Hemostatic Efficacy of Algan Hemostatic Agent in Renal Vein Incision Model in Rats

Yıl 2023, Cilt: 20 Sayı: 1, 13 - 18, 27.04.2023

Dila Şener , Zeynep Kerime Şanlı , Abdulaziz Abouhosa , Deniz Mukaddes Türet , Uğur Şeker

https://doi.org/10.35440/hutfd.1187636

Öz

Background: The aim of this study is to evaluate the acute hemostatic effects of liquid and powder forms of AHA in severe bleeding model of rat renal vein.
Materials and Methods: 10-12 weeks old, 24 male Wistar albino rats were randomly divided into three groups of eight animals each as the control, AHA liquid, AHA powder. The left renal artery and vein were revealed with a 3 cm vertical incision and the tip of the injector was used to puncture renal vein, causing bleeding. In all animals, regular gauze patch was pressed over the incised area for 10 seconds as soon as the bleeding began. Immediately after removing the sponge; physiological saline, AHA liquid and powder were applied with compress to injured site of relevant groups for 2 minutes, respectively. The time was recorded by chronometer and the area was checked after two minutes. Hemostasis that was not achieved after three applications was accepted as failure.
Results: Cessation of the bleeding failed in all rats after three successive 2-minute physiological saline impregnated gauze applications in control group. Among AHA-treated groups, significant hemostasis efficacy was obtained from the liquid form than that of powder. Within first two minutes, bleeding in renal veins of five rats was controlled successfully in liquid group whereas powder group achieved hemostatic success in four rats.
Conclusions: The vessel incision model in our study revealed fast-acting physical hemostatic properties of the liquid and powder formulations of AHA in the renal vein. This natural applicable product could be used to induce hemostasis in rat models of bleeding caused by various renal damages or trauma, and it could also prevent local bleeding in humans. More studies are needed to compare the efficacy of different formulations of the polysaccharide-based herbal product in various organs and surgical models.

Anahtar Kelimeler

Hemostasis, Kidney, Renal Vein, Algan Hemostatic Agent, Bleeding, Rat

Destekleyen Kurum

Yok

Proje Numarası

Yok

Teşekkür

We thank to the Algan Group Health Services (Algan Group Health Services Import and Export Industry and Trade Limited Company, Istanbul, Turkey) for providing AHA forms.

Algan Hemostatik Ajan’ın Sıçan Renal Ven İnsizyon Modelinde Hemostatik Etkinliği

Öz

Amaç: Bu çalışmanın amacı, sıçan renal veninin şiddetli kanama modelinde sıvı ve toz AHA formlarının akut hemostatik etkilerini değerlendirmektir.
Materyal ve metod: 10-12 haftalık, 24 erkek Wistar albino sıçan kontrol, AHA sıvı ve AHA toz olarak her biri sekiz hayvan içeren üç gruba rastgele ayrıldı. Sol renal arter ve ven 3 cm'lik vertikal insizyon ile ortaya çıkarıldı. Renal venin kesi yaralanması için enjektör ucu kullanıldı ve kanamaya neden oldu. Kanama başladığında tüm hayvanlarda insize edilen alan üzerine 10 saniye süresince gazlı bez ile bası uygulandı. Ardından, serum fizyolojik, AHA sıvı ve toz ilgili gruplarda aynı kesi bölgesine sırasıyla iki dakika süreyle uygulandı. Süre kronometre ile kaydedildi ve kanama alanı iki dakika sonar control edildi. Üç uygulama sonrasında hemostaz sağlanamadıysa, başarısız olarak kabul edildi.
Bulgular: Kontrol grubundaki tüm sıçanlarda ardışık 2 dakikalık serum fizyolojik emdirilmiş gazlı bez uygulamalarında kanama durdurulması başarısız oldu. AHA uygulanan gruplarda, sıvı formdan toza göre daha önemli hemostaz etkinliği sağlandı. İlk iki dakika içinde, sıvı grubunda beş sıçanın böbrek damarlarındaki kanama kontrol edilirken, toz grubunda dört sıçanda hemostatik başarı elde edildi.
Sonuç: Çalışmamızdaki damar kesi modeli, renal vende AHA' nın sıvı ve toz formülasyonlarının hızlı etkili fiziksel hemostatik özelliklerini ortaya çıkarmıştır. Bu doğal uygulanabilir ürün, çeşitli böbrek hasarları ya da travmaya bağlı sıçan kanama modellerinde hemostaz sağlamak için kullanılabilir ve ayrıca insanlarda lokal kanamayı önleyebilir. Polisakkarit bazlı bitkisel ürünün farklı formülasyonlarının çeşitli organlarda ve cerrahi modellerde etkinliğini karşılaştırmak için daha fazla çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

Hemostaz, Böbrek, Renal Ven, Algan Hemostatik Ajan, Kanama, Sıçan

Proje Numarası

Yok

Kaynakça

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