Aydın ve İzmir İllerindeki Koyun ve Keçilerde Pestivirus Enfeksiyonunun Serolojik ve Virolojik Olarak Araştırılması

Abstract

Bu çalışma ile Aydın ve İzmir illerindeki koyun ve keçilerde pestivirus enfeksiyonunun serolojik ve virolojik olarak araştırılması amaçlanmıştır. Çalışmada 128 adet keçi, 332 adet koyun olmak üzere toplam 460 adet hayvandan serum örnekleri elde edildi. Alınan kan serumu örneklerinde, bovine viral diarrhea virusunun (BVDV) referans suşu NADL kullanılarak virus nötralizasyon testi ile antikor varlığı araştırıldı. Toplam 348 adet (116 adet keçi, 232 adet koyun) hayvandan alınan tam kan örnekleri pestivirus spesifik antijen varlığı yönünden ticari Enzyme Linked Immunosorbent Assay (ELISA) (Serelisa^® BVD p80Ag Mono Indirect, Synbiotics, Europe, Lyon, France) ile test edildi. Keçi örneklerinin %41.40’ının (53 /128), koyun örneklerinin ise %47.59’unun (158/332) seropozitif olduğu saptandı. Seropozitif bulunan toplam 211 serum örneğinin %37.44’ünde 1/5, %34.12’sinde 1/10, %14.21’inde 1/20, %5.21’inde 1/40, %5.68’inde 1/80 ve %3.31’inde ise 1/160 olarak SN₅₀ değerleri belirlendi. Test edilen tam kan örneklerinin antijen yönünden negatif olduğu belirlendi. Bu çalışmada, Aydın ve İzmir illerinde örneklenen koyun ve keçilerde viremik hayvan tespit edilmemiş olmasına rağmen, %45.87 (211/460) oranında seropozitiflik saptanması pestivirusların bölgede sirküle olduğunu ve önemli ekonomik kayıplara neden olabileceğini göstermektedir.

Keywords

• Antijen,Antikor,Keçi,Koyun,Pestivirus

A Serological and Virological Investigation of the Pestivirus Infections in Sheep and Goats in the Aydin and Izmir Provinces

Abstract

The objective of this study was serological and virological investigation of pestivirus infections in sheep and goats in the Aydin and Izmir provinces. In the study, 460 serum samples were collected from 128 goats and 332 sheep. The presence of antibodies in the blood serum samples was investigated using NADL, the reference strain for bovine viral diarrhea virus (BVDV) in virus neutralization test. In total, 348 whole blood samples collected from 116 goats and 232 sheep were tested for the presence of the pestivirus specific antigens using commercially available enzyme linked immunosorbent assay (ELISA) (Serelisa® BVD p80ag Mono Indirect, Synbiotics, Europe, Lyon, France). 41.40% (53/128) of the goat and 47.59% (158/332) of the sheep samples were found to be seropositive. SN₅₀ values were 1/5 in 37.44%, 1/10 in 34.12%, 1/20 in 14.21%, 1/40 in 5.21%, 1/80 in 5.68% and 1/160 in 3.31% of the 221 seropositive serum samples. The whole blood samples tested were negative for the antigen. Although no viremia was detected in any of the sheep and goat tested in this study, the 45.87% (211/460) rate of seropositivity shows that Pestiviruses may be circulating in the region and may cause significant economic losses.

Keywords

• Pestivirus,Antigen,Antibody,Goat,Sheep.

