E vitamini ve selenyumun deneysel diyabette trombosit ve eritrosit indeksleri ile pankreas histopatolojisi üzerine etkisi Türkçe

Yıl 2013, Cilt: 10 Sayı: 2, 54 - 59, 01.08.2013

Muhammed Yaşar Dörtbudak Mehmet Şevki Çadırcı Ali Ziya Karakılçık

Öz

Amaç: Bu çalışmada, deneysel diyabette E vitamini ve Selenyum Se 'un eritrosit ve trombosit indeksleri ile pankreas dokusu üzerinde olası etkileri araştırılmıştır. Materyal ve metot: Çalışma beş gruba ayrılan Wistar albino ratlar üzerinde yürütüldü. Birinci gruptaki ratlar n=5 kontrol olarak kullanıldı, bunlara sadece yem ve su verildi. İkinci gruptaki ratlarda n=5 65 mg/kg 0,1 M sitrat tamponda hazırlanmış streptozotosin STZ intraperitoneal İP uygulanarak deneysel diyabet oluşturuldu. STZ enjeksiyonuna ilaveten, üçüncü gruba n=10 E vitamini alfa-tokoferol asetat, 100 mg/kg , dördüncü gruba n=10 Se sodyum selenat 0.3 mg/kg , beşinci gruba n=10 ise Se-E vitamini kombinasyonu sodyum selenat 0.3 mg/kg+alfa-tokoferol asetat, 100 mg/kg gün aşırı uygulandı. Onbeş gün süren çalışma sonunda tüm ratlardan kan alınarak hematolojik değerler belirlendi. Pankreastan alınan doku kesitleri histopatolojik olarak incelendi. Bulgular: Tüm gruplarda alyuvar sayısı, hemoglobin miktarı, hematokrit değer ile alyuvar hacmi, ortalama alyuvar hemoglobini, ortalama alyuvar hemoglobin yoğunluğu, alyuvar dağılım genişliği gibi alyuvar indeksleri; trombosit sayısı, ortalama trombosit hacmi, trombokrit/plateletkrit değeri ve trombosit dağılım genişliği gibi trombosit indeksleri ile akyuvar sayısı, nötrofil, lenfosit, monosit, eozinofil, bazofil sayıları ve bu lökositlerin oranları % otomatik cell-counter'de belirlendi. Sonuç: Se ve E vitamininin deneysel diyabette trombosit ve trombokrit/plateletkrit değerleri ile nötrofil, eosinofil, bazofil sayıları üzerinde etkili olduğu saptandı. Ancak diğer kan parametreleri üzerindeki etkilerinin istatistiksel anlamda önemli olmadığı, kontrol grubuna göre deneme gruplarında ışık mikroskobisi düzeyinde pankreas kesitlerinde önemli bir değişiklik oluşmadığı gözlemlendi

Anahtar Kelimeler

Pankreas, eritrosit ve trombosit indeksleri, diyabet, rat

Effects of vitamin E and selenium on erythrocyte and platelet indices and pancreatic histopathology in experimental diabetes

Öz

Background: In this study, possible effects of Se and vitamin E were investigated on histopathology of pancreas, platelet and erythrocyte indices in experimental diabetes.Methods: All Wistar albino rats were randomly divided into five groups. The first group n=5 was used as a control and this group was received only food and water. The second group n=5 was used as diabetic control group and it was only made diabetic by intraperitoneal injection in one time with 65 mg/kg of streptozotosin STZ in citrate buffer. The third group n=10 received with Se Na SeO , 0.3 mg/kg body weight , the fourth group n=10 with vitamin E dl--tocopheryl acetate, 100 mg/kg body weight , and the fifth group n=10 with Se plus vitamin E combination Na SeO , 0.3 mg/kg body weight+dl--tocopheryl acetate, 100 mg/kg body weight for every other day intervals during fifteen days. Blood parameters of the all rats were analyzed in 15 days after STZ injection. Then, their pancreatic tissues were histopathologically examined.Results: Platelet indices such as platelet counts, mean platelet volume, plateletcrit and platelet distribution width, erythrocyte indices such as red blood cell conunts, hemoglobin, heamatocrit%, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width and also, the values of white blood cell, neutrophil, lymphocyte, monocyte, eosinophil and basophil and percentage of these leukocytes % in blood samples of all groups were determined by automotic cellcounter. Conclusions: As a result, it was determined that Se and vitamin E affected the values of plateletcrit neutrophil, eosinophil and hematological parameters of these antioxidants. In the histopathologic results, we couldn't see any change or important effect on the pancreas tissue on the light microscope in all experimental groups.platelet and basophil There were not statistical changes on the values of other

