Determination of Borrelia burgdorferi Seropositivty in Arabian Thoroughbred Horses in Sanliurfa

Abstract

Lyme Borreliosis or Lyme disease is a zoonotic disease caused by Borrelia burgdorferi. The transport of the disease is by the Ixodesticks. The disease affects various organs and systems of humans and domesticated animals (horses, cattle, dogs, cats, sheep, etc.). Veterinarians generally misdiagnose the disease because Lymeborreliosis imitates many diseases clinically. Due to difficulties in culturing of the agent, serological methods such as ELISA and IFAT are widely used for diagnosis of the disease. In this study, it was aimed to determine the seropositivity of Lyme Borreliosis in healthy thoroughbred Arabian horses in Sanliurfa. For this aim, between Autumn 2016 and Spring 2017, 186 serum samples were taken from randomly chosen pedigree purebred Arabian horses owned by the local people. Anti-Borrelia burgdorferi IgG antibodies were investigated by a commercial ELISA kit. Of 186 serum samples examined, 11 (5.9%) were found as positive, and three (1.6%) serum samples were found as doubtful. The rest of the serum samples (172, 92.5%) were found to be negative. This study is the first reporting the presence of anti-B. burgdorferi antibodies in Sanliurfa province. Due to both economic losses caused by the agent and zoonosis character of the disease, veterinarians should not ignore Lyme Borreliosis in their clinical studies. In additon, necessary preventive measures should be taken against the disease, and further studies should be conducted to search the current status of the disease in different animal species in the region

Keywords

• Lyme Borreliosis,Horse,ELISA,Seroprevalence

Şanlıurfa’da Safkan Arap Atlarında Borrelia burgdorferi Seropozitifliğinin Belirlenmesi

Abstract

Lyme Borreliozis veya Lyme hastalığı, Borrelia burgdorferi’nin neden olduğu kenelerle nakladilen zoonotik bir infeksiyondur. Hastalığın taşınması, Ixodes cinsi kenelerle olmaktadır. Lyme Borreliozis evcil hayvanların (at, sığır, köpek, kedi, koyun vb.) ve insanların çeşitli organ ve sistemlerini etkilemektedir. Hastalık, klinik olarak pek çok hastalığı taklit ettiğinden veteriner hekimler tarafından genellikle gözden kaçırılmaktadır. Etkenin kültüre edilmesi zor olduğundan teşhiste genellikle ELISA ve IFAT gibi serolojik yöntemler kullanılmaktadır. Bu çalışmada, Lyme Borreliozis’in Şanlıurfa’daki sağlıklı Safkan Arap atlarında seropozitiflik durumunun belirlenmesi amaçlanmıştır. Bu amaca yönelik olarak Eylül 2016- Temmuz 2017 tarihleri arasında halk elinde yetiştiriciliği yapılan pedigrili damızlık Safkan Arap atlarından tesadüfi örnekleme ile 186 kan serum örneği alındı. Serum örneklerinde anti-B. burgdorferi IgG antikorları ticari bir ELISA kiti ile araştırıldı. İncelenen 186 serum örneğinin 11'i (%5.9) pozitif, 3'ü (%1.6) şüpheli ve 172'si de (%92.5) negatif bulundu. Bu çalışma ile ilk kez Şanlıurfa İli'nde Safkan Arap atlarında anti-B. burgdorferi antikorlarının varlığı gösterilmiştir. Hem etkenin neden olduğu ekonomik kayıplar hem de infeksiyonun zoonoz karakteri nedeniyle, veteriner hekimler klinik çalışmalarında Lyme Borreliosis'i de dikkate almalıdırlar. Ayrıca, hastalığa karşı gerekli önleyici tedbirler alınmalı ve bölgedeki farklı hayvan türlerinde hastalığın mevcut durumunu araştırmak için daha ileri çalışmalar yapılmalıdır.

Keywords

• Lyme,At,ELISA,Seroprevalans

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