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Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi

Yıl 2017, Cilt: 6 Sayı: 1, 11 - 18, 30.06.2017
https://doi.org/10.31196/huvfd.325672

Öz

Ratlarda parasetamol ile
oluşturulan hepatotoksisite üzerine Taraxacum
officinale
etanol ekstraktının kan ve karaciğer dokusundaki bazı
biyokimyasal parametrelere ve karaciğer dokusunun histopatolojisi üzerine etkileri
araştırıldı. Bu çalışmada, 5 aylık yaşta 36 adet Sprague Dawley rat kullanıldı.
Sprague Dawley ratlar rastgele her grupta 6 rat olacak şekilde 6 gruba ayrıldı.
Parasetamol (PARA) verilecek gruplardaki hayvanlar 24 saat aç bırakılıp
ekstrakt verildikten 1 saat sonra 2 g/kg oral olarak (p.o.) PARA verildi.
1.Grup (Kontrol Grubu), %5’lik DMSO intraperitoneal (i.p.), 2.Grup (TOE1
Grubu), 200 mg/kg/gün/i.p. Taraxacum
officinale
ekstraktı (TOE) %5’lik DMSO’da çözülerek i.p, 3.Grup (TOE2
Grubu), 250 mg/kg/gün/i.p. Taraxacum
officinale
ekstraktı, 4.Grup (PARA Grubu), 2 g/kg/p.o. Parasetamol, 5.Grup
(PTOE1 Grubu), Parasetamol 2 g/kg/gün/p.o. + TOE 200 mg/kg/gün/i.p, 6.Grup
(PTOE2 Grubu), Parasetamol 2 g/kg/gün/p.o. + TOE 250 mg/kg/gün/i.p 8 gün
boyunca uygulandı. Çalışma sonunda ratlardan alınan kan ve karaciğer dokusundan
biyokimyasal ve histopatolojik analizler yapıldı. Parasetamol grubunda plazma AST, ALT, ALP, MDA, CAT ve nitrit
düzeyleri; karaciğer MDA, nitrit ve nitrat düzeyleri kontrol grubuna göre
artarken (P<0.001); plazma GSH (P<0.001), SOD (P<0.001) ve GPx
(P<0.05) düzeyleri; karaciğer GSH, CAT ve SOD (P<0.001) düzeyleri anlamlı
olarak azaldı. Parasetamolün ratlarda oluşturduğu hepatotoksisiteyi Taraxacum officinale bitkisinin toprak
üstü aksamlarından yapılan etanol ekstraktının 200 mg/kg uygulanan miktarının
hepatotoksisiteyi anlamlı olarak azalttığı ve lipid peroksidasyonu önlediği,
TOE 250 mg/kg uygulanan hayvanlarda toksik etki yaptığı tespit edildi. Elde
edilen ekstraktın hepatotoksisite üzerine ve plazma ve karaciğer biyokimyasal
parametreleri üzerine olumlu etkilerinin olduğu saptansa bile karaciğerde
oluşan hasarı düzeltmek için zamana gereksinim duyulduğu kanaatine varıldı.

