Büyüme faktörlerinin kordon kanı kök hücre içeriği üzerine etkisinin hücrelerde Rodamin123 birikimi ile belirlenmesi

Yıl 2007, Cilt: 4 Sayı: 2, 45 - 49, 01.08.2007

Faruk Suzergoz Ali Osman Gürol Serap Erdem

Öz

Amaç: Mitokondri zarında toplanan rodamin123 Rd123 , hücrede mitokondri sayısı veya aktivitesi hakkında bilgi vermekte ve kök hücreyi fonksiyonel açıdan tanımlamada bir belirteç olarak kullanılmaktadır. Kök hücreler metabolik olarak inaktif veya uyku fazında olduklarından, bu hücrelerde Rd123 birikimi oldukça düşük düzeydedir. Çalışmamızda, büyüme faktörlerinin kordon kanı KK kök hücre içeriği üzerine etkisini araştırmak için, in vitro kültür periyodunda hücrelerin Rd123 alımını belirlemeyi amaçladık. Gereç Ve Yöntem: Sağlıklı ve zamanında doğum yapan 15 gönüllüden KK örnekleri alındı. Mononükleer hücreler ayrıldıktan sonra interlökin-3 IL-3 , IL-6, IL-11, stem cell faktör SCF , flt3/flk2 ligand FL ve trombopoetin TPO ile 14 gün inkübe edildi. Kültür öncesi, kültürün 7. ve 14. günlerinde hücreler alınarak Rd123 alım düzeyleri flow sitometride analiz edildi. Sonuçlar: Kök hücre üzerine etkili büyüme faktörleri ile yapılan kısa süreli kültürün 7. gününde, hücrelerin Rd123 alımında istatistiksel olarak anlamlı düşüş gözlendi p

Anahtar Kelimeler

Kordon kanı, kök hücre, Rodamin123, büyüme faktörleri

Determining Effects Of The Growth Factors On Stem Cell Content Of Cord Blood By Rhodamine123 Upthake Of The Cells

Öz

Determining Effects Of The Growth Factors On Stem Cell Content Of Cord Blood By Rhodamine123 Upthake Of The Cells Objectıve: Rhodamine 123 Rh123 that accumulate in mitocondrial membran is given information about mitochondrium counts and their activities and it is used for identify for stem cells the methabolic aspects. Due to the stem cells are inactive or dormant, Rh123 accumulation is very low in these cells. In this study, we aimed to investigate the effects of growth factors on stem cell content of cord blood CB by determinig Rh123 uptake by these cells during in vitro culture period. Materıals Ve Methods: CB samples obtained from 15 healthy volunteers delivering in term. After separation, mononuclear cells incubated for 14 day presence of interleukin-3 IL-3 , IL-6, IL-11 stem cell faktör SCF , flt3/flk2 ligand FL ve trombopoetin TPO . Cells from before, day 7 and day 14 of cultures were analysed for Rh123 uptake by flow cytometry. Results: At day 7 of short term cultures initiated with growth factors known to be effective on stem cells, statistically significant p

Anahtar Kelimeler

Cord blood, stem cell, Rhodamin123, growth factors

Kaynakça

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