References

1. Albayrak H, Özan E, 2012: The investigation of Pestivirus and Rift Valley Fever Virus infections in aborted ruminant foetuses in the Blacksea Region in Turkey. Kafkas Univ Vet Fak Derg, 18, 3, 457-461.
2. Ataseven VS, Ataseven L, Tan T, Babur C, Oğuzoğlu TÇ, 2006: Seropositivity of agents causing abortion in local goat breeds in Eastern and Southeastern Anatolia, Turkey. Rev Med Vet, 157, 955-961.
3. Avcı O, 2010: Konya ve çevresinde abort problemli koyun sürülerinde border disease virus enfeksiyonun araştırılması. Doktora tezi. Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü Konya.
4. Azkur AK, Gazyağcı S, Aslan ME, Ünal N, 2011: Molecular and serological characterization of pestivirus infection among sheep in Kirikkale Turkey. Kafkas Univ Vet Fak Derg, 17(Suppl A), 83-92.
5. Burgu İ, Öztürk F, Akça Y, 1984: Tahirova devlet üretme çiftliği koyunlarında viralenfeksiyonlar üzerinde serolojik araştırmalar. Ankara Üniv Vet Fak Derg, 31, 2, 167-179.
6. Burgu İ, Öztürk F, Akça Y, Toker A, Frey HR, Liess B, 1987: Investigations on the occurrence and impact of bovine viral diarrhea (BVD) virus infections in sheep in Turkey. Dtsch Tierarztl Wochenschr, 94, 292-294.
7. Burgu İ, Özkul A, 1993: Detection by cultural isolation of bovine virus diarrhea (BVD) virus following field infections in cattle and their fetuses. Dtsch Tierarztl Wochenschr, 100, 361-363.
8. Burgu İ, Akça Y, Alkan F, Özkul A, Karaoğlu TM, Bilge Dağalp S, Oğuzoğlu TÇ, Yeşilbağ K, 2001: Koyunlarda doğum öncesi ve sonrası dönemlerde bovine viral diarrhea (BVD) virus enfeksiyonunun serolojik virolojik ve patogenez yönünden araştırılması. Turk J Vet Anim Sci, 25, 551-557.