Kaynakça

  • Adam B, 2012: Yiğitoğlu R. Tıbbi Biyokimya, 1. Baskı. İstanbul, Nobel Tıp Kitabevi, 189-249.
  • Aktaş Ö, Eskiocak S, Özgün GS, Yalçın Ö, Süt N, 2013: Asetaminofen ile toksik hepatit oluşturulan ratlarda L-karnitinin etkisi. Turkish Journal of Biochemistry/Türk Biyokimya Dergisi, 38, 475-482.
  • Al-Malki Abdulrahman L, Abo-Golayel MK, 2013: Hepatoprotective Efficacy of Chicory alone or combined with Dandelion leaves against induced liver damage. Life Science Journal, 10(4), 140-157.
  • Albayrak A, 2013: Serotonin-7 reseptörlerinin parasetamol ile indüklenen deneysel karaciğer toksisistesindeki yeri ve önemi. Türk Farmakoloji Derneği Farmakoloji Eğitiminde Kuşaklararası Bilimsel Etkileşme Seminerleri Programı, Bursa.
  • Anonim, 2013a: http://www.naturalfoodbenefits.com /display.asp?CAT=2&I=120. Natural food benefits, Erişim tarihi; 20.09.2013.
  • Anonim,2013b:http://www.naturallifemagazine.com/1204/dandelions.htm. Natural life magazine, Erişim tarihi; 20.09.2013.
  • Ball CR, 1996: Estimation and identification of thiols in rat spleen after cysteine or glutathione treatment pelevance to protection against nitrojen mustards. Biochemical Pharmacology, 15, 809-816.
  • Champe PC, Harvey RA, Ferrier D, 2007: Lippincott’s Illustrated Reviews: Biochemistry. Çeviri: Ulukaya E. Biyokimya, 3. Baskı, İstanbul, Nobel Tıp Kitabevi.
  • Chance B, Greenstein DS, 1992: The mechanism of catalase actions-steady state analysis. Archives of Biochemistry and Biophysics, 37, 301-339.
  • Chattopadhyay RR, 2003: Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: part II. Journal of Ethnopharmacol, 89, 217-219.
  • Cho SY, Oh YJ, Park J, 2003: Effect of dandelion (Taraxacum officinale) leaf extracts on hepatic antioxidative system in rats fed high cholesterol diet. Journal of the Korean Society of Food Science and Nutrition, 2, 458-463.
  • Colle D, Arantes LP, Gubert P, Luz SCA, Athayde ML, Rocha JBT, Soares FAA, 2012: Antioxidant properties of Taraxacum officinale leaf extract are involved in the protective effect against hepatoxicity induced by acetaminophen in mice. Journal of Medicinal Food, 15, 549-556.
  • Davis PH, 1965: Flora of Turkey and The East Aegea Islands, Edinburg, The University Press.
  • Domitrović R, Jakovac H, Romić Ž, Rahelić D, Tadić Ž, 2010: Antifibrotic activity of Taraxacum officinale root in carbon tetrachloride-induced liver damage in mice. Journal of Ethnopharmacology, 130, 569-577.
  • Fernandez V, Videla LA, 1981: Effect of acute and chronic ethanol ingestion on the content of reduced glutathione of varios tissues of the rat. Experientia, 37, 392-394.
  • Fortea MI, Lopez-Miranda S, Serrano-Martinez A, Carreno BJ, Nunez-Delicado E, 2009: Kinetic characterisation and thermal ınactivation study of polyphenol oxidase and peroxidase from table grape (Crimson Seedless). Food Chemistry, 113, 1008-1014.
  • Gallaher RN, Gallaher K, Marshall AJ, 2006: Mineral analysis of ten types of commercially available tea. Journal of Food Composition and Analysis, 19, 53-57.
  • Gonzalez-Castejon M Visioli F, Rodriguez-Casado A, 2012: Diverse biological activities of dandelion. Nutrition Reviews, 70, 534-547.
  • Goth L, 1991: A simple method for determenation of serum catalase activity and revision of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196, 143-152.
  • Hoşbaş S, Hartevioğlu A, Pekcan M, Deliorman Orhan D, 2011: Assessment of hepatoprotective activity of Achillea biebersteinii ethanol extract on carbon tetrachloride-induced liver damage in rats. FABAD Journal of Pharmaceutical Sciences, 36, 33-39.
  • Hu C, Kitts DD, 2004: Luteolin and luteolin-7-O-glucoside from dandelion flower suppress iNOS and COX-2 in RAW264.7 cells. Molecular and Cellular Biochemistry, 265, 107-113. Jaeschke H, Bajt LM, 2006: Intracellular signaling mechanisms of acethominophen-induced liver cell death. Toxicological Sciences, 89, 31-41.
  • Jung K, Henke W, 1996: Developmental changes of antioxidant enzymes activity in kidney and liver from rats. Free Radical Biology and Medicine, 20, 613-617.
  • Kayaalp O, 2005: Analjezikler: Tıbbi Farmakoloji. İçinde: Lippincott: Analgesic Application, 7.Baskı, Ankara, 223-245.
  • Kim MY, Cheong SH, Kim MH, Son C, Yook HS, Sok DE, Cho JHK, ChunH, Kim MR, 2009: Leafy vegetable mix supplementation improves lipid profiles and antioxidant status in C57BL/6J mice fed a high fat and high cholesterol diet. Journal of Medicinal Food, 12, 877-884.
