In Vitro Sitotoksisite Testleri

Yıl 2017, , 112 - 118, 30.06.2017

Orhan Tokur Abdurrahman Aksoy

https://doi.org/10.31196/huvfd.325794

Cited By: 16

Öz

Kimyasal ve biyolojik maddeler ya da fiziksel
etkenler, hücreleri değişik derecelerde etkileyerek sitotoksisiteye yol
açabilirler. Bir maddenin biyolojik davranışının anlaşılabilmesi için hücreler
üzerindeki toksik ya da non-toksik etkisinin belirlenmesi gereklidir. İn vitro
sitotoksisite testleri, ilaç niteliği taşıyan veya toksik profili araştırılan
maddelerin değerlendirilmesi amacıyla hücre kültüründe gerçekleştirilen ölçüm
metodlarıdır. Bu testlerle çok sayıda maddenin kısa sürede analiz edilmesi mümkün
olmakta ve yapılacak hayvan denemeleri için temel veriler elde edilebilmektedir.
Farklı mekanizmalara ve hassasiyetlere sahip çok sayıda sitotoksisite testi
bulunmakla birlikte bu derlemede, araştırmacılar tarafından sıkça tercih edilen
tetrazolyum testleri (MTT, MTS, XTT, WST), LDH testi, alamalar mavisi testi ve
biyolüminesans testleri ele alınmıştır.

Anahtar Kelimeler

In vitro, Sitotoksisite, Hücre canlılık testleri

In Vitro Cytotoxicity Assays

Öz

Chemical, biological and physical factors may cause
cytotoxicity by affecting cells at different degrees. To understand the
biological characteristic of a compound, it is essential to determine its toxic
or non-toxic effects. In vitro cytotoxicity assays (cell viability assays) are
cell culture based measuring methods that usually used for either evaluating possible
drug candidates or investigating cytotoxic profiles of some compounds. It is
possible to assess many compounds in a short period of time and obtain
fundamental information necessary for further animal experiments through this
methods. There are many cell viability assay methods exist based on
colorimetric, enzymatic, luminometric principals etc that have different mechanisms
and varied sensitivity. The popular assays, such as MTT, MTS, XTT, WST, LDH
enzyme assay, alamar blue assay, ATP bioluminescent assay have summarized in
this rewiev. 

Anahtar Kelimeler

In vitro, Cytotoxicity, Cell viability assays

Kaynakça

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