The Most Common Molecular Diagnostic Method in Microbiology: Polymerase Chain Reaction

Yıl 2023, , 118 - 125, 22.06.2023

Oğuz Kağan Türedi , Esra Şeker

https://doi.org/10.31196/huvfd.1246738

Cited By: 1

Öz

This review briefly explains Polymerase Chain Reaction (PCR) types commonly used in diagnostic microbiology. PCR, first discovered by Kary Banks Mullis in 1985 for DNA search, is an in vitro method involving the enzymatic amplification of a specific DNA segment by primers, creating many copies. Today, interest in PCR and PCR-based diagnostic methods is increasing, and PCR remains up-to-date as the most widely used molecular diagnostic method in the diagnosis of infectious diseases of humans and animals and epidemiological studies. When molecular diagnostic procedures are constantly updated, new techniques are added, and become more affordable, accessible, and applicable in routine diagnosis, the frequency and equality of use of these techniques in diagnostic microbiology will increase at the same rate.

Anahtar Kelimeler

Diagnostic microbiology, molecular diagnosis, PCR, PCR types

Mikrobiyolojide En Yaygın Moleküler Tanı Yöntemi: Polimeraz Zincir Reaksiyonu

Öz

Sunulan bu derlemenin amacı diagnostik mikrobiyoloji alanında yaygın olarak kullanılan Polimeraz Zincir Reaksiyonu (PZR) tipleri hakkında kısa bilgi verilmesidir. İlk olarak 1985 yılında Kary Banks Mullis tarafından DNA araştırması için keşfedilen PZR, belirli bir DNA segmentinin primerler aracılığıyla enzimatik amplifikasyonunu sağlayarak çok sayıda kopyasını oluşturan in vitro bir yöntemdir. Günümüzde PZR ve PZR tabanlı tanı yöntemlerine ilgi giderek artmakta ve PZR, insan ve hayvanların infeksiyöz hastalıklarının tanısında ve epidemiyolojik araştırmalarda en yaygın kullanılan moleküler tanı yöntemi olarak güncelliğini korumaktadır. Sürekli güncellenen ve yeni teknikler eklenen moleküler tanı yöntemleri rutin tanıda daha ekonomik, ulaşılabilir ve uygulanabilir hale geldiğinde tanısal mikrobiyoloji alanında bu tekniklerin kullanım sıklığı ve çeşitliliği aynı oranda artacaktır.

Anahtar Kelimeler

Diagnostik mikrobiyoloji, moleküler tanı, PZR, PZR tipleri

Kaynakça

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