  • Koç H, 2002: Doğrudan, doğadan bitkilerle sağlıklı yaşama. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölüm Yayını, Tokat, Ümit Ofset.
  • Kordali Ş, Çakır A, Akcin TA, Mete E, Akcin A, Aydın T, Kılıç H, 2009: Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Industrial Crops and Products, 29, 562-570.
  • Krishna A, Chaitanya D, Siva RC and Manohar RA, 2012: Hepatoprotective effect of biherbal ethanolic extract against paracetamol-induced hepatic damage in albino rats. Journal of Ayurveda and Integrative Medicine, 198.
  • Kuntz E, Kuntz HD, 2008: Hepatology Textbook and Atlas, 3rd ed. Germany, Springer Press.
  • Matkovics B, Szabo L, Varga IS, 1988: Determination of enzyme activities in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika, 15, 248-249.
  • Nahida T, Qazi MA, Shah A, 2011: Curative activity of ethanol extract of Taraxacum officinale weber. against CCl4 induced hepatocellular damage in albino rats. Journal of Pharmacy Research, 4,3, 687-689.
  • Parmar SR, Dave GS, Patel HV, Kıran K, 2009: Hepato-Protectıve value of some plants extract against carbon tetrachlorıde toxicity in male rats. Journal of Cell and Tissue Research, 9, 1737-1743.
  • Parmar SR, Vashrambhai PH, Kalia K, 2010: Hepatoprotective activity of some plants extract against paracetamol induced hepatotoxicity in rats. Journal of Herbal Medicine and Toxicology, 4, 101-106.
  • Pauli-Magnus C, Stieger B, Meier Y. Kullak-Ublick GA, Meier PJ, 2005: Enterohepatic transport of bile salts and genetics of cholestasis. Journal of Hepatology, 43, 342-357.
  • Placer ZA, Cushman LL, Johnson BC, 1966: Esimaion of product of lipid peroxidaion (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry, 16, 359-364.
  • Plants A and Karaj I. The effects of Taraxacum officinale L. and Berberis vulgaris L. root extracts on carbon tetrachloride induced liver toxicity in rats. Journal of Medicinal Plants, 2010.
  • Raja B, Mol SD, 2010: The protective role of vanillic acid against acetaminophen induced hepatotoxicity in rats. Journal of Pharmacy Research, 1480.
  • Rang HP, Dale MM, Ritter JM, Flower RJ, Henderson G, 2011: Rang&Dale’s Pharmacology. 7th ed. Elsevier Churchill Livingston, An imprint of Elsevier Ltd., Spain, 698-709.
  • Sigstedt SC, Hooten CJ, Callewaert MC, Jenkins AR, Romero AE, Pullin MJ, Kornienko A, Lowrey TK, Slambrouck SV, Steelant WF, 2008: Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells international journal of oncology. International Journal of Oncology, 32, 1085-1090.
  • Stahr HM, 1977: Analytical Toxycology Methods Manual. Ames-Iowa, USA, Iowa State Univ. Press. Sumanth M, Rana AC, 2006: İn vivo antioxidant activity of hydroalcoholic extract of Taraxacum officinale roots in rats. Indian Journal of Pharmacology, 38, 54.
  • Sun Y, Oberley LW, Li Y, 1988: A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34, 497-500.
  • Sundaria K, Karthik D, Ilavenil S, Kaleeswaran B, Srigopalramd S, Ravikumar S, 2013: Hepatoprotective and proteomic mechanism of Sphaeranthus indicus in paracetamol induced hepatotoxicity in wistar rats. Food Bioscience, 57-65. Sümbüloğlu V, Sümbüloğlu K, 1998: Sağlık Bilimlerinde Araştırma Yöntemleri. 2. Baskı. Ankara, Hatiboğlu Yayınları.
  • Szymonik-Lesiuk S, Czechowska G, Stryjecka-Zimmer M, Slomka M, Maldro A, Celinski K, Wielosz M, 2003: Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat tissues after carbon tetrachloride intoxication. Journal of Hepato-Biliary-Pancreatic Surgery, 309-315.
  • Tietze F, 1969: Enzymic method for quantitavite determination of nanogram amounts of total and oxidized glutathione. Analytical Biochemistry, 27, 502-522.
  • Vermeulen NPE, Bessems JGM, Vandestreat R, 1992: Molecular aspects of paracetamolinduced hepatotoxicity and it mechanism based prevention. Drug Metabolism Reviews, 24, 367-407.
  • Yoshioka T, Kawada K, Shimada T, 1979: Lipid peroxidation in materyal and cord blood and prodective mechanism against activated-oxygen toxicity in the blood. American Journal of Obstetrics and Gynecology, 135, 372-376.
  • You Y, Yoo S, Yoon HG, Park J, Lee YH, Kim S, Oh KT, Lee J, Cho HY, Jun W, 2010: In vitro and in vivo hepatoprotective effects of the aqueous extract from Taraxacum officinale (dandelion) root against alcohol induced oxidative stress. Food and Chemical Toxicology, 48, 1632-1637.
  • Zhang L, Maiorino M, Roveri A, Ursini F, 1989: Phospholipid hydroperoxide glutathione peroxidase: specific activity in tissues of rats of different age and comparison with other glutathione peroxidases. Biochim Biophys Acta, 1006, 140-143.