9. Carlsson U, 1991: Border Disease in sheep caused by transmission of virus from cattle persistently infected with bovine virus diarrhea virus. Vet Rec, 128, 141-147.
10. Carlsson U, Belak K, 1994: Border disease virus transmitted to sheep and cattle by a persistently infected ewe: epidemiology and control. Acta Vet Scand, 35, 1, 79-88.
11. Gazyağcı S, Azkur AK ve Çağlayan O, 2011: Comparison of hematological and biochemical parameters in sheep naturally and pesistently infected with a border disease virus. Trop Anim Health Prod, 43, 553-556.
12. Gür S, 2009: An investigation of border disease virus in sheep in Western Turkey. Trop Anim Health Prod, 41, 1409-1412.
13. Gür S, Erol N, Yapıcı O, 2009: Afyon, Konya ve Eskişehir illerinde keçilerde pestivirus ve parainfluenzavirus tip 3 enfeksiyonlarının serolojik olarak araştırılması. Kocatepe Vet J, 2, 1, 23-27.
14. Hasırcıoğlu S, Kale M, Acar A, 2009: Investigation of pestivirus in aborted sheep and goats in Burdur region. Kafkas Univ Vet Fak Derg, 15, 2, 163-167.
15. Lindenbach B, Thiel HJ, Rice CM, 2007: Family Flaviviridae, 1101-1133. In: Knipe DM, Howley PM (Eds.), Fields Virology, 5th Edition. Lippincott-Raven Publishers, Philadelphia.
16. İssi M, Gülaçtı İ, Kızıl Ö,Karapınar T, Bulut H, Gül Y, 2006: Kliniğimizde gözlemlenen reverze transkriptaz–polimeraz zincir reaksiyonu (RT-PCR) ile doğrulanan mukoza hastalığı olguları. Fırat Univ Sağlık Bilim Derg, 20, 253-258.
17. İssi M, Gül Y, Gürçay M, Gök T, 2012: Elazığ yöresindeki koyunlarda saptanan pestivirus enfeksiyonu. Fırat Univ Sağlık Bilim Derg, 26, 3, 165-169.
18. Juliá S, Craig MI, Jiménez LS, Pinto, GB ve Weber EL, 2009: First report of BVDV circulation in sheep in Argentina. Prev Vet Med, 90, 274-277.
19. Krametter-Froetscher R, Duenser M, Preyler B,Theiner A, Benetka V, Moestl K, Baumgartner W, 2010: Pestivirus infection in sheep and goats in West Austria. Vet J, 186, 342-346.
20. Lamontagne L, Roy R, 1984: Presence of antibodies to bovine viral diarrhoea-mucosaldiseasebirus (Borderdisease) in sheep and goat flocks in Quebec. Can J Comp Med, 48, 225-227.
21. Löken T, Krosgrud J, Larsen IL, 1991: Pestivirus infections in Norway. Serological investigation in cattle, sheep and pigs. Acta Vet Scand, 32, 27-34. Mishra N, Pitale SS, Rajukumar K, 2012: Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India. Acta Virol, 56, 209-215.
22. O’Neill RG, O’Connor M ve O’Reilly PJ, 2004: A survey of antibodies to pestivirus in sheep in the Republic of Ireland. Ir Vet J, 57, 525-530.
23. Oğuzoğlu TÇ, Tan MT, Toplu N, Demir AB, Bilge-Dagalp S, Karaoglu T, Ozkul A, Alkan F, Burgu I, Haas L, Greiser-Wilke I, 2009: Border disease virus (BDV) infections of small ruminants in Turkey: A new BDV subgroup. Vet Microbiol, 135, 374-379.
24. Okur-Gümüşova S, Yazıcı Z, Albayrak H, 2006: Pestivirus seroprevalence in sheep populations from inland and coastal zones of Turkey. Rev Med Vet, 157, 595-598.
25. Ozan E, Turan HM, Albayrak H, Cavunt A, 2012: Serological determination of pestivirus, bluetongue virus and peste des petits ruminants virus in small ruminants in Samsun province of Turkey. Atatürk Üniv Vet Bilim Derg, 7, 1, 27-33.
26. Passler T, Riddell KP, Edmondson MA, Chamorro MF, Neill JD, Brodersen BW, Walz HL, Galik PK, Zhang Y and Paul H Walz, 2014: Experimental infection of pregnant goats with bovine viral diarrhea virus (BVDV) 1 or 2. Vet Res, 45, 38, 1-10.
27. Radostits OM, Gay CC, Hinchcliff KW, Constable PD, 2008: Veterinary Medicine. 10th edition, Edinburgh, London, New York, Oxford, Philadelphia, St Louis, Sydney, Toronto: Saunders Elsevier.
28. Sawyer MM, Schore CE, Osburn BI, 1991: Border Disease of sheep- Aspects for diagnostic and epidemiologic consideration. Arch Virol, Supplementum, 3, 97-100.
29. Schaller P, Vogt HR, Strasser M, Nettleton PF, Peterhans E, Zanoni R, 2000: Seroprevalence of maedi-visna and border disease in Switzerland. Schweiz Arch Tierheilkd, 142, 145-153.
30. Steel RGD, Torrie JH, 1980: Principles and Procedures of Statistics: A Biometrical Approach, 2nd Ed. New York.
31. Tan MT, Karaoğlu MT, Erol N, Yıldırım Y, 2006: Serological and virological investigations of bovine viral diarrhoea virus (BVDV) infection in dairy cattle herds in Aydın province. Turk J Vet An Sci, 30, 299-304.
32. Ural K, Ulutaş B, Ulutaş PA, Gültekin M, Atasoy A, 2011: A flock level analysis of an outbreak of natural border disease in sakiz ewes and their progeny. Acta Sci Vet, 39, 3, 977.
33. Van Oirschot JT, 1983: Congenital infections with non-arbo togaviruses. Vet Microbiol, 8, 321- 361.
34. Yazıcı Z, Serdar MS, Gümüşova SO ve Albayrak H, 2012: Molecular diagnosis and seroepidemiology of pestiviruses in sheep. Vet Arhiv, 82, 35-45.