tuncer AM et al. A c o m p a r i s o n o f t h e e f f e c t o f h i g h - d o s e m e t h y l p r e d n i s o l o n e w i t h c o n v e n t i o n a l - d o s e prednisolone in acute lymphoblastic leukemia patients with randomization. Leuk Res. 1998;22(6):485-93.
  • ) Pui CH, Campana D, Pei D et al. Treating childhood acute lymphoblastic leukemia without cranial irradiation. N Engl J Med 2009;360(26):2730–41.
  • ) Pui CH, Mahmoud HH, Rivera GK et al. Early intensification of intrathecal chemotherapy virtually eliminates central nervous system relapse in children with acute lymphoblastic leukemia. Blood 1998;92(2):411–5.
  • ) Erel O. Automated measurement of serum ferroxidase activity. Clin Chem 1998;44(11):2313–9. ) Erel O
  • A new automated colorimetric method for measuring total oxidant status. Clin Biochem ;38(12):1103-11.
  • ) Hu ML, Louie S, Cross CE, Motchnik P, Halliwell B. Antioxidant protection against hyochlorous acid in human plasma. J Lab Clin Med 1993;121(2):257-62.
  • ) Arab K, Steghens JP. Serum lipid hydroperoxides measurement by an automated xylenol orange method. Anal Biochem 2004;325(1):158-63.
  • ) Devi GS, Prasad MH, Saraswathi I, at al. Free radicals antioxidant enzymes and lipid peroxidation in different types of leukemias. Clin Chim Acta 2000;293(1-2):53-62.
  • ) Aycicek A, Iscan A, Erel O, Akcali M, Selek S. Total Antioxidant/Oxidant Status in Meningism and Meningitis. Pediatr Neurol 2006;35(6):382-6.
  • ) Ray G, Aneja S, Jain M, Batra S. Evaluation of free radical status in cerebrospinal fluid in childhood meningitis. Ann Trop Paediatr. 2000;20(2):115-20.
  • ) Neyestani TR, Fereydouni Z, Hejazi S, et al. Vitamin C status in Iranian children with acute lymphoblastic leukemia: evidence for increased utilization. J Pediatr Gastroenterol Nutr 2007;45(1):141-4.
  • ) Osterlundh G, Kjellmer I, Lannering B, et al. Neurochemical markers of brain damage in cerebrospinal fluid during induction treatment of acute lymphoblastic leukemia in children. Pediatr Blood Cancer 2008;50(4):793-8.
  • ) Hamerschlak N. Leukemia: genetics and prognostic factors. J Pediatr 2008;84(4S):52-7.
  • ) Pui CH, Relling MV, Downing JR. Acute lymphoblastic leukemia. N Engl J Med 2004; (15):1535–48.
  • ) Winick NJ, Bowman WP, Kamen BA, et al. Unexpected acute neurologic toxicity in the treatment of children with acute lymphoblastic leukemia. J Natl Cancer Inst 1992;84(4):252–6.
  • ) Nakagawa K. Effect of chemotherapy on ascorbate and ascorbyl radical in cerebrospinal fluid and serum of acute lymphoblastic leukemia. Cell Mol Biol (Noisy-le- grand) 2000; 46(8):1375-81.
  • ) Zhou Y, Hileman EO, Plunkett W, Keating MJ, Huang P. Free radical stress in chronic lymphocytic leukemia cells and its role in cellular sensitivity to ROS- g e n e r a t i n g a n t i c a n c e r a g e n t s . B l o o d ;101(101):4098–104.
  • ) Kocyigit A, Keles H, Selek S, et al. Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis. Mutat Res 2005;585(1-2):71–8.
  • ) Natesha RK, Natesha R, Victory D, Barnwell SP, Hoover EL. A prognostic role for ceruloplasmin in the diagnosis of indolent and recurrent inflammation. J Natl Med Assoc 1992;84(9):781–4.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makalesi
Yazarlar

Ali Ayçiçek Bu kişi benim

Ahmet Koç Bu kişi benim

Yeşim Oymak Bu kişi benim

Bülent Adar Bu kişi benim

Süleymen Geter Bu kişi benim

Abdullah Solmaz Bu kişi benim

Yayımlanma Tarihi 1 Ağustos 2014
Yayımlandığı Sayı Yıl 2014 Cilt: 11 Sayı: 2

Kaynak Göster

Vancouver Ayçiçek A, Koç A, Oymak Y, Adar B, Geter S, Solmaz A. Akut lenfoblastik lösemili çocuk hastaların beyin omurilik sıvısı oksidan/antioksidan seviyeleri. Harran Üniversitesi Tıp Fakültesi Dergisi. 2014;11(2):102-10.

Harran Üniversitesi Tıp Fakültesi Dergisi  / Journal of Harran University Medical Faculty