The Effect of Taraxacum officinale Ethanol Extract on Paracetamol Induced Hepatotoxicity in Rats

Yıl 2017, Cilt: 6 Sayı: 1, 11 - 18, 30.06.2017
https://doi.org/10.31196/huvfd.325672

Öz

In
this study was investigated on the effects on the some biochemical and histopathological
parameters in plasma and liver tissue of Taraxacum
officinale
ethanol extract of
paracetamol induced hepatotoxicity in rats.
In this study was utilized 36 Sprague
Dawley male rats, aged 5 months. Rats were divided 6 groups of 6 rats
each, randomly. Animals of paracetamol administered were fasted 24 hour and 2
g/kg peros (p.o) Paracetamol was given after the 1 hour extract was given.
1.Group Control  5% DMSO intraperitoneal (i.p), 2. Group TOE1 200 mg/kg/day/i.p. Taraxacum officinale extract (TOE) was
dissolved in  5% DMSO in distilled water,
i.p., 3. Group TOE2 250 mg/kg/day/i.p. Taraxacum
officinale
extract, 4. Group PARA 2 g/kg/p.o. Paracetamol, 5. Group PTOE1;
Paracetamol 2 g/kg/day/p.o.+ TOE 200 mg/kg/day/i.p, 6. Group PTOE2 Paracetamol
2 g/kg/day/p.o.+ TOE 250 mg/kg/day/i.p were administered for 8 day. At the end
of the study biochemical and histopatological analyses were made from sample of
blood and liver tissue. Plasma
AST, ALT, ALP, MDA, CAT and Nitrite levels; liver tissue Nitrite and Nitrate
levels were increased (P<0.001); plasma GSH (P<0.001), SOD (P<0.001)
and GPx levels (P<0.05); liver tissue GSH, CAT, and SOD levels (P<0.001)
were decreased significantly in paracetamol group
compared with control group.
Paracetamol in rats formed
hepatotoxicity Taraxacum officinale Wig.
aerial part made ​​from ethanol as extract is used TOE 100 mg/kg of the amount
of hepatotoxicity significantly decreased and prevented lipid peroxidation.
Toxic effect was determined in animals administered TOE 250 mg/kg. Even if the obtained
extract had positive effects on hepatotoxicity and biochemical parameters in
liver and plasma, it was concluded that it was needed to for repair the damage
of the liver.