Anahtar Kelimeler

Pancreas, erythrocyte and platelet indices, diabetes mellitus, rat

Kaynakça

• ) Aksoy N, Vural H, Sabuncu T, Aksoy S. Effects of melatonin on oxidative-antioxidative status of tissues in streptozotocin-induced diabetic rats. Cell Biochem Func 2003; 21(2): 121–5.
• ) Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes 1991; 40(4): 405–12.
• ) Konukoglu D, Kemerli GD, Sabuncu T, Hatemi H. Relation of erythrocyte Na+-K+ ATPase activity and cholesterol and oxidative stress in patients with type 2 diabetes mellitus. Clin Invest Med 2003; 26(6): 279–84.
• ) Styskal JL, Remmen HV, Richardson A, Salmon AB. Oxidative stress and diabetes: What can we learn about insulin resistance from antioxidant mutant mouse models? Free Radic Biol Med 2012; 52(1): 46–58.
• ) Vural H, Sabuncu T, Arslan SO, Aksoy N. Melatonin inhibits lipid peroxidation and stimulates the antioxidant status of diabetic rats. J Pineal Res 2001; 31(3): 193–8.
• )Combs GF, Combs SB. The Nutritional Biochemistry of Selenium. Ann Rev Nutr 1984; 4: –80.
• ) Karakılçık AZ, Aksakal M. Selenyumun bazı fizyolojik işlevleri, metabolizması ve E vitamini ile arasındaki ilişkiler. Gaziantep Tıp Derg 1993; 4: –91.
• ) Kutsky RJ. Handbook of vitamins. minerals and hormones. 2th ed. VNR. New York, 1981; –207.
• ) Linder MC. Nutritional biochemistry and metabolism in clinical applications. California State University, 1991; 167–75.
• ) Mc Dowell LR. Vitamins in animal nutrition comperative aspects to human nutrition. Academic Pres, 1989; 93–106.
• ) Iizuka Y, Sakurai E, Hikichi N. Effects of selenium on the serum glucose and insulin levels in diabetic rats. Nihon Yakurigaku Zasshi 1992; (2): 151–6.
• ) McNeill JH, Delgotti HLM, Battel ML. Insulinelike effects of sodium selenate in streptozotosin induced diyabetic rats. Diyabetes ; 40(12): 1675–8.
• ) Ghaffari T, Nouri M, Irannejad E, Rashidi MR. Effect of vitamin E and selenium supplement on paraoxanase-1 activity, oxydized low density lipoprotein and antioxidant defense in diabetic rats. BioImpacts 2011; 1(2): 121–8.
• ) Nazıroğlu M, Çay M. Protective role of intraperitonelly administered vitamin E and selenium on the antioxidative defense mechanisms in rats with diabetes induced by streptozotocin. Biol Trace Elem Res 2001; 79(2): –59.
• ) Packer L. The role of antioxidative treatment in diabetes mellitus. Diyabetologia 1993; 36(11): –3.
• ) Pazdro R, Burgess JR. The role of vitamin E and oxidative stress in diabetes complications. Mech Ageing Dev. 2010; 131(4): 276–86.
• ) Büyükdevrim S. Diyabetus Mellitus. İstanbul Üniversitesi Sağlık Bilimleri Enstitüsü Yayınları, ; 187–8. ) Beyazit Y, Sayilir A, Torun S, Suvak B, Yeşil Y, Purnak T, et al. Mean platelet volume as an indicator of disease severity in patients with acute pancreatitis. Clin Res Hepatol Gastroenterol. ; 36(2): 162-8. ) Hekimsoy Z, Payzin B, Talat O, Kandogan G. Mean platelet volume in Type-2 diabetic patients. J Diabetes Complications. 2004; 18(3): 173-6.
• ) Mazzanfi L, Mutus B. Diabetes-induced alterations in platelet metabolism. Clin Biochem ; 30 (7): 509–15. ) Jindal S, Gupta S, Gupta R, Kakkar A, Singh HV, Gupta K, et al. Platelet indices in diabetes mellitus: indicators of diabetic microvascular complications. Hematology 2011; 16(2): 86–9.
• ) Bath PM, Butterworth RJ. Platelet size: measurement, physiology and vascular disease. Blood Coagul Fibrinolysis 1996; 7(2): 157–61.
• ) Baynes RD, Lamparellı RDV, Chetty N, Atkınson P, Bezwoda WRA, Gear J. Platelet parameters: Part II. Platelet volume-number relationships in various normal and disease states. SAMJ 1988; 73(9): 39–43.
• ) Akkuş İ, Şekeroğlu R, Üzer A. Selenyum: dağılışı metabolizması ve fizyopatolojisi. S.Ü.Tıp Fak Derg 1991; 7(4): 547–51.
• ) Lansiter JW, Hardy M, Edwards J. Selenium Animal Nutrition. Restong Publishing Company, ; 202–4. ) Karakılçık AZ, Aksakal M, Özguner F, Çay M, Naziroglu M. Maternal and foetal selenium concentrations and their interrelationships in akkaraman sheep. Indian J Anim Sci 1997; 67 (1): –22.
• ) Dhur A, Galon P, Hercberg S. Relationship between selenium and resistance against infection. Comp Biochem Physiol 1990; 96 (2): 271–80.
• ) Kiremidjian-Schumacher L, Stotzky G. Selenium and immune responses. Environ Res. ; 42(2): 277-303. ) Çadırcı MŞ, Dörtbudak MY, Karakılçık AZ. Deneysel Diyabette Glukoz, Lipit Değerleri, Trombosit İndeksleri ve Böbrek Dokusu Üzerine E Vitamini ve Selenyumun Etkisi. FÜ Sağ Bil Tıp Derg, 2013; 27 (1): 13–8.
• ) Kim SW, Ryu GH, Lee I, Koh JJ, Min BG, Lee HK. Adhered platelet morphology in diabetes mellitus. Diabetes Metab 1995; 21(1): 50–3.