Kaynakça

  • Adam B, 2012: Yiğitoğlu R. Tıbbi Biyokimya, 1. Baskı. İstanbul, Nobel Tıp Kitabevi, 189-249.
  • Aktaş Ö, Eskiocak S, Özgün GS, Yalçın Ö, Süt N, 2013: Asetaminofen ile toksik hepatit oluşturulan ratlarda L-karnitinin etkisi. Turkish Journal of Biochemistry/Türk Biyokimya Dergisi, 38, 475-482.
  • Al-Malki Abdulrahman L, Abo-Golayel MK, 2013: Hepatoprotective Efficacy of Chicory alone or combined with Dandelion leaves against induced liver damage. Life Science Journal, 10(4), 140-157.
  • Albayrak A, 2013: Serotonin-7 reseptörlerinin parasetamol ile indüklenen deneysel karaciğer toksisistesindeki yeri ve önemi. Türk Farmakoloji Derneği Farmakoloji Eğitiminde Kuşaklararası Bilimsel Etkileşme Seminerleri Programı, Bursa.
  • Anonim, 2013a: http://www.naturalfoodbenefits.com /display.asp?CAT=2&I=120. Natural food benefits, Erişim tarihi; 20.09.2013.
  • Anonim,2013b:http://www.naturallifemagazine.com/1204/dandelions.htm. Natural life magazine, Erişim tarihi; 20.09.2013.
  • Ball CR, 1996: Estimation and identification of thiols in rat spleen after cysteine or glutathione treatment pelevance to protection against nitrojen mustards. Biochemical Pharmacology, 15, 809-816.
  • Champe PC, Harvey RA, Ferrier D, 2007: Lippincott’s Illustrated Reviews: Biochemistry. Çeviri: Ulukaya E. Biyokimya, 3. Baskı, İstanbul, Nobel Tıp Kitabevi.
  • Chance B, Greenstein DS, 1992: The mechanism of catalase actions-steady state analysis. Archives of Biochemistry and Biophysics, 37, 301-339.
  • Chattopadhyay RR, 2003: Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: part II. Journal of Ethnopharmacol, 89, 217-219.
  • Cho SY, Oh YJ, Park J, 2003: Effect of dandelion (Taraxacum officinale) leaf extracts on hepatic antioxidative system in rats fed high cholesterol diet. Journal of the Korean Society of Food Science and Nutrition, 2, 458-463.
  • Colle D, Arantes LP, Gubert P, Luz SCA, Athayde ML, Rocha JBT, Soares FAA, 2012: Antioxidant properties of Taraxacum officinale leaf extract are involved in the protective effect against hepatoxicity induced by acetaminophen in mice. Journal of Medicinal Food, 15, 549-556.
  • Davis PH, 1965: Flora of Turkey and The East Aegea Islands, Edinburg, The University Press.
  • Domitrović R, Jakovac H, Romić Ž, Rahelić D, Tadić Ž, 2010: Antifibrotic activity of Taraxacum officinale root in carbon tetrachloride-induced liver damage in mice. Journal of Ethnopharmacology, 130, 569-577.
  • Fernandez V, Videla LA, 1981: Effect of acute and chronic ethanol ingestion on the content of reduced glutathione of varios tissues of the rat. Experientia, 37, 392-394.
  • Fortea MI, Lopez-Miranda S, Serrano-Martinez A, Carreno BJ, Nunez-Delicado E, 2009: Kinetic characterisation and thermal ınactivation study of polyphenol oxidase and peroxidase from table grape (Crimson Seedless). Food Chemistry, 113, 1008-1014.
  • Gallaher RN, Gallaher K, Marshall AJ, 2006: Mineral analysis of ten types of commercially available tea. Journal of Food Composition and Analysis, 19, 53-57.
  • Gonzalez-Castejon M Visioli F, Rodriguez-Casado A, 2012: Diverse biological activities of dandelion. Nutrition Reviews, 70, 534-547.
  • Goth L, 1991: A simple method for determenation of serum catalase activity and revision of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196, 143-152.
  • Hoşbaş S, Hartevioğlu A, Pekcan M, Deliorman Orhan D, 2011: Assessment of hepatoprotective activity of Achillea biebersteinii ethanol extract on carbon tetrachloride-induced liver damage in rats. FABAD Journal of Pharmaceutical Sciences, 36, 33-39.
  • Hu C, Kitts DD, 2004: Luteolin and luteolin-7-O-glucoside from dandelion flower suppress iNOS and COX-2 in RAW264.7 cells. Molecular and Cellular Biochemistry, 265, 107-113. Jaeschke H, Bajt LM, 2006: Intracellular signaling mechanisms of acethominophen-induced liver cell death. Toxicological Sciences, 89, 31-41.
  • Jung K, Henke W, 1996: Developmental changes of antioxidant enzymes activity in kidney and liver from rats. Free Radical Biology and Medicine, 20, 613-617.
  • Kayaalp O, 2005: Analjezikler: Tıbbi Farmakoloji. İçinde: Lippincott: Analgesic Application, 7.Baskı, Ankara, 223-245.
  • Kim MY, Cheong SH, Kim MH, Son C, Yook HS, Sok DE, Cho JHK, ChunH, Kim MR, 2009: Leafy vegetable mix supplementation improves lipid profiles and antioxidant status in C57BL/6J mice fed a high fat and high cholesterol diet. Journal of Medicinal Food, 12, 877-884.
  • Koç H, 2002: Doğrudan, doğadan bitkilerle sağlıklı yaşama. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölüm Yayını, Tokat, Ümit Ofset.
  • Kordali Ş, Çakır A, Akcin TA, Mete E, Akcin A, Aydın T, Kılıç H, 2009: Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Industrial Crops and Products, 29, 562-570.
  • Krishna A, Chaitanya D, Siva RC and Manohar RA, 2012: Hepatoprotective effect of biherbal ethanolic extract against paracetamol-induced hepatic damage in albino rats. Journal of Ayurveda and Integrative Medicine, 198.
  • Kuntz E, Kuntz HD, 2008: Hepatology Textbook and Atlas, 3rd ed. Germany, Springer Press.
  • Matkovics B, Szabo L, Varga IS, 1988: Determination of enzyme activities in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika, 15, 248-249.
  • Nahida T, Qazi MA, Shah A, 2011: Curative activity of ethanol extract of Taraxacum officinale weber. against CCl4 induced hepatocellular damage in albino rats. Journal of Pharmacy Research, 4,3, 687-689.
  • Parmar SR, Dave GS, Patel HV, Kıran K, 2009: Hepato-Protectıve value of some plants extract against carbon tetrachlorıde toxicity in male rats. Journal of Cell and Tissue Research, 9, 1737-1743.
  • Parmar SR, Vashrambhai PH, Kalia K, 2010: Hepatoprotective activity of some plants extract against paracetamol induced hepatotoxicity in rats. Journal of Herbal Medicine and Toxicology, 4, 101-106.
  • Pauli-Magnus C, Stieger B, Meier Y. Kullak-Ublick GA, Meier PJ, 2005: Enterohepatic transport of bile salts and genetics of cholestasis. Journal of Hepatology, 43, 342-357.
  • Placer ZA, Cushman LL, Johnson BC, 1966: Esimaion of product of lipid peroxidaion (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry, 16, 359-364.
  • Plants A and Karaj I. The effects of Taraxacum officinale L. and Berberis vulgaris L. root extracts on carbon tetrachloride induced liver toxicity in rats. Journal of Medicinal Plants, 2010.
  • Raja B, Mol SD, 2010: The protective role of vanillic acid against acetaminophen induced hepatotoxicity in rats. Journal of Pharmacy Research, 1480.
  • Rang HP, Dale MM, Ritter JM, Flower RJ, Henderson G, 2011: Rang&Dale’s Pharmacology. 7th ed. Elsevier Churchill Livingston, An imprint of Elsevier Ltd., Spain, 698-709.
  • Sigstedt SC, Hooten CJ, Callewaert MC, Jenkins AR, Romero AE, Pullin MJ, Kornienko A, Lowrey TK, Slambrouck SV, Steelant WF, 2008: Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells international journal of oncology. International Journal of Oncology, 32, 1085-1090.
  • Stahr HM, 1977: Analytical Toxycology Methods Manual. Ames-Iowa, USA, Iowa State Univ. Press. Sumanth M, Rana AC, 2006: İn vivo antioxidant activity of hydroalcoholic extract of Taraxacum officinale roots in rats. Indian Journal of Pharmacology, 38, 54.
  • Sun Y, Oberley LW, Li Y, 1988: A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34, 497-500.
  • Sundaria K, Karthik D, Ilavenil S, Kaleeswaran B, Srigopalramd S, Ravikumar S, 2013: Hepatoprotective and proteomic mechanism of Sphaeranthus indicus in paracetamol induced hepatotoxicity in wistar rats. Food Bioscience, 57-65. Sümbüloğlu V, Sümbüloğlu K, 1998: Sağlık Bilimlerinde Araştırma Yöntemleri. 2. Baskı. Ankara, Hatiboğlu Yayınları.
  • Szymonik-Lesiuk S, Czechowska G, Stryjecka-Zimmer M, Slomka M, Maldro A, Celinski K, Wielosz M, 2003: Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat tissues after carbon tetrachloride intoxication. Journal of Hepato-Biliary-Pancreatic Surgery, 309-315.
  • Tietze F, 1969: Enzymic method for quantitavite determination of nanogram amounts of total and oxidized glutathione. Analytical Biochemistry, 27, 502-522.
  • Vermeulen NPE, Bessems JGM, Vandestreat R, 1992: Molecular aspects of paracetamolinduced hepatotoxicity and it mechanism based prevention. Drug Metabolism Reviews, 24, 367-407.
  • Yoshioka T, Kawada K, Shimada T, 1979: Lipid peroxidation in materyal and cord blood and prodective mechanism against activated-oxygen toxicity in the blood. American Journal of Obstetrics and Gynecology, 135, 372-376.
  • You Y, Yoo S, Yoon HG, Park J, Lee YH, Kim S, Oh KT, Lee J, Cho HY, Jun W, 2010: In vitro and in vivo hepatoprotective effects of the aqueous extract from Taraxacum officinale (dandelion) root against alcohol induced oxidative stress. Food and Chemical Toxicology, 48, 1632-1637.
  • Zhang L, Maiorino M, Roveri A, Ursini F, 1989: Phospholipid hydroperoxide glutathione peroxidase: specific activity in tissues of rats of different age and comparison with other glutathione peroxidases. Biochim Biophys Acta, 1006, 140-143.
Toplam 47 adet kaynakça vardır.

Ayrıntılar

Bölüm Araştıma
Yazarlar

Esra Aktaş Şenocak Bu kişi benim

Betül Apaydın Yıldırım

Yayımlanma Tarihi 30 Haziran 2017
Gönderilme Tarihi 3 Temmuz 2017
Kabul Tarihi 26 Aralık 2016
Yayımlandığı Sayı Yıl 2017 Cilt: 6 Sayı: 1

Kaynak Göster

APA Aktaş Şenocak, E., & Apaydın Yıldırım, B. (2017). Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi. Harran Üniversitesi Veteriner Fakültesi Dergisi, 6(1), 11-18. https://doi.org/10.31196/huvfd.325672
AMA Aktaş Şenocak E, Apaydın Yıldırım B. Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi. Harran Univ Vet Fak Derg. Haziran 2017;6(1):11-18. doi:10.31196/huvfd.325672
Chicago Aktaş Şenocak, Esra, ve Betül Apaydın Yıldırım. “Ratlarda Parasetamol Ile Oluşturulan Hepatotoksisite Üzerine Taraxacum Officinale Etanol Ekstraktının Etkisi”. Harran Üniversitesi Veteriner Fakültesi Dergisi 6, sy. 1 (Haziran 2017): 11-18. https://doi.org/10.31196/huvfd.325672.
EndNote Aktaş Şenocak E, Apaydın Yıldırım B (01 Haziran 2017) Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi. Harran Üniversitesi Veteriner Fakültesi Dergisi 6 1 11–18.
IEEE E. Aktaş Şenocak ve B. Apaydın Yıldırım, “Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi”, Harran Univ Vet Fak Derg, c. 6, sy. 1, ss. 11–18, 2017, doi: 10.31196/huvfd.325672.
ISNAD Aktaş Şenocak, Esra - Apaydın Yıldırım, Betül. “Ratlarda Parasetamol Ile Oluşturulan Hepatotoksisite Üzerine Taraxacum Officinale Etanol Ekstraktının Etkisi”. Harran Üniversitesi Veteriner Fakültesi Dergisi 6/1 (Haziran 2017), 11-18. https://doi.org/10.31196/huvfd.325672.
JAMA Aktaş Şenocak E, Apaydın Yıldırım B. Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi. Harran Univ Vet Fak Derg. 2017;6:11–18.
MLA Aktaş Şenocak, Esra ve Betül Apaydın Yıldırım. “Ratlarda Parasetamol Ile Oluşturulan Hepatotoksisite Üzerine Taraxacum Officinale Etanol Ekstraktının Etkisi”. Harran Üniversitesi Veteriner Fakültesi Dergisi, c. 6, sy. 1, 2017, ss. 11-18, doi:10.31196/huvfd.325672.
Vancouver Aktaş Şenocak E, Apaydın Yıldırım B. Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi. Harran Univ Vet Fak Derg. 2017;6(1):11-8.